Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 4 de 4
Filter
Add more filters










Database
Language
Publication year range
1.
Nanoscale Res Lett ; 14(1): 166, 2019 May 17.
Article in English | MEDLINE | ID: mdl-31102112

ABSTRACT

For many cancers, early detection is the key to improve survival and reduce the morbidity, which is associated with radical resections due to late diagnosis. Here, we describe the efficiency of primary antibody-conjugated gold nanoparticles (AuNPs) to specifically target chronic inflammatory processes, specially M2 macrophages, in tissue sections of ulcerative colitis (UC) and steatohepatitis in rats which may lead to colorectal cancer and liver carcinoma, respectively. In this study, we demonstrate that AuNPs synthesized by a simple, inexpensive, and environmentally compatible method can be easily conjugated with the antibodies anti-COX-2, anti-MIF, and Alexa Fluor® 488 (ALEXA) to perform immunofluorescence staining in inflamed tissues. Moreover, we showed that primary antibody-conjugated gold nanoparticles (AuNPs) can be used to target M2 macrophages by flow cytometry. We designed three immunofluorescence staining protocols of tissue section with AuNPs for 30 min and overnight incubation, as well as one flow cytometry protocol of M2 macrophage labeling with AuNPs for 30 min. Immunofluorescence and flow cytometry results suggest that conjugation was achieved by direct adsorption of antibodies on the AuNPs surface. When compared to the standard ALEXA protocol in immunofluorescence (IF) and flow cytometry (FC), our 30-min incubation protocol using AuNPs instead of ALEXA decreased from approximately 23 h to 5 h for IF and from 4 h to 1 h for FC, proving to be less laborious, which makes the method eligible for inflammation-induced cancer diagnostic.

2.
Int J Pharm ; 548(1): 1-14, 2018 Sep 05.
Article in English | MEDLINE | ID: mdl-29886101

ABSTRACT

This study aimed to elucidate the anti-inflammatory, anti-oxidant and antifibrotic effects of gold nanoparticles (GNPs) in rats subjected to liver injury with ethanol and Methamphetamine (METH). The liver injury was induced by gavage administrations of 30% alcoholic solution (7 g/kg) once a day during 28 days, followed by METH (10 mg/kg) on the 20th and 28th days of treatment. GNPs treatment (724.96 µg/kg) during the ethanol and METH exposure was associated with reduced steatosis, hepatic cord degeneration, fibrosis and necrosis. Furthermore, there was a reduction in biochemical markers of liver damage and oxidative stress, and pro-inflammatory cytokines IL-1ß and TNF-α, compared to ethanol + METH group alone. A decrease of FGF, SOD-1 and GPx-1 expression was also observed. GNPs down-regulated the activity of Kupffer cells and hepatic stellate cells affecting the profile of their pro-inflammatory cytokines, oxidative stress and fibrosis through modulation of signaling pathways AKT/PI3K and MAPK in ethanol + METH-induced liver injury in a rat model.


Subject(s)
Anti-Inflammatory Agents/therapeutic use , Antioxidants/therapeutic use , Chemical and Drug Induced Liver Injury/drug therapy , Gold/therapeutic use , Liver Cirrhosis/drug therapy , Metal Nanoparticles/therapeutic use , Animals , Anti-Inflammatory Agents/pharmacokinetics , Antioxidants/pharmacokinetics , Chemical and Drug Induced Liver Injury/metabolism , Chemical and Drug Induced Liver Injury/pathology , Cytokines/metabolism , Ethanol , Fibroblast Growth Factors/metabolism , Glutathione/metabolism , Glutathione Peroxidase/metabolism , Gold/pharmacokinetics , Liver Cirrhosis/metabolism , Liver Cirrhosis/pathology , Male , Malondialdehyde/metabolism , Methamphetamine , Mice , NF-kappa B/genetics , Oxidative Stress/drug effects , Peroxidase/metabolism , RAW 264.7 Cells , Rats , Rats, Wistar , Superoxide Dismutase-1/metabolism , Glutathione Peroxidase GPX1
3.
Biophys Chem ; 238: 22-29, 2018 07.
Article in English | MEDLINE | ID: mdl-29723717

ABSTRACT

Understanding the interactions between nanoparticles and biological surfaces is of great importance for many areas of nanomedicine and calls for detailed studies at the molecular level using simplified models of cellular membranes. In this paper, water-dispersed polyvinylpyrrolidonestabilized gold nanoparticles (AuNPs) were incorporated in floating monolayers of selected lipids at the air-water interface as cell membrane models. Surface pressure-area isotherms showed the condensation of glycoside-free lipid monolayers, suggesting their adsorption on the nanoparticle surface through the hydrophilic head groups. On the other hand, monolayers containing glycoside derivatives expanded upon AuNPs incorporation, pointing that the supramolecular structure formed should facilitate the incorporation of these nanoparticles in cellular membranes. These findings can be therefore correlated with the possible toxicity, microbicide and antitumorigenic effects of these nanoparticles in lipidic surfaces of erythrocyte and microbial membranes.


Subject(s)
Air , Cell Membrane/chemistry , Gold/chemistry , Lipopolysaccharides/chemistry , Metal Nanoparticles/chemistry , Peptidoglycan/chemistry , Water/chemistry , Models, Chemical , Particle Size , Surface Properties
4.
J Colloid Interface Sci ; 512: 792-800, 2018 Feb 15.
Article in English | MEDLINE | ID: mdl-29112930

ABSTRACT

The antimicrobial property of silver nanoparticles (AgNPs) is believed to be associated to their interaction with biointerfaces such as microbial cell membranes, encouraging research on the identification of membrane sites capable of AgNPs binding. Although significant progress has been made in that regard, the exact molecular mechanism of action is yet to be fully understood. In this study, AgNPs dispersed in aqueous media and stabilized with polyvinylpyrrolidone were incorporated in Langmuir monolayers of selected lipids that served as cell membrane models. Results from pressure-area isotherms, vibrational spectroscopy and Brewster angle microscopy revealed condensation of glycoside-free lipid monolayers, evidencing that the AgNPs interact mostly with the lipid hydrophilic head groups. In contrast, the monolayers of systems containing glycoside derivatives were found to expand upon AgNPs incorporation, indicating that the glycosidic compounds might facilitate the incorporation of these nanoparticles in cellular membranes. These data can be therefore correlated with the possible toxicity and microbicide effect of AgNPs in lipidic surfaces of mammalian and microbial membranes.


Subject(s)
Air , Anti-Bacterial Agents/chemistry , Cell Membrane/chemistry , Metal Nanoparticles/chemistry , Povidone/chemistry , Silver/chemistry , Water/chemistry , Hydrophobic and Hydrophilic Interactions , Surface Properties
SELECTION OF CITATIONS
SEARCH DETAIL
...