ABSTRACT
Field bio-remediation techniques (FBRT) can be a low cost method to avoid the removal of top layers of soil which are rich in organic matter and bio diversity. The use of native microorganisms in FBRT is preferable because non-indigenous species can transfer their genetic material to the environment with negative impacts on the local ecological equilibrium. Petroleum Produced Water (PPW) is an important pollutant source in onshore production areas. However, due to high sodium concentrations in PPW and the occurrence of organic matter in dissolved and dispersed forms, obtaining pollutant transport parameters may be a difficult task. Results of column tests performed using a natural soil permeated by PPW are presented. All the samples presented a permeability decrease over time and the total hydrocarbon petroleum (TPH) breakthrough curves presented evidence of biological decay. Soil samples underwent biological characterization after tests (Metagenomic analyses and cultural media tests). Curves were modelled in an incremental way using a non-constant decay rate to better simulate the growing process of the microorganisms and consider the occurrence of varying velocity/permeability. Biological characterization results indicate the native organisms that are potentially more able to degrade PPW, including four bacteria (Bacillus and Lysinibacillus genus) and two fungi species (Malassezia and Talaromyces genus) that have not previously been mentioned in the consulted literature. The obtained results contribute to the development of more sustainable FBRTs focusing on native microorganisms, already adapted to the local environmental conditions.