ABSTRACT
AIM: Evaluate resistance to working solutions of disinfectants by Staphylococcus haemolyticus and Klebsiella pneumoniae isolated from newborns and hospital environment objects of obstetric hospital during registration of group purulent-septic infections (PSI). MATERIALS AND METHODS: Analysis of 2 epidemic situations on PSI morbidity of newborns caused by S. haemolyticus and K. pneumoniae was carried out. Sensitivity to antibiotics of S. haemolyticus and K. pneumoniae strains isolated from newborns and hospital environment was studied by disc-diffusion method and genotyping of K. pneumoniae--by using polymerase chain reaction with universal primer M 13 (RAPD-PCR). Sensitivity of S. haemolyticus and K. pneumoniae to working solutions of disinfectants was determined on test-surfaces (glass, metal, plastic, wood, oilcloth). RESULTS: The detected identity of antibiotic phenotype of S. haemolyticus and K. pneumoniae strains as well as genotype of K. pneumoniae strains combined with registration of group PSI morbidity among newborns confirms that the circulating strains (clones) of the causative agents were hospital. S. haemolyticus and K. pneumoniae strains in most cases were sensitive to working solutions of disinfectants. CONCLUSION: Resistance of causative agents of nosocomial PSI to disinfectants is not an unconditional feature of a hospital strain, and concurrence of resistance profile of microorganisms to disinfectant preparations--a mandatory feature of the presence of epidemiologic connection between the diseased.
Subject(s)
Cross Infection/prevention & control , Disinfectants/pharmacology , Klebsiella Infections/prevention & control , Microbial Sensitivity Tests , Cross Infection/microbiology , Cross Infection/pathology , Drug Resistance, Bacterial/genetics , Humans , Infant, Newborn , Klebsiella Infections/microbiology , Klebsiella pneumoniae/drug effects , Klebsiella pneumoniae/genetics , Klebsiella pneumoniae/pathogenicity , Staphylococcus haemolyticus/drug effects , Staphylococcus haemolyticus/genetics , Staphylococcus haemolyticus/pathogenicitySubject(s)
Electroencephalography , Individuality , Adaptation, Physiological , Dominance, Cerebral/physiology , Electroencephalography/methods , Electroencephalography/statistics & numerical data , Humans , Occupational Exposure/adverse effects , Occupational Exposure/statistics & numerical data , Petroleum , Time FactorsABSTRACT
The EEG, quantity of cation proteins in neutrophils, the opioid peptides concentration in the CSF, were analysed in military personnel after a prolonged climatic and specific stress complicated by traumas, at the Kabul military hospital in Afghanistan. The brain functional reserve was significantly lower in the control subjects. A positive correlation between the brain functional reserve and the average cytochemical coefficient, was found in certain phases of traumatic disease alone. Accumulation of opioid peptides occurred under the adaptation to stress, being more evident in wounded soldiers.
