Bio-pump cadmium phytoextraction efficiency promoted by phytohormones in Festuca arundinacea.

The leaves of Festuca arundinacea can excrete cadmium (Cd) out onto the leaf surface, leading to a bio-pump phytoremediation strategy based on "root uptake-root-to-leaf translocation-leaf excretion". However, the bio-bump efficiency of soil Cd is a limiting factor for the implementation of this novel technology. Bio-bump remediation involves the bioprocess of plant root uptake from soil, root-to-leaf translocation, and leaf hydathode excretion. Here we show the significant effects of phytohormones in regulating the bio-pump phytoextraction efficiency. The results showed that salicylic acid and ethylene enhanced the whole process of Cd root uptake, root-to-leaf translocation, and leaf excretion, promoting the bio-pump phytoextraction efficiency by 63.6% and 73.8%, respectively. Gibberellin also greatly promoted Cd translocation, leaf excretion, and phytoextraction, but did not significantly impact Cd root uptake. Our results indicate that salicylic acid and ethylene could be recommended to promote bio-pump phytoextraction efficiency in F. arundinacea. Gibberellin might be used for a short-term promotion of the leaf Cd excretion.

Phytoextraction by harvesting dead leaves: cadmium accumulation associated with the leaf senescence in Festuca arundinacea Schreb.

Phytoextraction strategy by harvesting dead leaves provides continuous phytoremediation and a great saving in disposal cost of hazardous plant residues. This strategy is entirely dependent upon the amount of cadmium (Cd) accumulated in dead leaves. However, it is unknown that whether the leaf Cd accumulation is associated with its senescence and how to regulate its Cd accumulation. This study showed that Cd was preferentially and consistently distributed to and accumulated in the senescent leaves with the new leaf emergence and the old leaf dieback under 75 µM of Cd stress in tall fescue (Festuca arundinacea Schreb.). Individual leaf monitoring from its emergence to senescence showed that Cd concentration increased exponentially with the leaf life cycle, while leaf biomass decreased gradually after 14 days of leaf emergence. The total amount of Cd accumulated in the leaf showed an exponential increase during leaf senescence, regardless of the leaf biomass loss. Our results demonstrated that leaf Cd accumulation was significantly associated with its senescence and the highest Cd accumulated in dead leaves could be contributed from the continuous Cd input during the leaf senescent process, indicating that further regulatory studies should be focused on the leaf senescence process to achieve higher Cd accumulation and phytoextraction efficiency by harvesting dead leaves.

Cadmium binding during leaf senescence in Festuca arundinacea: Promotion phytoextraction efficiency by harvesting dead leaves.

Phytoextraction by harvesting dead leaves is a novel cadmium (Cd) phytoremediation strategy in tall fescue (Festuca arundinacea), which provides feasibility for the phytoremediation of Cd-polluted soils and cleaner food production. The highest Cd in dead leaves is the result of Cd accumulation during the process of leaf senescence. However, it is not known the mechanism of Cd accumulation during the leaf senescence, which limits the phytoextraction efficiency of this technology. In this study, we found that the contents of phytochelatins (PC), glutathione (GSH), and non-protein thiols (NPT) were increased during the process of leaf senescence and Cd stress significantly promoted PC, GSH, and NPT. Transcriptome analysis showed that the pathway of glutathione metabolism was significantly enriched in the senescent leaf under Cd stress. 19 genes encoding GST, enzymes catalyzing GSH-Cd binding, were up-regulated in the senescent leaf. The increases of PC, GSH, and NPT in the senescent leaf for Cd-binding could be from the pathways of the protein degradation rather than their synthesis, because genes encoding cysteine protease (catalyzes protein degradation) were significantly promoted, but both GSH synthetase (GS) and PC synthetase (PCS) did not show the significant changes between the young and senescent leaves. Our results indicated that Cd accumulation during the leaf senescence could be the result of the promotion of Cd-binding by PC, GSH, and NPT, which provide insights into the regulatory mechanism and further genetic engineering to promote the phytoextraction efficiency by harvesting dead leaves in tall fescue.

Zinc promotes cadmium leaf excretion and translocation in tall fescue (Festuca arundinacea).

Phytoexcretion is a novel strategy to remediate cadmium (Cd) pollution by leaf excretion in tall fescue (Festuca arundinacea), which involves the processes of Cd leaf excretion, root-to-leaf translocation, and root uptake. A hydroponic experiment was designed to investigate a series of 11 zinc (Zn) concentrations on Cd leaf excretion in tall fescue under 75 µM Cd stress. The results showed that the promotions of Zn on Cd leaf excretion, root-to-leaf translocation, and leaf accumulation were concentration-dependent in tall fescue. Zn treatments at 90 and 135 µM resulted in the highest Cd leaf excretion with 118.1 and 123.6 mg/kg of Cd excretion amount and 27.0 and 26.6% of excretion ratio, which were 2.6 and 2.7 fold of the control (15 µM of Zn), respectively. Cd leaf excretion was decreased when Zn treatments reached 180 µM, which could be toxic to plants as indicated by the decline of plant biomass. Zn also promoted leaf Cd accumulation and Cd translocation from roots to leaves and reached the highest at 90 and 180 µM respectively. Root Cd accumulation decreased with the increase of Zn concentrations, but the total plant Cd uptake did not decrease significantly until Zn concentration reached 90 µM. Our results indicate that 90 µM of Zn treatment can be served as the threshold to promote Cd leaf excretion and improve the efficiency of Cd phytoexcretion in tall fescue.