ABSTRACT
A new commercial low-density microarray which identifies common extended-spectrum ß-lactamase plasmid-mediated cephalosporinase genes, as well as carbapenemase (bla(KPC) and bla(NDM)) genes, was evaluated. We tested 207 clinical and reference/collection isolates of the Enterobacteriaceae possessing different bla genes. Overall, the sensitivity and specificity of the microarray were 100% for the detection of the plasmid-mediated bla(AmpC), bla(KPC), and bla(NDM) genes using bla gene sequencing as the reference method.
Subject(s)
Gram-Negative Bacteria/drug effects , Gram-Negative Bacteria/enzymology , Oligonucleotide Array Sequence Analysis/methods , beta-Lactams/pharmacology , Gram-Negative Bacteria/genetics , Plasmids/genetics , beta-Lactamases/geneticsABSTRACT
Five multidrug-resistant nonclonally related Enterobacteriaceae isolates were recovered in Belgium in 2010 from three patients who had been hospitalized in Pakistan, Montenegro, and Serbia/Kosovo. New Delhi metallo-ß-lactamase (NDM-1) was detected in each of the isolates in addition to several extended-spectrum ß-lactamases (CTX-M-15, SHV-12), plasmidic cephalosporinases (CMY-16, CMY-58), rRNA methylases (ArmA, RmtB), and Qnr genes (qnrA6, qnrB1, qnrB2). One patient died from uncontrolled sepsis, while the two others recovered. No secondary cases occurred in any of the hospitals.
