Effects of photobiomodulation with blue Light Emitting Diode (LED) on the healing of skin burns.

The management of skin burns is still challenging. Among the therapeutic methods used, there are topical treatments with pharmacological and herbal agents, low-intensity therapeutic ultrasound, use of biomaterials, reconstructive techniques and photobiomodulation therapy. The aim of this study was to evaluate the effects of photobiomodulation with blue Light Emitting Diode (LED) on burn healing. Fifty Wistar rats were divided into control (CTRL) (n = 25) and blue LED (LED) (n = 25), with subgroups (n = 5) for each time of euthanasia (7, 14, 21, 28 and 32 days). Treated animals were daily irradiated (470 nm, 1W, 0.44 W/cm2, 50 J/cm2). Clinical evaluations were performed and the Wound Retraction Index (WRI) was determined. Histological sections were submitted to hematoxylin-eosin, toluidine blue and the immunohistochemical technique, with anti-α-SMA and anti-TGF-ß1 antibodies. All data were directly collected by previously calibrated evaluators in a blind manner. The values were included in a statistical program. For all statistical tests used, 5% significance level (p < 0.05) was considered. No statistically significant differences in WRI between groups were observed (p > 0.05). Re-epithelialization was higher using LED at 7 and 14 days (p < 0.05) and greater amount of inflammatory cells was observed at 7 days (p = 0.01). With LED at 21 and 32 days, greater number of mast cells were observed (p < 0.05), as well as smaller number of myofibroblasts at 14, 21, 28 and 32 days (p < 0.05) and lower percentage of TGF-ß1 positive cells in the conjunctiva at 7, 14 and 21 days (p < 0.05). Negative correlations were observed in LED between the percentage of TGF-ß1 in the epithelium and the mean number of inflammatory cells and number of myofibroblasts (p < 0.05). The results suggest that, depending on the period, blue LED can modulate the healing processes of third-degree skin burns, such as re-epithelialization, inflammatory response, mast cell concentration, myofibroblast differentiation and TGF-ß1 immunoexpression. Despite these effects, this therapy does not seem to have significant influence on the retraction of these wounds. Future studies, using different protocols, should be carried out to expand the knowledge about the photobiomodulatory mechanisms of this type of light in the healing process.

Effects of photobiomodulation therapy with red LED on inflammatory cells during the healing of skin burns.

The aim of this study was to evaluate the effects of red light emitting diode (LED) photobiomodulation therapy protocol on inflammatory cells during the healing of third-degree skin burns. Fifty Wistar rats were randomly divided into control group (CTRL) (n = 25) and red group (RED) (n = 25), with subgroups (n = 5) for each time of euthanasia (7, 14, 21, 28, and 32 days). Treatment animals were daily irradiated (630 nm ± 10 nm, 300 mW, 9 J/cm2 per point, 30 s, continuous emission mode) at the 4 angles of the wound (total: 36 J/cm2). After specimen removal, histological sections were stained with hematoxylin and eosin for quantitative analysis of the inflammatory infiltrate (neutrophils and lymphocytes) under light microscopy. Greater number of inflammatory cells was observed in irradiated groups when compared to CTRL at 7, 14, 21, and 28 days, but with statistically significant difference only at 14 days (p = 0.02). At 32 days, higher inflammatory cell value was observed in CTRL when compared to RED, but with no statistically significant difference (p = 0.91). The results suggest that red LED, according to the protocol used, modulates the number of inflammatory cells in the early stages of the healing of third-degree skin burns. Nevertheless, this low-intensity light therapy may not, be efficient in reducing the number of neutrophils and lymphocytes in advanced stages of the repair process of skin burns. Further studies with other therapy protocols are needed to assess the effects of this type of light on the inflammatory response of skin burns.

Effect of blue LED on the healing process of third-degree skin burns: clinical and histological evaluation.

The aim of this study was to evaluate the effects of blue light-emitting diode (LED) on the healing process of third-degree skin burns in rats through clinical and histological parameters. Forty male Wistar rats were divided into two groups: control (CTR) (n = 20) and blue LED (BLUE) (n = 20), with subgroups (n = 5) for each time of euthanasia (7, 14, 21, and 28 days). LED (470 nm, 1 W, 12.5 J/cm2 per point, 28 s) was applied at four points of the wound (total, 50 J/cm2). Feed intake was measured every other day. It was observed that there were no statistically significant differences in the Wound Retention Index (WRI) of the BLUE group in relation to CTR group (p > 0.05) at the evaluation times. After 14, 21, and 28 days, it was observed that the animals in the BLUE group consumed more feed than animals in the CTR group (p < 0.05). At 7 days, there was a statistically significant increase in the angiogenic index (AI) in BLUE (median: 6.2) when compared to CTR (median: 2.4) (p = 0.01) and all animals in BLUE had already started re-epithelialization. This study suggests that blue LED, at the dosimetry used, positively contributed in important and initial stages of the healing process of third-degree skin burns.