[Effect of millimeter waves on the early development of the mouse and sea urchin embryo]. / Deistvie millimetrovykh voln na rannee razvitie zarodyshei myshei i morskikh ezhei.

The action of nonthermal electromagnetic radiation (EMR) of the millimeter range on the early development of murine and sea urchin embryos was investigated. An MRTA-01E-03 generator with a frequency of 54-78 GHz and radiation intensity of 0.06 mWt/cm2 was used. The embryos were irradiated during 30 min at the stage of two blastomeres. The number of murine embryos that reached the blastocyst stage increased (up to 97.3% in comparison with 87.5% in control). The total time of cultivation up to the blastocyst stage was also shorter (72 h) than in control (96 h). The irradiation had effect on the development of sea urchin embryos only if embryos with a weakened viability were tested. The results indicate that millimeter electromagnetic radiation has a stimulating effect on the early development of embryos, increasing the resistance of embryos to unfavorable environmental conditions.

[Nonsurgical embryo transplantation in mice]. / Nekhirurgicheskaia transplantatsiia zarodyshei u myshei.

Nonsurgical transplantation of the mouse embryos at the blastocyst stage into the uterus was performed using a device of original construction. The use of this device made it possible to exclude the loss of embryos, to reduce the amount of culture medium introduced together with the embryos, to lessen the damage of genital tract and ensured guaranteed "ejection" of embryos into the uterus. The females of the third day of pregnancy were used of recipients. Transplantation was performed between the mice of strains CBA/Lac, C57BL/6 and tetrahybrid CBWA. The level of newborn attained 65% of the number of transplanted blastocysts.