ABSTRACT
A prospective study was carried out in order to assess nosocomial bacteremia in the Intensive Care Unit of the Centrohabana Teaching Pediatric Hospital, from January to May 1988. 66.7% of the bacteremia episodes diagnosed were of a nosocomial origin, mostly secondary. Nosocomial bacteremia rate was 15.5 per 100 admissions, with predominance in the age group under 1 year of age. Risk factors for acquiring nosocomial bacteremia were hospital stay longer than 72 hours, age under 1 year, tracheal intubation, deep venous catheterization and urinary catheterization. The most frequently associated microorganisms were Staphylococcus epidermis, Escherichia coli and Pseudomonas aeruginosa.
Subject(s)
Bacteremia/epidemiology , Cross Infection/epidemiology , Hospitals, Pediatric/statistics & numerical data , Hospitals, Urban/statistics & numerical data , Intensive Care Units, Pediatric/statistics & numerical data , Population Surveillance , Bacteremia/microbiology , Bacteria/isolation & purification , Child , Child, Preschool , Cross Infection/microbiology , Cuba/epidemiology , Humans , Infant , Prospective Studies , Risk FactorsABSTRACT
The microbiologic aspects of the bacteriemia were prospectively studied in patients hospitalized in a pediatric Intensive Care Unit. Thirty six episodes of bacteriemia were detected in 29 patients. Secondary bacteriemia prevailed on the primary ones. The most frequent infectious foci associated with bacteriemia were the infections of respiratory tract, followed by intravascular catheterism. The microorganisms more frequently associated with bacteriemia were: Haemophilus influenzae, Staphylococcus epidermidis, Eacherichia coli and Pseudomonas seruginosa.
Subject(s)
Intensive Care Units, Pediatric , Sepsis/microbiology , Child, Preschool , Hospitalization , Humans , Prospective StudiesABSTRACT
A study is made of non-fermenting gram-negative bacterial strains isolated at the Microbiology Lab, "Centro Habana" Pediatric Teaching Hospital. The strains studied come from various types of samples with prevalence of exudates and smears. A definite biotyping of 100% of strains is made. 90.0% are classified within the genus Pseudomonas. In addition, organisms of the genera Acinetobacter, Alcaligenes, Achromobacter, and Moraxella are identified. The most frequent species turned out to be Pseudomonas aeruginosa, which accounted for 68.4% of the total.
Subject(s)
Gram-Negative Aerobic Bacteria/classification , Bacterial Typing Techniques , Cuba , Fermentation , Gram-Negative Aerobic Bacteria/metabolism , Hospitals, Pediatric , HumansABSTRACT
The use of King A and King B media for the faster diagnosis of Pseudomonas aeruginosa is introduced in the Microbiology Laboratory of Centro Habana Pediatric Teaching Hospital. The appropriate use of these media allows to classify as Pseudomonas aeruginosa 77.2% of the Pseudomonas sp. strains studied, which represent 95.2% of the total of strains identified as Pseudomonas aeruginosa, according to Hugh and Gilardi conventional technique. There are no significant differences in the implementation of the two procedures (X2: 0.5; p less than 0.05). The demonstrations of pyocines with King A medium is significantly superior to the method for extraction and solubility of the pigment in chloroform (X2: 15.05; p less than 0.01).
Subject(s)
Culture Media , Oligopeptides , Pseudomonas Infections/diagnosis , Pseudomonas aeruginosa/isolation & purification , Bacterial Typing Techniques/methods , Cuba , Hospitals, Pediatric , Hospitals, Teaching , Humans , Pigments, Biological/analysis , Pigments, Biological/biosynthesis , Pseudomonas/classification , Pseudomonas aeruginosa/metabolism , Pyocyanine/analysis , Pyocyanine/biosynthesisABSTRACT
The authors worked with 2,273 strains of Staphylococcus aureus isolated from clinical samples processed at the Microbiology laboratory of Centro Habana Pediatric Teaching Hospital in the period ranging from June 1985 to December 1986. All strains underwent the bacterial sensitivity test against antibiotics by Bauer-Kirby technique. Fourty-eight strains were sensitive to penicillin - which accounts for 2.11% - by the beta-lactamase detection test by the starch paper method. In all cases, negative results were obtained, which is in agreement with the results of the sensitivity tests.
Subject(s)
Penicillin Resistance , Penicillinase/isolation & purification , Staphylococcus aureus/enzymology , Microbial Sensitivity Tests , Staphylococcus aureus/drug effects , StarchABSTRACT
Se realizo la busqueda de anticuerpos aglutinantes antipertussis en 480 sueros sanguineos, procedentes de ninos de 0 a 14 anos, inmunizados con la vacuna triple (DPT) en la Ciudad de La Habana.Se utilizo la tecnica de aglutinacion en tubo, con diluciones de suero al duplo, utilizando 10 tubos, se comenzo con la dilucion de 1:20. Se empleo el antigeno para la reaccion de aglutinacion de Bortedella pertussis preparado en el INHEM. De los 480 sueros estudiados, en el 71% se observo aglutinacion y en 174 (36,2%) con titulos de 1:320 o mas. Se relacionan los resultados del estudio serico con los grupos de edades
