ABSTRACT
Bone Marrow Transplant (BMT) is a lifesaving procedure for several diseases. Histocompatibility between host and donor is crucial for the success of this therapy. Since human leukocyte antigen (HLA) genes are the most polymorphic in humans, a foreknowledge of their frequencies and a vast register of volunteer donors are important for patients who need an unrelated donor. This research evaluated data from 3500 HLA-typed donors using PCR-SSP (One Lambda ABDRX kits, low/medium resolution). The allele and haplotype frequencies were calculated from the donor HLA typings. Among the total sample we observed that the allelic groups HLA-A*02, -B*35, and -DRB1*11 had frequencies of 0.228, 0.112, and 0.125, respectively. The haplotype HLA-A*01-B*08-DRB1*03 was the most frequent (0.024). We also compared our results with another study conducted in a Northeastern Brazilian population. The identification of the most common allelic groups and haplotypes is of great interest, not only to know the HLA composition of the population but also to search for the best HLA match for a transplant. However, the search for the perfect match depends on the eventual registration of every specific donor in the national registers for bone marrow donation.
Subject(s)
Bone Marrow Transplantation/immunology , HLA Antigens/genetics , HLA Antigens/immunology , Haplotypes , Living Donors , Brazil , HLA-B Antigens/genetics , HLA-B Antigens/immunology , HLA-DR Antigens/genetics , HLA-DR Antigens/immunology , HLA-DRB1 Chains , Histocompatibility Testing , Humans , Polymerase Chain Reaction , Polymorphism, Single-Stranded ConformationalABSTRACT
PIP: Findings are reported from a study conducted to fully sequence the gp120 gene from a Brazilian HIV-1 isolate containing the GWGR motif and compare it to the Brazilian B and F sequences already described. Genomic DNA isolated from six patients in an ongoing HIV cohort study was screened for the presence of the viral V3 loop GWGR motif. Sequence analysis revealed that BZ(GWGR)1 is closely related to the North American MN prototype strain, with 80.1% amino acid identity and 89.1% nucleic acid similarity, and with 6 deletions and 11 insertions. Large differences were, however, observed when the V1 and V2 regions of MN and BZ(GWGR)1 were compared. Tree analysis based upon amino acid sequences and the four Brazilian isolates introduced in the analysis indicate that BZ(GWGR)1 belongs to the HIV-1 B subtypes. Several features of BZ(GWGR)1 suggest that some biological advantage may be derived from the differences between that variant and the American/European prototype strain.^ieng
