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Carbohydr Polym ; 91(1): 467-75, 2013 Jan 02.
Article in English | MEDLINE | ID: mdl-23044157

ABSTRACT

Fucan (SV1) sulfated polysaccharides from the brown algae Sargassum vulgare were extracted, fractionated in acetone and examined with respect to chemical composition, anticoagulant, anti-inflammatory, antithrombotic effects and cellular proliferation. These polysaccharides contain low levels of protein, high level of carbohydrate and sulfate. Monosaccharides analysis revealed that SV1 was composed of fucose, galactose, xylose, glucuronic acid and mannose. SV1 polysaccharide prolonged activated partial thromboplastin time (aPTT) and exhibited high antithrombotic action in vivo, with a concentration ten times higher than heparin activity. PSV1, a purified form in gel filtration showed very low biological activities. SV1 stimulated the enzymatic activity of FXa. Its action on DPPH radical scavenging activity was 22%. This polymer has no cytotoxic action (hemolytic) on ABO and Rh blood types in different erythrocyte groups. It displays strong anti-inflammatory action at all concentrations tested in the carrageenan-induced paw edema model, demonstrated by reduced edema and cellular infiltration.


Subject(s)
Polysaccharides/pharmacology , Sargassum/chemistry , ABO Blood-Group System/metabolism , Animals , Anti-Inflammatory Agents, Non-Steroidal/chemistry , Anti-Inflammatory Agents, Non-Steroidal/isolation & purification , Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Anti-Inflammatory Agents, Non-Steroidal/therapeutic use , Anticoagulants/chemistry , Anticoagulants/isolation & purification , Anticoagulants/pharmacology , Anticoagulants/therapeutic use , Carrageenan/adverse effects , Cell Line, Tumor , Cell Survival/drug effects , Edema/chemically induced , Edema/drug therapy , Erythrocytes/drug effects , Erythrocytes/immunology , Factor Xa/metabolism , Fibrinolytic Agents/chemistry , Fibrinolytic Agents/isolation & purification , Fibrinolytic Agents/pharmacology , Fibrinolytic Agents/therapeutic use , Free Radical Scavengers/chemistry , Free Radical Scavengers/isolation & purification , Free Radical Scavengers/pharmacology , Free Radical Scavengers/therapeutic use , Hemolysis/drug effects , Male , Mice , Polysaccharides/chemistry , Polysaccharides/isolation & purification , Polysaccharides/therapeutic use , Rats , Rats, Wistar , Rh-Hr Blood-Group System/metabolism , Thrombin/metabolism
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