An ultrasonic-based experimental model to evaluate the setting time of endodontic sealers.

AIM: To evaluate an ultrasonic-based experimental model (US), to assess the setting time of AH Plus® , Bio-C Sealer® and MTA Fillapex® , and compare the results obtained with ANSI/ADA specifications (2012). METHODS: To determine the setting time according to the ANSI/ADA specification, moulds measuring 10 mm (diameter) × 2 mm (thickness) were used, and for the US tests a transducer (1 MHz) and an oscilloscope were used. Fourier-transform infrared spectroscopy (FTIR) was used to evaluate the changes on chemical structure at the different setting times found by the US and ANSI/ADA methods. After checking the normal distribution, setting time data were analysed using unilateral analysis of variance with Tukey-HSD post-test to compare the methods. RESULTS: AH Plus® and Bio-C Sealer® had longer mean setting time values for the US method than for the ANSI/ADA (p < .05), whilst for MTA Fillapex® no significant difference was found between the methods (p < .05). FTIR spectra demonstrated that at the setting time determined by the US method, AH Plus® and Bio-C Sealer® were in a more advanced stage of polymerization than at the ANSI/ADA, whilst there was no significant difference for MTA Fillapex® . CONCLUSIONS: The use of US was effective for setting time determination and measured longer values than ANSI/ADA specification for AH Plus® and Bio-C Sealer® , identifying the entire (and not only superficial) setting of the endodontic sealers.

Influence of endodontic sealers with different chemical compositions on bond strength of the resin cement/glass fiber post junction to root dentin.

OBJECTIVE: This study evaluated the influence of endodontic sealers with different chemical compositions on bond strength of resin cement/glass fiber post junction to root dentin. MATERIALS AND METHODS: Seventy-two maxillary canines roots were instrumented with Reciproc (#R50) and distributed in 6 groups (n = 12) according to endodontic sealers: G1-control (no sealer); G2-Endofill; G3-Sealapex; G4-AH Plus; G5-Sealer Plus; G6-EndoSequence BC. The glass fiber post was cemented with Panavia F in 10 of the specimens. Two specimens from each group did not receive post and were analyzed by SEM to verify presence of filling material. Three slices were obtained from each third. The first slice was subjected to push-out test and type of failure. The second slice was imaged using laser confocal microscopy for evaluation of dentin/resin cement adhesive interface. Bond strength (BS) data were analyzed using two-way ANOVA and Tukey's post hoc tests; for LCM data, we used Kruskal-Wallis and Dunn's tests. RESULTS: Endofill yielded the lowest BS values (p < 0.05). The control and AH Plus groups differed from all others (p < 0.05). In all groups, adhesive to dentin failure predominated. Most specimens of control and AH Plus groups had acceptable interfaces, while those of EndoSequence BC, Sealapex, and Sealer Plus groups ranged between acceptable and poor interfaces. Overall, few filling material remnants were observed on the dentin. CONCLUSION: EndoSequence BC, Sealapex, Sealer Plus, and Endofill negatively interfere with BS of glass fiber post/resin cement junction to root dentin. CLINICAL RELEVANCE: In cases whose restoration planning includes a post cemented with resin cement, it is recommended that endodontic sealer be based on epoxy resin.

Analysis of the shelf life of chitosan stored in different types of packaging, using colorimetry and dentin microhardness.

OBJECTIVES: Chitosan has been widely investigated and used. However, the literature does not refer to the shelf life of this solution. This study evaluated, through the colorimetric titration technique and an analysis of dentin micro-hardness, the shelf life of 0.2% chitosan solution. MATERIALS AND METHODS: Thirty human canines were sectioned, and specimens were obtained from the second and third slices, from cemento-enamel junction to the apex. A 0.2% chitosan solution was prepared and distributed in 3 identical glass bottles (v1, v2, and v3) and 3 plastic bottles (p1, p2, and p3). At 0, 7, 15, 30, 45, 60, 90, 120, 150, and 180 days, the specimens were immersed in each solution for 5 minutes (n = 3 each). The chelating effect of the solution was assessed by micro-hardness and colorimetric analysis of the dentin specimens. 17% EDTA and distilled water were used as controls. Data were analyzed statistically by two-way and Tukey-Kramer multiple comparison (α = 0.05). RESULTS: There was no statistically significant difference among the solutions with respect to the study time (p = 0.113) and micro-hardness/time interaction (p = 0.329). Chitosan solutions and EDTA reduced the micro-hardness in a similar manner and differed significantly from the control group (p < 0.001). Chitosan solutions chelated calcium ions throughout the entire experiment. CONCLUSIONS: Regardless of the storage form, chitosan demonstrates a chelating property for a minimum period of 6 months.

Evaluation of the chelating effect of chitosan solubilized in different acids.

AIM: This study aimed to compare, through dentin microhardness and colorimetric analysis, the chelating effect of 0.2% chitosan solubilized in different acids. MATERIALS AND METHODS: The second and third cuts of the cervical region of maxillary central incisors were divided into four quadrants, resulting in eight specimens, which were treated with 50 µL of solution for 5 min according to their group (n = 10): GI - 0.2% chitosan solubilized in 1% acetic acid; GII - 0.2% chitosan solubilized in 3.3% citric acid; GIII - 0.2% chitosan solubilized in 0.00145% hydrochloric acid; and GIV - 0.2% chitosan solubilized in 0.00112% nitric acid. A control was made from the chelating properties of the following acids: GV - 3.3% citric acid, GVI - 0.00145% hydrochloric acid, GVII - 0.00112% nitric acid, and GVIII - control (distilled water). Afterward, they were subjected to the Knoop microhardness tester with a load of 10 g for 15 s, resulting in three indentations of the root canal toward the cement. The measurements obtained were subjected to the one-way ANOVA test followed by Tukey's test (α =0.05). Subsequently dispensing the chitosan solutions, the same were subjected to colorimetric analysis. RESULTS: Chitosan solubilized in acetic acid, followed by chitosan in citric acid, provided a greater reducing effect compared to the other groups. Similar results were observed in the colorimetric analysis. CONCLUSION: It was concluded that the chelating ability of the chitosan solution solubilized in acetic acid is higher than solubilization in citric, hydrochloric, and nitric acids.

Time-dependent effects of chitosan on dentin structures.

Complete debridement with smear layer removal are essential measures for achieving a successful outcome of root canal treatment. The aim of this study was to evaluate the effects of chitosan at different concentrations on the removal of the smear layer and on dentin structure after 3 and 5 min of application. Twelve recently extracted maxillary canine teeth were instrumented using the crown-down technique and irrigated with 1% sodium hypochlorite. The specimens were distributed according to the time and concentration of the final irrigating solution: G1: 0.1% chitosan for 3 min; G2: 0.2% chitosan for 3 min; G3: 0.37% chitosan for 3 min; G4: 0.1% chitosan for 5 min; G5: 0.2% chitosan for 5 min; G6: 0.37% chitosan for 5 min. All samples were prepared for SEM analysis. G1 exhibited removal of the smear layer, but not the smear plugs. G2 showed visible and open tubules with slight erosion of the peritubular dentin. Cleaning in G3 was similar to that in G2, however, the erosive effect was greater. There was expansion of the diameter of the tubules in G4; and in G5 and G6, there was severe erosion with deterioration of dentin surface. In conclusion, 0.2% chitosan for 3 min appeared to be efficient for removing the smear layer, causing little erosion of dentin.

Conventional Treatment of Maxillary Incisor Type III Dens Invaginatus with Periapical Lesion: A Case Report.

Dens invaginatus is a developmental dental anomaly clinically characterized by a palatine furrow that can be limited to the coronal pulp or may extend to the radicular apex. The purpose of this paper was to present a clinical case of type III dens invaginatus, identified on the maxillary right central incisor in anterior periapical radiographs, in which the tooth was submitted to conventional endodontic treatment. The results obtained after five years of clinical and radiographic followup demonstrated that conventional endodontic treatment is a clinically viable alternative in cases of type III dens invaginatus.

Temperature variation on the external root surface during intracanal Er:YAG laser irradiation.

OBJECTIVE: To evaluate the temperature changes on the root's external surface during Er:YAG laser irradiation with different tips and pulse repetition rates. BACKGROUND DATA: There have been limited reports that correlate temperature variations with Er:YAG laser irradiation using different tips and pulse repetition rates. MATERIALS AND METHODS: Sixty roots of central incisors were sectioned 10 mm from the apex, biomechanically prepared, embedded in acrylic resin, and randomly distributed into six groups: the teeth in group I, group II, and group III were irradiated with an Opus 20 laser with a sapphire tip at 8, 10, and 15 Hz, respectively; the teeth in group IV, group V, and group VI were irradiated with the fiberoptic tip of a Kavo Key laser at 6, 10, and 15 Hz, respectively. Laser irradiation was applied for 20 sec to all teeth and temperature changes were recorded with thermocouples in the root surfaces. RESULTS: There were significant differences (p < 0.05) among pulse repetition rates: 6 and 8 Hz (2.36 degrees +/- 0.91 degrees C), 10 Hz (4.92 degrees +/- 1.60 degrees C), and 15 Hz (8.23 degrees +/- 2.78 degrees C); and radicular thirds: apical (8.33 degrees +/- 2.69 degrees C), middle (4.70 degrees +/- 1.60 degrees C), and cervical (2.48 degrees +/- 0.83 degrees C). No significant differences were seen between the two types of laser tips used (p > 0.05). CONCLUSION: The temperature changes on root external surfaces remained above the critical threshold (13 degrees C) during Er:YAG laser irradiation, except for irradiation with 15 Hz in the apical third. However, the type of Er:YAG laser tip used (sapphire or fiberoptic) did not affect the temperature variation.

Morphometric analysis of the effectiveness of different concentrations of sodium hypochlorite associated with rotary instrumentation for root canal cleaning.

This study evaluated the cleaning capacity of Profile.04 instruments with different concentrations of NaOCl solutions on flattened root canals. Twenty central mandibular incisors were divided into 4 groups: Group I: 0.5% NaOCl; Group II: 1% NaOCl; Group III: 5% NaOCl; Group IV: distilled and deionized water. After chemomechanical preparation, the teeth were submitted to histological preparation and morphometric analysis. Morphometric analysis showed that the 5% NaOCl was significantly more efficient in cleaning the root canal than 0.5% NaOCl and distilled water, which had the lowest levels of cleaning of the root canals (p<0.01).

Histologic analysis of the cleaning capacity of mechanical endodontic instruments activated by the ENDOflash system.

The cleaning capacity of mechanical endodontic instruments activated by the ENDOflash system was evaluated by means of histologic and morphometric analyses. Twenty-two distobuccal roots of human maxillary molars from laboratory stock were randomly separated into four groups. Group 1 was instrumented with ENDOflash files, group 2 with ProFile taper .04 files, group 3 received instrumentation with Pow-R taper .04 files, and group 4 (positive control) was instrumented with Nitiflex files. After instrumentation, teeth were sectioned transversally and processed for microscopic evaluation to determine the amount of debris on root canal walls. Results obtained by morphometric analysis indicated significant statistical differences (p < 0.01) between groups. ProFile taper .04 files were significantly more efficient in cleaning the root canal, followed by Pow-R files, Nitiflex, and ENDOflash, which had the lowest levels for cleaning the root canals.