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1.
J Virol Methods ; 175(1): 109-16, 2011 Jul.
Article in English | MEDLINE | ID: mdl-21569796

ABSTRACT

The non-structural 1 (NS1) protein plays an important role in dengue diagnosis because it has been detected as a soluble serum antigen in both primary and secondary infections. The NS1 protein was expressed in Escherichia coli cells, and the efficiency of four different refolding protocols was tested. All of the protocols generated dimeric NS1 in a conformation similar to that of the protein expressed by eukaryotic cells. A polyclonal antibody produced from the properly folded E. coli recombinant NS1 (rNS1) protein proved to be a useful tool for the diagnosis of Dengue virus because it detected 100% of the Dengue virus 2 (DENV2) in infected patients' sera and 60% of the DENV IgM-positive sera not detected by commercial NS1-based diagnostic kits. These data suggest a high-efficiency method for correctly folding rNS1 that maintains its structural and immunogenic properties. In addition, a detection method using the polyclonal antibody against correctly folded rNS1 seemed to be more sensitive and efficient for NS1 detection in serum, highlighting its usefulness for developing a high-sensitivity diagnostic kit.


Subject(s)
Antibodies, Viral/immunology , Dengue Virus/immunology , Dengue/diagnosis , Escherichia coli/metabolism , Protein Folding , Viral Nonstructural Proteins/immunology , Animals , Antibodies, Viral/blood , Blotting, Western , Dengue Virus/genetics , Early Diagnosis , Enzyme-Linked Immunosorbent Assay/methods , Escherichia coli/genetics , Glycoproteins/immunology , Humans , Mice , Mice, Inbred BALB C , Rabbits , Viral Nonstructural Proteins/genetics , Viral Nonstructural Proteins/metabolism
2.
J Virol Methods ; 167(2): 186-92, 2010 Aug.
Article in English | MEDLINE | ID: mdl-20399232

ABSTRACT

The dengue virus NS1 protein has been shown to be a protective antigen under different experimental conditions but the recombinant protein produced in bacterial expression systems is usually not soluble and loses structural and immunological features of the native viral protein. In the present study, experimental conditions leading to purification and refolding of the recombinant dengue virus type 2 (DENV-2) NS1 protein expressed in Escherichia coli are described. The refolded recombinant protein was recovered as heat-stable soluble dimers with preserved structural features, as demonstrated by spectroscopic methods. In addition, antibodies against epitopes of the NS1 protein expressed in eukaryotic cells recognized the refolded protein expressed in E. coli but not the denatured form or the same protein submitted to a different refolding condition. Collectively, the results demonstrate that the recombinant NS1 protein preserved important conformation and antigenic determinants of the native virus protein and represents a valuable reagent either for the development of vaccines or for diagnostic methods.


Subject(s)
Dengue Virus/chemistry , Dengue Virus/immunology , Protein Folding , Viral Nonstructural Proteins/chemistry , Viral Nonstructural Proteins/immunology , Antibodies, Viral/immunology , Dengue Virus/genetics , Escherichia coli/genetics , Gene Expression , Humans , Protein Multimerization , Protein Stability , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Spectrum Analysis , Temperature , Viral Nonstructural Proteins/genetics
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