1.
Nucleosides Nucleotides Nucleic Acids
; 20(4-7): 977-9, 2001.
Article
in English
| MEDLINE
| ID: mdl-11563158
ABSTRACT
The preparation of nucleosides as well as their base-modified analogues using purified nucleoside phosphorylase enzymes or, more conveniently, using whole bacterial cells is described. The development of genetically modified strains of Escherichia coli, able to over-produce Uridine-phosphorylase and Purine-nucleoside-phosphorylase in the same cells, improves the specific biocatalytic activity and the consequent industrial scale approach.