ABSTRACT
AIM: Study the effect of single nucleotide polymorphism genes TP53 (rs1042522, rs1800371), CDKN2A (rs3731217, rs3088440) and MDM2 (rs2279744) on the risk of development of color- ectal cancer (CRC) in population of Perm Region. MATERIALS AND METHODS: Case group con- sisted of 198 patients with histologically verified CRC, control group - 205 individuals with CRC excluded by results of colonoscopy. DNA genotyping, obtained from leukocytes of venous blood ofthe studied individuals; was carried out by PCRwith electrophoretic detection of results. RESULTS: Significant inter-population differences of frequency of occurrence of alleles rs 1042522; rs3088440, rs2279744 in Russian population compared with East-Asian and European were detected (p<0.0001). Association of heterozygote (G/T) genotype rs2279744 with a lower risk of development of CRC regardless of sex and age (OR=0.51, 95% CI=0.26 - 0.97) was established. Statistically significant relations between development of CRC and other polymorphisms were not determined. CONCLUSION: Relations of gene polymorphism of apoptosis system with risk of development of CRC in Russian population was studied for the first time. The data obtained give evidence on the probable reduction of risk of development of CRC with carriage of hetero- zygote genotype of polymorphism rs2279744.
Subject(s)
Apoptosis/genetics , Colorectal Neoplasms/genetics , Cyclin-Dependent Kinase Inhibitor p18/genetics , Polymorphism, Single Nucleotide , Proto-Oncogene Proteins c-mdm2/genetics , Tumor Suppressor Protein p53/genetics , Age Factors , Aged , Colorectal Neoplasms/epidemiology , Cyclin-Dependent Kinase Inhibitor p16 , Female , Humans , Male , Middle Aged , Risk Factors , Russia/epidemiology , Sex FactorsABSTRACT
The article presents the results of an epidemiological descriptive and retrospective study of the malignant tumor incidence in the Perm region according to official statistics for the 2002-2014. Through the use of standardized rates it was revealed that the incidence of growth mainly due to an increased risk of illness because of the intensification of epidemiological factors (13,1%). Changes in the total extent and structure of population resulted in growth of number of cases by 3,5% of the total increase. Over 80% of the total incidence was recorded among persons older than 50 years. Cancer of the breast, skin, colon and rectum and lung were characterized by the greatest intensity of oncoepidemiological process. The highest growth rate of incidence had ovarian cancer, cervical cancer, endometrial cancer and prostate cancer.
Subject(s)
Neoplasms/epidemiology , Adult , Age Factors , Aged , Animals , Female , Humans , Incidence , Male , Middle Aged , Retrospective Studies , Russia/epidemiologyABSTRACT
In the present work, a total of 132 HIV-1 env gene C2-V3-C3 sequences belonging to the IDU-A genetic variant were analyzed. The variants were obtained from the viruses circulating among IDUs and heterosexuals in the Perm region at different periods. It was shown that the rate of the divergence of the IDU-A HIV-1 viruses from a common ancestor increased 4.3 times (p < 0.001) in 2011 as compared with the onset of the epidemics. The rate of the HIV-1 evolution was different in the two risk groups of the infection. The mean genetic distance of HIV-1 variants circulating among heterosexuals was 1.3 times longer (p = 0.008) than that among IDUs. The accumulation rate of the nucleotide (including nonsynonymous) substitutions in the C2-V3-C3 HIV-1 env gene region among individuals infected by heterosexual contacts was 1.7 times higher than that among IDUs. The differences in the positions of the codons subjected to positive selection were demonstrated depending on the infection risk group tested.
