[The immunophenotypic and cytogenetic characteristics of the blast cells in subvariants of acute myeloid leukemia]. / Immunofenotipicheskaia i tsitogeneticheskaia kharakteristika blastnykh kletok pri nekotorykh podvariantakh ostroi mieloidnoi leikemii.

The results of study of morpho-cytochemical peculiarities, antigenic profile and peripheric blood and/or bone marrow blast cell karyotype of 21 patients with acute myeloid leukemia are presented. Hemopoietic cell immunophenotyping was carried out with the use of cytofluorometer FACScan and chromosome cytogenetic analysis with the use of analyzer "Metascan". It has been shown that in the AML M1 blast cell plasmatic membrane carries pan-myeloid CD33 and CD13 antigens, the last having high density of expression, and the CD38 antigen, which is a myeloid cell-precursor marker. In these patients tetraploidy, being the testimony of karyotype change, has been ascertained. It has been found out that the AML M2 blasts, except pan-myeloid antigens, express the cell proliferation CD71 marker. Blast cell karyotype peculiarities typical for this leukemia sub-variant have been revealed. In patients with the AML M4 in 3 of 6 cases an anomalous karyotype has been found. It has been also shown that the CD14 antigen, and rather its percentage in blast total population, is the differential-diagnostic criterion for the AML M4 and M5.

[An assessment of liver function in victims as a result of the accident at the Chernobyl Atomic Electric Power Station]. / Otsenka funktsional'nogo sostoianiia pecheni u lits, postradavshikh v resul'tate avarii na ChAES.

A total of 254 Kievites of both sexes 19 to 35 years old were examined over 1987-1990. Revealed in the above population were alterations of the functional state of the liver characterizing the amino acid, lipid, and nucleotide metabolisms and suggesting impaired permeability of hepatocytic membranes, inadequate energy security of hepatic cells, and indicating the intrahepatic circulation state and bodily immune reactivity.

[The efficacy of using thymalin and plasmapheresis in the combined treatment of patients with chronic lympholeukemia]. / Effektivnost' primeneniia timalina i plazmafereza v kompleksnom lechenii bol'nykh khronicheskim limfoleikozom.

Results are submitted of study of the effectiveness of inclusion into the treatment program for patients with chronic lymphoid leukemia of specific (thymalin) and unspecific (plasmapheresis) means and modes of immune correction, which made for more rapid attaining clinical and hematological compensation by comparison with chemotherapeutic agents only. Furthermore, the method of combined use of drugs, attempted for the first time, permitted the functional activity of the lymphoid system to be improved, with the clinical and hematological compensation being achieved in a shorter space of time.

[The criteria of disorders in body antitumor resistance in subjects with dyshemopoiesis]. / Kriterii narushenii protivoopukholevoi rezistentnosti organizma u lits s disgemopoézom.

A complex of methods is proposed adapted to practices common in clinical and diagnostic laboratories that enables the state of bodily antitumoral resistance to be properly assessed. A diagnostic system of disturbances in the antineoplastic resistance of the organism, which makes for detection of endogenous factors marking a high risk for the development of oncohemopathology is suggested.

[The efficacy of active specific immunotherapy using modified neoplastic cells in the combined modality treatment of hemoblastoses]. / Effektivnost' aktivnoi spetsificheskoi immunoterapii modifitsirovannymi neoplasticheskimi kletkami v kompleksnom lechenii gemoblastozov.

Efficiency was studied of active specific immunotherapy (IT) with cell vaccine consisting of allogenic viable cryopreserved modified Con A blast cells identical with the illness in patients with acute myeloblastic leukemia and acute lymphoblastic leukemia. The studies made confirmed a positive impact of IT on the clinical course of acute leukosis (prolonged remission, keeping down infectious complications), on leukopoiesis, peripheral blood cells enzymatic status as well as on cell immunity, certain factors of humoral immunity and bodily natural antineoplastic resistance.

[The cytomorphological characteristics of the hemopoietic cells in a preleukemic state]. / Tsitomorfologicheskaia kharakteristika gemopoéticheskikh kletok pri predleikoznom sostoianii.

The peripheral blood from 30 patients presenting with preleukosis states was studied. Both quantitative and qualitative indices of blood and bone marrow were determined. It has been ascertained that the following signs should be taken in consideration where the diagnosis is "preleukosis": anemia presenting with qualitative changes in erythron, neutrophylopenia presenting with a shift to the left, thrombocytopenia, nuclear anomalies of neutrophylocytes and lymphocytes (pelgeroides, fragmentation, pyknosis, Rider's nuclei).

[Homeostatic indices in persons subjected to ionizing radiation exposure]. / Nekotorye pokazateli gomeostaza u lits, podvergshikhsia vozdeistviiu ioniziruiushchego izlucheniia.

Over the period of 1986-1990 a comprehensive evaluation was carried out in a series of a 5578 subjects exposed to ionizing radiation because of Chernobyl NPP breakdown, using hematologic, immunologic and biochemical methods of investigation. The above series included liquidators of aftermath of the accident, residents of Pripyat' and Kiev. Changes were revealed in all the parameters studied. In view of the abnormal changes in the values for blood, immunologic status and biochemical tests characterizing the functional state of the liver in those subjects having been in the area of abnormal radiation, as well as its potential remote sequelae, it is advisable that the above contingents of the population be under prophylactic management.

[Immunological deficiency in patients with the myeloid forms of acute leukemia and the methods for its correction]. / Immunologicheskaia nedostatochnost' u bol'nykh s mieloidnymi formami ostrogo leikoza i metody ee korrektsii.

Immunologic and cytochemical studies were done in 162 patients with myeloid forms of acute leukosis, among whom 130 had acute myeloblastic leukemia (AML), and thirty two--and acute myelomonoblastic leukemia (AMML), before and after the therapy instituted. Immunocorrection was attempted with immunomodulating agents prodigiosane and tactivin. Striking inhibition of T-cellular immunity (T-total, T-active, TPhres, TPhsen, as well as blast transformation of lymphocytes with PHA) was revealed. All patients experienced significant rise in circulating immune complexes before treatment. Investigation designed to study intracellular metabolism of neutrophils demonstrated significant decline in the activities of G-6-P-ase, NAD- and NADP-oxidases, cationic proteins and NBT-test. Immunocorrective agents improve the state of immunologic reactivity, make for arresting infections and inflammatory processes and prolongation of remission in patients with acute myeloid forms of leukoses.

[Experimental substantiation of the possibility to obtain anti-Proteus plasma]. / Eksperymental'ne obhruntuvannia mozhlivosti oderzhannia anty- proteinoï plazmy krovi.

The possibility to produce the anti-Proteus plasma is experimentally substantiated. It is determined that polyvalent Proteus antigen immunization permits producing blood immune preparation, that is the high-active anti-Proteus plasma. its antibodies belonging to the class of immunoglobulin G. The anti-Proteus plasma in liquid (at t = 6 degrees divided by 2 degrees C) and in frozen (at t = 20 degrees-5 degrees C) forms retains the specific activity for 3 days and 6 months, respectively.

[The results of studies of the peripheral blood from children in Polesie District, Kiev Province and in the city of Kiev 4 years after the accident at the Chernobyl Atomic Electric Power Station]. / Rezul'taty issledovanii perifericheskoi krovi detei Polesskogo raiona Kievskoi oblasti i g. Kieva cherez chetyre goda posle avarii na Chernobyl'skoi AES.

The peripheral blood of 2200 children (age: from 2 to 16 years) inhabiting the Polessye District of Kiev Province and the city of Kiev 4 years after the Chernobyl atomic station accident was studied. An analysis of results revealed the presence of hematologic changes that may be interpreted as sequels of the effect of ionizing radiation on the body, while other changes may be considered as reactive and may in most cases accompany different somatic diseases of infectious-inflammatory character.

[Large granule-containing lymphocytes in patients with infectious mononucleosis]. / Bol'shie granulosoderzhashchie limfotsity u bol'nykh infektsionnym mononukleozom.

A study of 50 children with infectious mononucleosis is presented. Results indicate that the content of large granule-containing lymphocytes corresponds to the severity of the clinical course of infectious mononucleosis. Absence of large granule-containing lymphocytes evidences severe course of the disease while their appearance favoured normal reconvalescence of patients. A high content of large granule-containing lymphocytes is an evidence of a favourable prognosis of the course of infectious mononucleosis.

[The function of the T immunity system in patients with hemoblastoses undergoing polychemotherapy]. / Sostoianie T-sistemy immuniteta u bol'nykh gemoblastozami pri polikhimioterapii.

The theophylline test was used to evaluate the state of T-system immunity in patients with different variants of acute leucosis and in mice with transplantable NK/lymphoma against the background of chemo- and immunotherapy. Analysis of cellular immunoregulatory subpopulations and comparison with the frequency and character of inverse effect of theophylline on the reaction of E-rosette formation showed high informative value and prognostic significance of the theophylline test in patients with hemoblastoses.

[The functional activity of the natural killers in patients with infectious mononucleosis and other viral infections]. / Funktsional'naia aktivnost' estestvennykh killerov u bol'nykh infektsionnym mononukleozom i drugimi virusnymi infektsiiami.

The natural killer activity (NKA) and content of large granule-containing lymphocytes (LGL) were determined in 50 patients with infectious mononucleosis and in 36 patients with other viral infections. Results indicate that in 27 of 50 patients with infectious mononucleosis the number of LGL was less than 5% or equalled O. In 12 patients with infectious mononucleosis the number of LGL was 5% and in 11 children it was 5-20%. In children with different viral infections not related to the Epstein-Barr virus the number of LGL was below normal values. The NKA corresponded to the quantitative indices of LGL as well as the severity of the clinical condition.

[Characteristics of the immune status of patients with hemophilia]. / Osobennosti immunnogo statusa bol'nykh gemofiliei.

In examination of 72 patients with hemophilia before and after the orthopedic operations, the increase in the content of the T- and B-lymphocytes, circulating immune complexes, and reduction in the number of the large granule-containing lymphocytes were established.

[Unresolved questions on the diagnosis of hepatitis in donors]. / Nekotorye nereshennye voprosy diagnostiki gepatitov u donorov.

Clinico-laboratory characteristics of liver lesions occurring in 64 immune donors of blood plasma of 0(1) group are presented. The diseases ran as subclinical, latent, anicteric forms of hepatitis, and only in 5 persons as acute icteric hepatitis. In 50% of the patients there were signs of the disease becoming chronic: long-term increase in the blood serum of the activity of alanine aminotransferase, alkaline phosphatase, variations in bilirubin levels. Certain unresolved and disputable problems of etiological diagnosis of hepatitis in plasma donors, especially in differentiation of autoimmune hepatitis and non-A-non-B hepatitis are discussed.