ABSTRACT
Tomato chlorotic spot virus (TCSV) and groundnut ringspot virus (GRSV) share several genetic and biological traits. Both of them belong to the genus Tospovirus (family Peribunyaviridae), which is composed by viruses with tripartite RNA genome that infect plants and are transmitted by thrips (order Thysanoptera). Previous studies have suggested several reassortment events between these two viruses, and some speculated that they may share one of their genomic segments. To better understand the intimate evolutionary history of these two viruses, we sequenced the genomes of the first TCSV and GRSV isolates ever reported. Our analyses show that TCSV and GRSV isolates indeed share one of their genomic segments, suggesting that one of those viruses may have emerged upon a reassortment event. Based on a series of phylogenetic and nucleotide diversity analyses, we conclude that the parental genotype of the M segment of TCSV was either eliminated due to a reassortment with GRSV or it still remains to be identified.
Subject(s)
Genome, Viral , Reassortant Viruses , Solanum lycopersicum/virology , Tospovirus/genetics , Animals , Evolution, Molecular , Genetic Variation , High-Throughput Nucleotide Sequencing , Phylogeny , RNA, Viral/genetics , Thysanoptera/virologyABSTRACT
The Tomato chlorotic spot virus (TCSV) was first reported in the 1980s, having its occurrence limited to Brazil and Argentina. Due to an apparent mild severity in the past, molecular studies concerning TCSV were neglected. However, TCSV has disseminated over the USA and Caribbean countries. In Dominican Republic TCSV has been recently reported on important cultivated crops such as pepper and beans. In this work, we provide the first complete genome of a TCSV isolate from symptomatic plants in Dominican Republic, which was obtained by high-throughput sequencing. In addition, three dsRNA viruses from different virus families were identified coinfecting these plants Bell pepper endornavirus (BPEV), Southern tomato virus (STV) and Pepper cryptic virus 2 (PCV-2). Phylogenetic analysis showed that the Dominican Republic TCSV isolate has a close relationship with other TCSV isolates and a reassortant isolate between TCSV and Groundnut ringspot virus (GRSV), all found in USA. BPEV, STV and PCV-2 isolates from Dominican Republic were close related to corresponding American isolates. The possible biological implications of these virus-mixed infections are discussed.
Subject(s)
Coinfection , Genome, Viral , Plant Diseases/virology , RNA Viruses/classification , RNA Viruses/genetics , Tospovirus/classification , Tospovirus/genetics , Vegetables/virology , Dominican Republic , High-Throughput Nucleotide Sequencing , Phenotype , Phylogeny , RNA Viruses/isolation & purification , RNA, Double-Stranded , RNA, Viral , Tospovirus/isolation & purificationABSTRACT
Begomoviruses are plant viruses responsible for severe losses in important crops, such as beans, cassavas, tomatoes, and cotton, around the world. Here, we report the first full-genome sequence of a bipartite begomovirus species collected from cotton plants in Brazil.
