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1.
ACS Appl Mater Interfaces ; 15(47): 55082-55094, 2023 Nov 29.
Article in English | MEDLINE | ID: mdl-37936415

ABSTRACT

Strong electromagnetic and heat flux stresses can induce severe damage to solid insulation materials, leading to faults in power equipment and power electronics devices. However, in the absence of suitable in situ imaging methods for observing the development and morphology of electrical damage within insulation materials, the mechanism of insulation failure under high-frequency electric fields has remained elusive. In this work, a recently discovered fluorescence self-excitation phenomenon in electrical damage channels of polymers is used as the basis for a laser confocal imaging method that is able to realize three-dimensional (3D) in situ imaging of electrical tree channels in silicone gel through nondestructive means. Based on the reconstructed morphology of the damaged area, a spatial equivalent calculation model is proposed for analysis of the 3D geometric features of electrical trees. The insulation failure mechanism of silicone gel under electric fields of different frequencies is analyzed through ReaxFF molecular dynamics simulations of the thermal cracking process. This work provides a new method for in situ nondestructive 3D imaging of micro/nanoscale damage structures within polymers with potential applications to material analysis and defect diagnosis.

2.
Ying Yong Sheng Tai Xue Bao ; 32(6): 2028-2034, 2021 Jun.
Article in Chinese | MEDLINE | ID: mdl-34212608

ABSTRACT

To analyze the feeding habits and trophic level of jellyfish (Rhopilema esculentum), Chinese shrimp (Fenneropenaeus chinesis), grass shrimp (Penaeus monodon), and clam (Ruditapes philippinarum), and the food web structure in marine aquaculture pond, we measured the δ13C and δ15N values of the four species and different feed from May to September in 2017. The average proportional contribution of different feed to the four species were analyzed using the IsoSource linear mixture model. The results showed that zooplankton was the main food source to jellyfish, Engraulis japonicus was the main food source to the two shrimp species, and the phytoplankton, benthic diatoms and dejecta of the two shrimp species were the main food source to the clam. The trophic level of the clam ranged from 2.64 to 2.95, with a mean value of 2.84. The trophic level of jellyfish ranged from 2.78 to 3.27, with a mean value of 3.06. the grass shrimp ranged from 3.03 to 3.54, with a mean value of 3.25. The trophic level of Chinese shrimp ranged from 3.76 to 4.40, with a mean value of 3.95. Results of comprehensive analysis showed that the clam was the primary consumer, jellyfish was the secondary consumer, and shrimps were the predators. Jellyfish filtered the dejecta of the two kinds of prawns and improved water quality of the polyculture pond to a certain extent.


Subject(s)
Food Chain , Ponds , Animals , Nitrogen Isotopes/analysis , Shellfish , Zooplankton
3.
Neurosci Lett ; 633: 279-289, 2016 10 28.
Article in English | MEDLINE | ID: mdl-27721206

ABSTRACT

The cortical thickness has gained an extensive attention as a pathological alteration of sporadic Parkinson's disease (sPD), the alteration of pathological cortical thickness may distinctly contribute to the consistent clinical manifestations. Therefore, we investigated the cortical thickness correlates of clinical manifestations in the mid-stage sPD from the Han population of Chinese mainland (HPCM). A sample of 67 mid-stage sPD patients and 35 matched controls from HPCM were performed a corticometry of magnetic resonance imaging (MRI) and the assessment of clinical manifestations including the demographic and disease-related characteristics, and underwent the final analysis of the cortical thickness correlates with the clinical manifestations. In our result, we demonstrated that no significant differences in the demographic characteristics were found among the two groups. The tests of clinical disease-related characteristics demonstrated that the significant differences in the Hoehn and Yahr scale, the UPDRS Part I-IV, the symptom-dominant side (right/left/double), the tremor subscoree off (e), the tremor subscoref on (f), Webster, MMSE, HDS-R, DF, DB, SVFT, SDS, HAMD17, HAMD 24, CDT, CDR, LEDD and PDSI were observed between the mid-stage sPD patients and the controls. The analysis about the cortical thickness correlates with the clinical manifestations revealed that a significant correlation between UPDRS-I and Frontal-Sup-Orb-R and Rectus-R; DB and Frontal-Sup-Orb-R and Frontal-Inf-Orb-R; SDS and Frontal-Sup-Orb-R, Frontal-Mid-Orb-R, Rectus-R and Cingulum-Ant-R respectively in the mid-stage sPD patients from HPCM. Our data showed that the cortical thinning in the right frontal Orb, rectus and cingulum were the pathological base of some clinical manifestations including the cognitive impairment, hallucinations, psychosis, the depressed mood, the anxious mood, apathy, the sleep problems, the nighttime or/and daytime sleepiness, the short term memory stores and the central execution, as well as the sexual desire disorder in the mid-stage sPD patients, suggesting that the dysfunctions of brain regions of some cortical thinning are closely correlated with some clinical manifestations of the mid-stage sPD.


Subject(s)
Cerebral Cortex/pathology , Parkinson Disease/pathology , Parkinson Disease/physiopathology , Aged , Case-Control Studies , Female , Humans , Magnetic Resonance Imaging , Male , Parkinson Disease/diagnosis
4.
Yi Chuan ; 31(6): 629-37, 2009 Jun.
Article in Chinese | MEDLINE | ID: mdl-19586864

ABSTRACT

AFLP markers were used to construct the primary linkage map in a family of Mizuhopecten yessoensis. A total of 1 855 markers were generated in two parents and 52 progenies of the mapping family by using 56 AFLP primer combinations. Among the 1 855 markers, 598 were polymorphic and 354 were in agreement with the Mendelian segregating ratio of 1:1. Markers segregated according to Mendelian 1:1 ratio (P>0.05) and 23 distorted markers (0.01

Subject(s)
Amplified Fragment Length Polymorphism Analysis , Crosses, Genetic , Genetic Linkage , Pectinidae/genetics , Animals , DNA/analysis , Female , Genetics, Population , Japan , Male , Polymorphism, Genetic
5.
Yi Chuan ; 30(11): 1453-8, 2008 Nov.
Article in Chinese | MEDLINE | ID: mdl-19073555

ABSTRACT

MYP (Major yolk protein) gene expression at transcription level in different stages of gonad of sea urchin Strongylocentrotus intermedius and hybrids (S. intermediusfemale symbolxS. nudusmale symbol) was analyzed by real-time RT-PCR. Based on normalization with 18S rRNA levels, the comparative quantities of MYP expression were determined. The expression of MYP gene in gonad showed little difference between female and male. MYP gene expression was decreased rapidly in the gonad of S. intermedius at different stages, and slowly in hybrids. The comparative quantities of MYP expression in the gonads of S. intermedius were decreased from 44.55% to 9.59% in female and from 41.17% to 1.83% in male at different stages. The comparative quantities of MYP expression in the gonads of the hybrids were decreased from 37.66% to 19.22% in female and from 36.66% to 12.55% in male at different stages. The results indicated that the difference of MYP expression was correlated with the variation caused by hybridization.


Subject(s)
Egg Proteins/genetics , Gene Expression Regulation, Developmental , Gonads/growth & development , Gonads/metabolism , Strongylocentrotus/growth & development , Strongylocentrotus/genetics , Transcription, Genetic , Animals , Female , Gonads/cytology , Hybridization, Genetic , Male , Polymerase Chain Reaction , RNA, Ribosomal, 18S/biosynthesis , Reference Standards
6.
Yi Chuan ; 29(7): 829-36, 2007 Jul.
Article in Chinese | MEDLINE | ID: mdl-17646149

ABSTRACT

Spotted halibut (Verasper variegatus) is the only species of Genus Verasper in China. The fish was naturally distributed in Yellow Sea and Bohai Sea in northern China and Kyushu in Japan and in Korean sea area. Using PCR product direct sequencing, mitochondrial control region sequences of 24 individuals of spotted halibut was confirmed and analyzed. 4 control region haplotypes, resulting from length heteroplamy of the tandem repeat region, was obtained from these 24 fish. Sequence analysis demonstrated that there were four similar structures in the control region, i.e., extended terminal associated sequences (ETAS), central conserved sequence block (CSB), conserved sequence block (CSB), and repeat region, in V. moseri, Limanda ferruginea, Reinhardtius hippoglossoides, Heppoglossoides platessoides, Paralichthys olivaceous, Solea solea, S. senegalensis, and S. lascari. By comparing with other vertebrates, we found that there were similar repeated sequences immediately after the CSB-3 in all of the anuran species.


Subject(s)
DNA, Mitochondrial/genetics , Flounder/genetics , Animals , Base Sequence , DNA, Mitochondrial/chemistry , Flounder/classification , Molecular Sequence Data , Phylogeny , Polymerase Chain Reaction , Sequence Analysis, DNA , Sequence Homology, Nucleic Acid , Tandem Repeat Sequences/genetics
7.
Yi Chuan ; 29(4): 443-8, 2007 Apr.
Article in Chinese | MEDLINE | ID: mdl-17548307

ABSTRACT

AFLP analysis of genetic diversity in the three populations of sea urchin Strongylocentrotus intermedius (IN), S. nudus (NU) and F1 progeny (INxNU) was carried out in this paper. In total, 272 loci were amplified with 4 primer pairs, of which 269 were polymorphic and the percentage of polymorphic loci was 98.89%. The Shannon diversity index for S. intermedius, S. nudus, and their hybrid populations was 0.2331+/-0.1273, 0.2005+/-0.1385, and 0.2625+/-0.1067, respectively. The genetic similarity within populations was 0.6876+/-0.0523, 0.6501+/-0.0548, and 0.6552+/-0.0553, respectively. AMOVA analysis indicated that 25.39% of variance was among populations and 74.61% of variance was within populations. This suggested the rich genetic diversity level within populations. Although the hybrids can be classified into two types by apparent characters, they were clustered each other by UPGMA method according to their genetic distances.


Subject(s)
Amplified Fragment Length Polymorphism Analysis/methods , Chimera , Strongylocentrotus/genetics , Animals , Chimera/metabolism , Phylogeny , Polymorphism, Restriction Fragment Length , Random Amplified Polymorphic DNA Technique , Sea Urchins/genetics
8.
Guang Pu Xue Yu Guang Pu Fen Xi ; 27(3): 595-7, 2007 Mar.
Article in Chinese | MEDLINE | ID: mdl-17554931

ABSTRACT

A study was carried out on the contents of nutritional elements such as K, Fe, Zn, Ca, Cu, Mg and Mn in three different red pear varieties, namely Meirensu, Hongsucui and Mantianhongs, by flame atomic absorption. The results indicated that the linear relationships for different elements within the limits of working curves are good, and the range of recovery is 98%-104%, hence showing that the results asre satisfactory. There are abundant nutritional elements in fruits of the three different red pear varieties, meaning that they all have a relatively high nutritive value. In fruits of the three red pear varieties, the content sequence of different nutritional elements was found to be K>Fe>Zn>Ca>Cu>Mg>Mn. In fruits of the three different red pear varieties, the content sequence of K, Zn, Mg and Mn was Meirensu> Hongsucui>Mantianhong; the content sequence of Fe and Cu was Hongsucui>Mantianhong>Meirensu; and the content sequence of Ca was Mantianhong>Hongsucui> Meirensu; and the total content sequence of seven nutritional elements was Hongsucui>Meirensu>Mantianhong.


Subject(s)
Calcium/analysis , Fruit/chemistry , Magnesium/analysis , Metals, Heavy/analysis , Potassium/analysis , Pyrus/chemistry , Spectrophotometry, Atomic/methods , Color
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