The effect of laser therapy on the proliferation of oral KB carcinoma cells: an in vitro study.

OBJECTIVE: The aim of this study was to assess the proliferative effect of carcinoma cells, strain KB, submitted to laser therapy with wavelengths of lambda685nm (31 mW; Ø; 0.38 cm(2), 4 J/cm(2)) or lambda830nm (34.5 mW; Ø; 0.38 cm(2), 4 J/cm(2)). BACKGROUND DATA: It is known that the interaction of laser light with living tissues may lead to different results depending upon several factors such as wavelength, dose, potency, and optical properties of the tissue as well as on the condition being treated. The response to the use of laser light may be of stimulation or inhibition. One successful model used to study the effects of laser light on living tissues is the in vitro use of different lineages of cells in culture. METHODS: Cellular viability was assessed using MTT spectroscopy immediately, and 6, 12, 24, and 72 h after treatment. The irradiations were carried out twice, at 24 h after cell seeding and at 48 h after the first irradiation. The dose of 4 J/cm(2) was given by a lambda685 nm (31 mW, Phi 0.8 cm(2)) or lambda830 nm (34.5 mW, Phi 0.8 cm(2)) diode lasers. RESULTS: The results demonstrated that the time influenced significantly both control (p = 0.01) and both cultures irradiated with lambda685-nm laser (p = 0.01) or lambda830-nm laser (p = 0.09). The influence of the treatment (laser therapy) was also significant when comparing the results observed in irradiated groups and the control (p = 0.01). The influence of the wavelength in the final result, in other words, in the cellular viability of cultures irradiated with the two wavelengths was also significant (p = 0.01). CONCLUSIONS: It is concluded that laser therapy had a positive biomodulatory effect on the proliferation of KB cells and that this was influenced by the wavelength.

Laser light is capable of inducing proliferation of carcinoma cells in culture: a spectroscopic in vitro study.

OBJECTIVE: The objective of this work was to evaluate the influence of time, treatment, and wavelength, through the assessment of the cellular viability with MTT, on the proliferation of H.Ep.2 cells subjected to laser irradiation or not (lambda685 and lambda830 nm) with the same energy density (4 J/cm2). BACKGROUND DATA: Although malignant lesions have been studied for some time, there is not yet a definitive cure. Mortality could be reduced if lesions were diagnosed on initial phases of development. MATERIALS AND METHODS: H.Ep.2 cells were cultured in flasks and maintained in DMEN medium (10% FBS, 1% L-glutamine, and 1% antibiotic solution). For irradiation, cells were kept in 24 wells of the 96-well plaques containing DMEM medium (5% FBS, 1% L-glutamine, and 1% antibiotic solution), irradiated with lasers at lambda685- and lambda830-nm wavelength, and stained at 0, 6, 12, 24, and 48 h after irradiation. RESULTS: There was significant differences when the three groups were compared (p = 0.0087). There was significant difference for both irradiated groups, lambda685 nm (p = 0.0202) and lambda830 nm (p = 0.0324). Time of irradiation significantly influenced only the lambda685-nm group (p = 0.04). The wavelength had a significant influence (p = 0.013). CONCLUSION: Time, treatment, and wavelength significantly influenced the proliferation process of H.Ep.2 cells.