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Accuracy of PCR universal primer for methicillin-resistant Staphylococcus and comparison of different phenotypic screening assays.

de Melo, Dayanne A; da S Soares, Bianca; da Motta, Cássia C; Dubenczuck, Felipe C; Barbieri, Nicolle L; Logue, Catherine M; de O Coelho, Shana; da S Coelho, Irene; de Souza, Miliane M S.

Braz J Microbiol ; 51(1): 403-407, 2020 Mar.

Article in English | MEDLINE | ID: mdl-31664699

ABSTRACT

Characterization of methicillin-resistant Staphylococcus (MRS) is a continuous challenge at diagnostic laboratories. The phenotypic methods present heterogeneous results and the occurrence of variants of mecA gene turned this goal even more challenging to achieve. The present study provided an accurate and highly discriminatory screening tool for MRS, improving its detection.

Subject(s)

Bacterial Proteins/genetics , Methicillin Resistance/genetics , Methicillin-Resistant Staphylococcus aureus/isolation & purification , Penicillin-Binding Proteins/genetics , Polymerase Chain Reaction/methods , Animals , Anti-Bacterial Agents/pharmacology , Cattle/microbiology , DNA Primers , Genes, Bacterial , Genetic Variation , Humans , Methicillin Resistance/drug effects , Methicillin-Resistant Staphylococcus aureus/drug effects , Methicillin-Resistant Staphylococcus aureus/genetics , Microbial Sensitivity Tests , Pets/microbiology , Pork Meat/microbiology , Poultry/microbiology , RNA, Ribosomal, 16S , Red Meat/microbiology , Sheep/microbiology , Staphylococcal Infections/microbiology , Staphylococcal Infections/veterinary

ABSTRACT

Subject(s)

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