Effect of incorporating modified pinhão starch in alginate-based hydrogel beads for encapsulation of bioactive compounds by hydrodynamic electrospray ionization jetting.

Known for its antioxidant properties, Araucaria angustifolia bracts extract was encapsulated using hydrodynamic electrospray ionization jetting within calcium alginate cross-linked hydrogel beads with varying contents of modified pinhão starch. The rheological properties of the dispersions and analysis of the physicochemical and digestive properties of encapsulated beads were studied. The results demonstrated that dispersions containing starch exhibited higher viscosity and reduced compliance values, indicating samples with stronger, more compact, and stable structures that are less susceptible to deformation. This was confirmed by the beads rupture strength test. The ATR-FTIR analysis suggest that no new chemical bonds were formed, with encapsulation being responsible only for physical interactions between the functional groups of the polymers used and the active groups of the compounds present in the extract. The thermal stability of starch-containing beads was higher. Total tannins were higher in beads containing starch, with 53.61 %, 56.83 %, and 66.99 % encapsulation yield for samples with 2 %, 4 %, and 6 % starch, respectively, and the remaining antioxidant activity ranged from 96.04 % to 81.08 %. In vitro gastrointestinal digestion simulation indicated that the highest releases occurred in the intestinal phase, ranging from 60.72 % to 63.50 % for the release of total phenolic compounds.

Effect of microwave followed by cooling on structural and digestive properties of pinhão starch.

To increase its resistant content, native pinhão starch was modified using a microwave (300 W, 90 s) and subsequently cooled at 4 °C for 4, 8, 16, 24, and 72 h. The results demonstrated that all starches exhibited a crystalline structure of type C, with decreased crystallinity after modification. In the modified samples, the ratio of peaks 1047/1022 cm-1 and 995/1022 cm-1, as identified by FTIR, indicated a reduction in the crystalline region and damage to the double helix structure of starch granules. DSC analysis revealed that modified starches had lower gelatinization temperature range values due to the presence of more homogeneous crystals. Rheological analyses showed that starch suspensions obtained exhibited pseudoplastic fluid behavior and gel-like viscoelastic structure formation, with higher storage moduli in samples with longer cooling times. The microwave-modified starch, cooled for 72 h, exhibited higher digestion resistance, resulting in a 43.6 % increase in resistant starch content and a 26.1 % decrease in rapidly digestible starch compared to native starch. The results highlight that the modification of native pinhão starch using a microwave, followed by cooling at 4 °C for 72 h, presents a promising method for increasing the resistant starch content.