Subject(s)
Bacterial Infections/diagnosis , Gram-Negative Anaerobic Bacteria/isolation & purification , Abscess/microbiology , Animals , Anti-Bacterial Agents/pharmacology , Bacteria/isolation & purification , Bacteriological Techniques , Drug Resistance, Microbial , Leg Ulcer/microbiology , Osteomyelitis/microbiologyABSTRACT
Samples collected from the dental surface, gingival sulcus, dorsum of the tongue and from the cheek of ten adults (18 to 32 years) were investigated to identify the Veillonella and Neisseria species present by applying the taxonomic criteria suggested in the latest edition of Bergey's Manual. The various niches contain both V. parvula and V. alcalescens, but with the predominance of strains which do not fit precisely the described catalase and putrescine requirements. Eighty-nine out of 100 isolated strains of Gram negative oxidase positive cocci could be identified as N. sicca and N. subflava; about 11 unidentified strains exhibited characteristics of N. flavescens species.
Subject(s)
Mouth/microbiology , Neisseria/isolation & purification , Veillonella/isolation & purification , Adolescent , Adult , Cheek/microbiology , Gingiva/microbiology , Humans , Neisseria/classification , Tongue/microbiology , Tooth/microbiology , Veillonella/classificationABSTRACT
Strains of Neisseria sicca and N. mucosa, but not N. perflava, N. subflava, N. flava, or N. flavescens were found to grow on eosin methylene blue agar. The distribution of N. sicca on the tongue dorsum, the gingival crevice area, and the coronal surfaces of teeth of humans was determined using this medium. N. sicca averaged about 5% of the total cultivable organisms of the tongues of 14 subjects examined, but it was present in only trace quantities in dental plaque on the coronal surfaces of teeth or in the gingival crevice area.
