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1.
Neurology ; 71(21): 1709-12, 2008 Nov 18.
Article in English | MEDLINE | ID: mdl-19015486

ABSTRACT

OBJECTIVE: Kufs disease is the adult-onset form of neuronal ceroid lipofuscinosis (NCL). Its two clinical phenotypes are type A (progressive myoclonus epilepsy with dementia) and type B (behavioral abnormalities and dementia, associated with pyramidal and extrapyramidal signs). METHODS: We describe the clinical evolution of an atypical case characterized by progressive dementia and focal occipital seizures. RESULTS: A healthy 37-year-old woman began showing memory deficits and behavioral disturbances (apathy, lack of inhibitions, untidiness). After 4 years, she developed rare clusters of tonic-clonic seizures, as well as focal seizures originating from the temporo-occipital regions, clinically associated with visual hallucinations, wandering, and agitation. When she was 44 years old, neuropsychological assessment revealed severe frontotemporal dementia. MRI showed cortical atrophy and, on T2-weighted images, hypointensity of the basal ganglia, and hyperintensity and reduction of the deep white matter. On the basis of these findings, a diagnosis of Kufs disease was hypothesized. A skin biopsy was negative, but electron microscopy examination of a right frontal lobe brain biopsy revealed the presence of typical storage material (fingerprint inclusions). The patient never developed myoclonus or extrapyramidal signs. DISCUSSION: Kufs disease is difficult to diagnose on account of its heterogeneous clinical pattern and pathologic features, and the lack of a specific genetic locus alteration. The neuropsychological pattern and MRI findings observed in patients with early-onset frontotemporal dementia and seizure disorder suggest that Kufs disease should be considered in their differential diagnosis. Extracerebral biopsy can be nondiagnostic, and when alternative diagnoses have been ruled out, cerebral biopsy should be considered.


Subject(s)
Dementia/complications , Epilepsies, Partial/complications , Neuronal Ceroid-Lipofuscinoses/diagnosis , Adult , Disease Progression , Electroencephalography/methods , Female , Humans , Magnetic Resonance Imaging/methods , Neuronal Ceroid-Lipofuscinoses/etiology
2.
Neurobiol Aging ; 29(6): 926-36, 2008 Jun.
Article in English | MEDLINE | ID: mdl-17258840

ABSTRACT

In an autopsy series of 19 individuals, age-ranged 24-94, a relatively age-spared region, the anterior-ventral thalamus, was analyzed by immunohistochemical techniques to visualize neurons (neurofilament protein), astrocytes (glial fibrillary acidic protein), microglial cells (CD68) and amyloid precursor protein. The pattern of immunoreactivity was determined by surface fractal dimension and lacunarity, the size by the field area (FA) and the spatial uniformity by the uniformity index. From the normalized FA values of immunoreactivity for the four markers studied, a global parameter was defined to give an overall characterization of the age-dependent changes in the glio-neuronal networks. A significant exponential decline of the GP was observed with increasing age. This finding suggests that early in life (age<50 years) an adaptive response might be triggered, involving the glio-neuronal networks in plastic adaptive adjustments to cope with the environmental challenges and the continuous wearing off of the neuronal structures. The slow decay of the GP observed in a later phase (age>70 years) could be due to the non-trophic reserve still available.


Subject(s)
Aging/metabolism , Aging/pathology , Neuroglia/cytology , Neuroglia/metabolism , Neurons/cytology , Neurons/metabolism , Thalamus/cytology , Thalamus/metabolism , Adult , Aged , Aged, 80 and over , Humans , Male , Middle Aged
3.
Curr Protein Pept Sci ; 7(1): 3-15, 2006 Feb.
Article in English | MEDLINE | ID: mdl-16472166

ABSTRACT

Proteins are endowed with the "Lego property", i.e., the capability of steric fitting with other proteins to form high molecular weight complexes with emergent functions. These interactions may occur both as horizontal molecular networks at the plasma membrane level and as vertical molecular networks, i.e., towards the extra- and/or intracellular side of the cell. The present paper broadens this view by proposing the existence of three dimensional molecular networks, mainly made by proteins and carbohydrates, which might interact with each other at boundaries of compartments such as plasma membranes to form a "global molecular network" (GMN) that pervades the intra- as well as the extra-cellular environment of the entire central nervous system. The GMN is a potentially plastic structure regulated through several means. For example, its extra-cellular part is under the remodeling action of the matrix metalloproteinases. The proposal of a GMN has physiological and pathological implications. In primis, classical synaptic transmission, gap junctions and volume transmission signals by modulating GMN could importantly contribute to the "binding phenomenon", i.e. the phase synchronization of firing rates in far-located neuronal cortical groups. Secondly, alterations in protein conformation could alter the GMN organization and hence the neuronal network morphology and function. This could lead to the formation of abnormal protein aggregates such as amyloid plaques and neurofibrillary tangles, which, in turn, might affect the GMN function and/or the reciprocal interactions between its parts especially at the boundaries between compartments.


Subject(s)
Central Nervous System/physiology , Central Nervous System/physiopathology , Animals , Carbohydrates/physiology , Central Nervous System/cytology , Gap Junctions/physiology , Humans , Models, Neurological , Neurons/cytology , Neurons/physiology , Protein Conformation , Proteins/chemistry , Proteins/metabolism , Signal Transduction , Synapses/physiology
4.
Int J Mol Med ; 16(2): 301-7, 2005 Aug.
Article in English | MEDLINE | ID: mdl-16012766

ABSTRACT

The present case report was aimed at identifying the molecular profile characteristic of a primitive neuro-ectodermal tumor (PNET) in a 3-year-old child affected by a lesion localized in the cerebellar region. The histological diagnosis was medulloblastoma. In vivo single voxel 1H magnetic resonance spectroscopy (MRS) shows high specificity in detecting the main metabolic alterations in the primitive cerebellar lesion; a very high amount of the choline-containing compounds and very low level of creatine derivatives and N-acetylaspartate. Ex vivo high resolution magic angle spinning (HR-MAS) 1H magnetic resonance spectroscopy, performed at 9.4 Tesla on the neoplastic specimen collected during surgery, allows for the unambiguous identification of several metabolites giving a more in-depth evaluation of the metabolic pattern of the lesion. The ex vivo HR-MAS MR spectra show that the spectral detail is much higher than that obtained in vivo and that, for example, myo-inositol, taurine and phosphorylethanolamine contribute to the in vivo signal at 3.2 ppm, usually attributed to choline-containing compounds. In addition, the spectroscopic data appear to correlate with some morphological features of the medulloblastoma. Consequently, the present study shows that ex vivo HR-MAS 1H MRS is able to strongly improve the clinical possibility of in vivo MRS and can be used in conjunction with in vivo spectroscopy for clinical purposes.


Subject(s)
Cerebellar Neoplasms/diagnosis , Magnetic Resonance Spectroscopy/methods , Medulloblastoma/diagnosis , Neuroectodermal Tumors, Primitive/diagnosis , CD56 Antigen/analysis , Cerebellar Neoplasms/metabolism , Child, Preschool , Diagnosis, Differential , Humans , Immunohistochemistry , Male , Medulloblastoma/metabolism , Neuroectodermal Tumors, Primitive/metabolism
5.
Pathol Res Pract ; 197(4): 231-6, 2001.
Article in English | MEDLINE | ID: mdl-11358008

ABSTRACT

p27Kip1, a cyclin-dependent kinase inhibitor, is considered to be a tumor suppressor gene. Absent or reduced expression of the p27Kip1 protein has been reported being a negative prognostic marker in primary lung, breast, colon, bladder, and prostate carcinomas. p27Kip1 protein expression was evaluated in a series of 96 gastric carcinomas with no lymph node involvement (NO) to verify any impact on the clinical outcome. The analysis also considered the classic clinico-pathological parameters, such as age, sex, and depth of tumor invasion (pT). The most widely used classification systems for gastric carcinoma were adopted. The expression of p27pKip1 was related neither to the pT category nor to tumor histology. Kaplan-Meier analysis documented a significant impact of an advanced pT category (p < 0.0001) and p27Kip1-reduced expression (p < 0.0002) on survival. Multivariate analysis confirmed that the reduced p27Kip1 protein expression was a strong independent predictor of poor outcome, ranking second to the pT category only (p < 0.006 and p < 0.004 respectively). As reported for other neoplasms, the expression of p27Kip1 appears to be associated with the clinical outcome of gastric carcinoma.


Subject(s)
Adenocarcinoma/metabolism , Microfilament Proteins/metabolism , Muscle Proteins , Stomach Neoplasms/metabolism , Adenocarcinoma/mortality , Adenocarcinoma/pathology , Adenocarcinoma/surgery , Adult , Aged , Aged, 80 and over , Female , Humans , Immunoenzyme Techniques , Lymph Node Excision , Male , Middle Aged , Stomach Neoplasms/mortality , Stomach Neoplasms/pathology , Stomach Neoplasms/surgery , Survival Rate
6.
Breast Cancer Res Treat ; 63(3): 193-8, 2000 Oct.
Article in English | MEDLINE | ID: mdl-11110053

ABSTRACT

There is preliminary evidence that polymorphism of apolipoprotein E (apoE, protein; APOE, gene), one of the key regulatory proteins in cholesterol metabolism, influences the pathobiology of carcinoma of the colon, prostate and breast and also primary tumours of the brain. This study was designed to determine whether APOE polymorphism is related to variation in the rate of tumour cell proliferation and clinical outcome in carcinoma of the breast. One hundred and eleven infiltrating ductal carcinomas, for which follow up data were available, were included in the study. Estrogen and progesterone receptor status (ER, PR) cell proliferation index (MIB- 1) and APOE genotypes were determined from paraffin-embedded tissue by standard methods. Positive correlations were found between grade and tumour size, grade and presence of metastasis, grade and MIB-1 expression, as well as between ER and PR. Survival correlated inversely with tumour size and the presence of positive lymph nodes. Both steroid receptors correlated inversely with MIB- 1 expression. PR positive status also correlated inversely with high histological grade and presence of lymph node metastases. APOE allele frequencies resembled those of the general population. No significant associations were found between possession of either APOE epsilon2 or epsilon4 alleles and the parameters investigated. Although there is evidence to suggest that APOE epsilon4 may predispose to the development of carcinoma of the breast our data do not support the hypothesis that APOE genotype influences the rate of tumour cell proliferation or the clinical course.


Subject(s)
Apolipoproteins E/genetics , Breast Neoplasms/genetics , Polymorphism, Genetic , Adult , Aged , Aged, 80 and over , Alleles , Antigens, Nuclear , Breast Neoplasms/pathology , Cell Division , Female , Humans , Ki-67 Antigen , Middle Aged , Neoplasm Staging , Nuclear Proteins/analysis , Receptors, Estrogen/analysis
7.
Clin Neuropathol ; 19(1): 1-6, 2000.
Article in English | MEDLINE | ID: mdl-10774944

ABSTRACT

AIMS: This study was designed to determine whether the polymorphism of apolipoprotein E (apoE), one of the key regulatory proteins in cholesterol metabolism, is related to varying susceptibility to central nervous system (CNS) neoplasms, and to evaluate any possible interaction between this polymorphism and tumor cell proliferation or clinical outcome. METHODS AND RESULTS: 53 CNS tumors were selected. Follow-up and survival data were available for 36 patients. ApoE genotypes and cell proliferation indices (nucleolar organizer regions, MIB-1, PCNA, p53) were determined from paraffin-embedded tissue by standard methods. Each of the indices of cell proliferation correlated positively with tumor grade and negatively with duration of clinical follow-up and survival. There was a non-significant trend for apoE epsilon2 allele carriers to have high-grade tumors and apoE epsilon4 allele carriers to have low-grade tumors. Possession of apoE epsilon4 was associated with a more advanced age of disease presentation (p < 0.01) and a longer duration of follow-up (p < 0.04). No significant correlations were found between possession of either apoE epsilon2 or apoE epsilon4 alleles and indices of cell proliferation. CONCLUSIONS: These preliminary findings suggest that possession of apoE epsilon4 allele may correspond to a more favorable clinical course in terms of more advanced age of disease presentation, and longer duration of follow-up and survival in patients with CNS neoplasms.


Subject(s)
Apolipoproteins E/genetics , Cell Division/genetics , Central Nervous System Neoplasms/genetics , Polymorphism, Genetic/genetics , Adolescent , Adult , Age Factors , Aged , Brain/pathology , Central Nervous System Neoplasms/pathology , Child , Female , Genotype , Humans , Male , Middle Aged , Spinal Cord/pathology
8.
Panminerva Med ; 41(2): 175-8, 1999 Jun.
Article in English | MEDLINE | ID: mdl-10479920

ABSTRACT

Myelolipomas are very rare benign tumours composed of an admixture of mature adipose tissue and normal haematopoietic cells. Although they are most commonly found in the adrenal glands, extra-adrenal myelolipomas are documented. We described a case of myelolipoma arising in the lung in a 52-year-old man. The lesion was found incidentally in association with a carcinoid. To our knowledge, this is the second instance of this neoplasm presenting as a lung lesion, and the first case associated with bronchial carcinoid. Pathogenesis and aetiology of myelolipomas are referred to in this paper with special regard to the clinical and pathological findings.


Subject(s)
Bronchial Neoplasms/complications , Carcinoid Tumor/complications , Lung Neoplasms/complications , Myelolipoma/complications , Diagnosis, Differential , Hematologic Diseases/diagnosis , Humans , Lung Neoplasms/diagnosis , Lung Neoplasms/pathology , Male , Middle Aged , Myelolipoma/diagnosis , Myelolipoma/pathology
9.
Article in English | MEDLINE | ID: mdl-9503455

ABSTRACT

To find a better method for predicting the biological behavior of certain oral cavity lesions, the expression of nucleolar protein p120 and nucleolar organizer region counts (AgNOR) was compared with that of nuclear proliferation markers MIB-1 and PCNA in 10 cases of keratotic epithelial hyperplasia (KEH), 10 cases of epithelial dysplasia (ED), and 15 cases of squamous cell carcinoma (SCC). Significant differences in p120 and AgNOR mean area values and PCNA labeling index (LI) were recorded between KEH and ED, as well as ED and SCC (Student-Neumann-Keuls test). All markers significantly differed between SCC grades I and III. Significant differences were also noted in AgNOR mean area values between grade I and II SCC and in p120 mean area values. MIB-1 and PCNA LI differed significantly when grade II and III SCC were compared (SNK test). There were significant correlations between p120 and AgNOR (Pearson correlation coefficients) and between both of them and the proliferative indexes. AgNOR correlated with tumor grade, stage, and lymph node status (Spearman correlation coefficients), suggesting a prognostic role for that marker.


Subject(s)
Biomarkers, Tumor/analysis , Mouth Diseases/immunology , Mouth Neoplasms/immunology , Nucleolus Organizer Region/pathology , Analysis of Variance , Antigens, Neoplasm/analysis , Antigens, Nuclear , Humans , Hyperplasia/immunology , Ki-67 Antigen , Nuclear Proteins/analysis , Nucleolus Organizer Region/chemistry , Prognosis , Proliferating Cell Nuclear Antigen/analysis , Retrospective Studies , Silver Staining , Statistics, Nonparametric , tRNA Methyltransferases
10.
Histochem J ; 29(9): 661-8, 1997 Sep.
Article in English | MEDLINE | ID: mdl-9413739

ABSTRACT

The monoclonal antibody FB-2 recognizes the antigen p120-kDa protein (p120), associated with the nucleolar matrix. p120 has originally been reported as expressed and detectable in malignant and non-neoplastic proliferating cells, but not in most normal resting tissues and benign tumours. In the present study, a reliable immunostaining method was used to detect p120 on formalin-fixed, paraffin wax-embedded tissue, testing it on 148 samples from different neoplastic and non-neoplastic tissues from different organs (breast, colon, lung, prostate, bladder, lymph nodes, skin, tongue and liver). The immunostaining was performed after the application of a specific antigen-unmasking protocol based on six consecutive cycles of microwave oven heating. Under these retrieval conditions, p120 antigen was clearly detectable, not only in hyperplastic and malignant cells, but also in stromal and normal non-proliferating cells of all the tissues evaluated. Our results show that the nucleolar protein p120 can be detected by routine immunohistochemistry in formalin-fixed, paraffin-embedded tissue and is expressed in all nucleated cells under any biological condition.


Subject(s)
Antigens, Neoplasm/analysis , Biomarkers, Tumor/analysis , Neoplasms/metabolism , Nuclear Proteins/analysis , Colon/metabolism , Colon/pathology , Humans , Hyperplasia/metabolism , Immunohistochemistry/methods , Liver/metabolism , Liver/pathology , Lung/metabolism , Lung/pathology , Lymph Nodes/metabolism , Lymph Nodes/pathology , Male , Microwaves , Neoplasms/pathology , Paraffin Embedding , Prostate/metabolism , Prostate/pathology , Skin/metabolism , Skin/pathology , Tongue/metabolism , Tongue/pathology , Urinary Bladder/metabolism , Urinary Bladder/pathology , tRNA Methyltransferases
11.
Minerva Urol Nefrol ; 49(4): 211-3, 1997 Dec.
Article in Italian | MEDLINE | ID: mdl-9557504

ABSTRACT

A case of well differentiated liposarcoma of the spermatic cord in a 80 year old man is presented. The preoperative diagnosis of spermatic cord liposarcoma is not easy; however, a careful comparison between clinical and ultrasonographic findings can lead to diagnostic suspicion. The treatment of choice is the excision of the liposarcoma associated with orchiectomy and high ligation of the spermatic cord to reduce the risk of recurrence. Postoperative radiotherapy may be indicated for poor differentiated liposarcomas.


Subject(s)
Genital Neoplasms, Male/pathology , Liposarcoma/pathology , Spermatic Cord , Aged , Aged, 80 and over , Genital Neoplasms, Male/surgery , Humans , Liposarcoma/diagnosis , Liposarcoma/surgery , Male , Spermatic Cord/pathology , Spermatic Cord/surgery
12.
Neuroreport ; 8(1): 45-8, 1996 Dec 20.
Article in English | MEDLINE | ID: mdl-9051750

ABSTRACT

Approximately 30% of patients with fatal head injuries have deposits of amyloid beta-protein (A beta); these are predominantly individuals carrying the epsilon 4 allele of apolipoprotein E (apoE). A beta deposition occurs in Alzheimer's disease (AD), for which approximately 50% of the genetic risk is attributed to apoE epsilon 4. The 1,1 genotype of a presenilin-1 (PS-1) polymorphism has been suggested to account for about half of the remaining genetic risk for AD. We related the PS-1 genotypes of 90 head-injured individuals to A beta deposition and apoE genotype. There was no difference in PS-1 genotype or allele frequencies between individuals with and without A beta deposits. Eighteen of 23 individuals with A beta deposits had apoE epsilon 4 as a risk factor. Three of five individuals without apoE epsilon 4 had the PS-1 1,1 genotype. If PS-1 genotype influences A beta deposition the effect is small and is overwhelmed by that of apoE genotype.


Subject(s)
Amyloid beta-Peptides/metabolism , Craniocerebral Trauma/metabolism , Membrane Proteins/genetics , Polymorphism, Genetic/physiology , Adolescent , Adult , Aged , Alleles , Apolipoproteins E/genetics , Apolipoproteins E/metabolism , Child , Child, Preschool , Genotype , Humans , Immunohistochemistry , Infant , Middle Aged , Presenilin-1
13.
Pathologica ; 88(4): 297-302, 1996 Aug.
Article in English | MEDLINE | ID: mdl-8956546

ABSTRACT

This study was performed on 1710 lung epithelial malignant tumours, diagnosed in the biennia 1963/1964 (42 cases), 1973/ 1974 (293), 1983/1984 (637) and 1993/1994 (738). It was aimed at evaluating whether, over a time span that long, the distribution of the histopathological patterns could have changed. The neoplasms were classified according to the WHO criteria (1981). From our data, a striking increase becomes apparent for all histotypes. Squamous cell carcinoma holds the leading position both among males and females, even though its rate of increase tends to slow down among males. Analogous trend is observed for all the other histotypes but adenocarcinoma. Among females, the rate of increase continues to accelerate for all histotypes but large cell carcinoma. The male to female ratio is ranging from 2:6 for adenocarcinomas to 12:1 for squamous cell carcinomas. In conclusion, impressive changes seem to have occurred in the frequency of the different lung oncotypes over this time span, especially for adenocarcinoma (increasing trend) and large cell carcinoma (decreasing trend). It also seems worth underlying that the marked increase among females could make lung carcinoma the number one cause of cancer-related deaths in this sex.


Subject(s)
Lung Neoplasms/pathology , Adenocarcinoma/pathology , Carcinoma, Large Cell/pathology , Carcinoma, Small Cell/pathology , Carcinoma, Squamous Cell/pathology , Female , Humans , Male , Middle Aged , Retrospective Studies , Sex Distribution
14.
Pathologica ; 87(6): 712-4, 1995 Dec.
Article in English | MEDLINE | ID: mdl-8927439

ABSTRACT

A rare case of breast carcinoma metastatising to ureter and presenting as urinoma is described. Reviewing the literature, only few cases of urinoma due to metastasis from breast carcinoma have to date been reported. The diagnostic role of immunohistochemical study in defining the disease is discussed.


Subject(s)
Breast Neoplasms/pathology , Carcinoma, Lobular/secondary , Ureteral Neoplasms/secondary , Ureteral Obstruction/etiology , Urine , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Breast Neoplasms/therapy , Carcinoma, Lobular/complications , Carcinoma, Lobular/diagnostic imaging , Carcinoma, Lobular/therapy , Combined Modality Therapy , Female , Humans , Middle Aged , Radiography , Ureteral Neoplasms/complications , Ureteral Neoplasms/diagnostic imaging , Ureteral Neoplasms/drug therapy , Ureteral Neoplasms/pathology
15.
Acta Cytol ; 39(4): 821-6, 1995.
Article in English | MEDLINE | ID: mdl-7631564

ABSTRACT

We describe a case of Acanthamoeba keratitis in a 20-year-old woman who wore disposable soft contact lenses. The diagnosis was made initially on the basis of a periodic acid-Schiff-stained corneal smear and subsequently confirmed by scanning electron microscopy and immunofluorescence. The patient was successfully treated with a combination of 0.053% polyhexamethylene biguanide and miconazole. Cytologic study and culture of corneal scrapings is relatively painless and inexpensive and may therefore be used for successful diagnosis and follow-up.


Subject(s)
Acanthamoeba Keratitis/diagnosis , Acanthamoeba/isolation & purification , Acanthamoeba/ultrastructure , Acanthamoeba Keratitis/drug therapy , Adult , Animals , Biguanides/therapeutic use , Contact Lenses , Cornea/parasitology , Cornea/ultrastructure , Female , Fluorescent Antibody Technique , Humans , Miconazole/therapeutic use , Microscopy, Electron, Scanning , Periodic Acid-Schiff Reaction
16.
Int J Gynecol Pathol ; 12(3): 259-63, 1993 Jul.
Article in English | MEDLINE | ID: mdl-8344763

ABSTRACT

Recent studies have shown that the silver-stained nucleolar organizer region (AgNOR) score is related to the cell growth rate in several neoplasms. In the work presented here, we tested the AgNOR technique in 79 ovarian epithelial tumors (13 benign, 10 borderline, 56 malignant) to evaluate the diagnostic potential of AgNOR count in distinguishing between ovarian borderline tumors and carcinomas and to assess its prognostic value in carcinomas. Ovarian carcinomas exhibited higher mean AgNOR values than borderline and benign tumors, but statistically significant differences were found only in the serous type. Statistical analysis showed a positive correlation of higher AgNOR score, advanced tumor stage, and adverse prognosis. On the contrary, low AgNOR counts identified stages I and II carcinomas with disease-free follow-up. These results suggest that the AgNOR count may improve the prognostic evaluation of ovarian epithelial tumors by representing a reliable indicator of survival.


Subject(s)
Nucleolus Organizer Region/pathology , Ovarian Neoplasms/pathology , Cell Division , Female , Humans , Neoplasm Recurrence, Local , Neoplasm Staging , Prognosis , Silver Staining
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