ABSTRACT
Papillomaviruses (PVs) are circular double-stranded DNA virus belonging to Papillomaviridae family. During the infection cycle, PVs translate proteins that can influence cell growth and differentiation, leading to epidermal hyperplasia and papillomas (warts) or malignant neoplasms. Canis familiaris papillomaviruses (CPVs) have been associated with different lesions, such as oral and cutaneous papillomatosis, pigmented plaques, and squamous cell carcinomas (SCCs). Here, we report a clinical case of a mixed bred female dog with pigmented plaques induced by CPV16 (Chipapillomavirus 2) that progressed to in situ and invasive SCCs. Gross and histological findings were characterized, and the lesions were mainly observed in ventral abdominal region and medial face of the limbs. In situ hybridization (ISH) revealed strong nuclear hybridization signals in the neoplastic epithelial cells, as well as in the keratinocytes and koilocytes of the pigmented viral plaques. The full genome of the CPV16 recovered directly from the lesions was characterized, and the phylogenetic relationships were determined. The identification of oncoprotein genes (E5, E6, and E7) by high throughput sequencing (HTS) and their expected domains are suggestive of the malignant transformation by CPV16.
Subject(s)
Carcinoma, Squamous Cell/veterinary , Neoplasms/veterinary , Papillomavirus Infections/veterinary , Parvovirus, Canine/pathogenicity , Skin Neoplasms/veterinary , Animals , Carcinoma, Squamous Cell/virology , DNA, Viral/genetics , Dog Diseases/virology , Dogs , Female , Genome, Viral , Neoplasms/virology , Papillomavirus Infections/complications , Parvovirus, Canine/genetics , Phylogeny , Skin/pathology , Skin/virology , Skin Neoplasms/virologyABSTRACT
Bovine papillomaviruses (BPVs) have been described as etiologic agents of cutaneous and mucosal papillomas in cattle. In the present study, we describe a new BPV that was detected in a cutaneous papilloma from a cow. Phylogenetic analysis suggests that this virus belong to the genus Xipapillomavirus, and we refer to it here as BPV type 24 (BPV24). Coinfection with members of the genera Epsilonpapillomavirus and Deltapapillomavirus in a cutaneous papilloma from a different animal was also detected, and the full genomes of these viruses were sequenced. Both papillomas were from cattle within Acre State in the Amazon region of Brazil. The data presented here demonstrate the utility of using high-throughput methods to indentify coinfections and allow the characterization of new genomes.
Subject(s)
Cattle Diseases/virology , Papillomavirus Infections/veterinary , Xipapillomavirus/isolation & purification , Animals , Base Sequence , Brazil , Cattle , Genome, Viral , Molecular Sequence Data , Open Reading Frames , Papillomavirus Infections/virology , Phylogeny , Xipapillomavirus/classification , Xipapillomavirus/geneticsABSTRACT
Papillomaviruses are small and complex viruses with circular DNA genome that belongs to the Papillomavirus family, which comprises at least 39 genera. The bovine papillomavirus (BPV) causes an infectious disease that is characterized by chronic and proliferative benign tumors that affect cattle worldwide. In the present work, the full genome sequence of BPV type 5, an Epsilonpapillomavirus, is reported. The genome was recovered from papillomatous lesions excised from cattle raised in the Amazon region, Northern Brazil. The genome comprises 7836 base pairs and exhibits the archetypal organization of the Papillomaviridae. This is of significance for the study of BPV biology, since currently available full BPV genome sequences are scarce. The availability of genomic information of BPVs can provide better understanding of the differences in genetics and biology of papillomaviruses.
Subject(s)
Cattle Diseases/virology , Genome, Viral , Papillomaviridae/classification , Papillomaviridae/genetics , Animals , Brazil , Cattle , DNA, Viral , Gene Order , Open Reading Frames , Papillomavirus Infections/veterinary , Phylogeny , Sequence Analysis, DNAABSTRACT
A co-infection comprising to at least seven papillomavirus (PV) types was detected by next generation sequencing (NGS) of randomly primed rolling circle amplification (RCA) products of a bovine (Bos taurus) papilloma lesion from the Brazilian Amazon region. Six putative new PV types that could not be detected by commonly used PCR protocols were identified. Their overall L1 nucleotide identities were less than 90% compared to described PV species and types. L1 nucleotide BLAST sequence hits showed that each new type was related to Beta, Gamma, Dyokappa, Dyoeta, and Xipapillomavirus, as well as two likely new unclassified genera. Our results show that the employment of NGS is relevant to the detection and characterization of distantly related PV and is of major importance in co-infection studies. This knowledge will help us understand the biology and pathogenesis of PV, as well as contribute to disease control. Moreover, we can also conclude that there are many unknown circulating PVs.
Subject(s)
Papilloma/virology , Papillomaviridae/genetics , Animals , Base Sequence , Cattle , Coinfection/veterinary , Coinfection/virology , DNA, Viral/chemistry , DNA, Viral/isolation & purification , DNA, Viral/metabolism , Genome, Viral , High-Throughput Nucleotide Sequencing , Nucleic Acid Amplification Techniques , Open Reading Frames/genetics , Papilloma/pathology , Papilloma/veterinary , Papillomaviridae/classification , Papillomaviridae/isolation & purification , Phylogeny , Polymerase Chain Reaction , Sequence Analysis, DNAABSTRACT
Currently, fifteen bovine papillomavirus (BPV) types have been identified and classified into four genera: Deltapapillomavirus, Epsilonpapillomavirus, Dyoxipapillomavirus, and Xipapillomavirus. Here, the complete genome sequence of a new BPV type (BPV 04AC14) recovered from a papillomatous lesion is reported. The genome is 7,282 bp in length and exhibits the classic genetic organization and motifs of the members of Papillomaviridae. Maximum likelihood phylogenetic analyses revealed that BPV 04AC14 clusters with members of the Xipapillomavirus genus. The nucleotide sequence of the L1 capsid protein of the novel BPV is closely related to its counterpart, BPV3, with which it shares 79% similarity. These findings suggest that this virus is a new BPV type of the Xipapillomavirus genus.
Subject(s)
High-Throughput Nucleotide Sequencing/methods , Papillomaviridae/genetics , Papillomaviridae/isolation & purification , Polymerase Chain Reaction/methods , Amino Acid Sequence , Animals , Base Sequence , Cattle , DNA, Intergenic/genetics , Epithelium/pathology , Epithelium/virology , Genome, Viral , Likelihood Functions , Phylogeny , Viral Proteins/chemistry , Viral Proteins/geneticsABSTRACT
Papillomaviruses are small and complex viruses that belong to the Papillomaviridae family, which comprises 39 genera. The bovine papillomavirus (BPV) causes an infectious disease that is characterized by chronic and proliferative benign tumors that affect cattle worldwide. Different genotypes of BPVs can cause distinct skin and mucosal lesions and the immunity they raise has low cross-protection. This report aimed to genotype BPVs in cattle from Northern Brazil based on nucleotide partial sequences of the L1 ORF. Skin wart samples from 39 bovines clinically and histopathologically diagnosed as cutaneous papillomatosis from Acre and Rondônia States were analyzed. The results revealed four already reported BPV types (BPVs 1, 2, 11, and 13), nine putative new BPV subtypes and four putative new BPV types as well as two putative new BPV types that were already reported. To our knowledge, this is the first record of BPVs from the Brazilian Amazon region that identified new possible BPV types and subtypes circulating in this population. These findings point to the great genetic diversity of BPVs that are present in this region and highlight the importance of this knowledge before further studies about vaccination are attempted.
