Subject(s)
Cat Diseases/microbiology , Empyema, Pleural/veterinary , Streptococcal Infections/veterinary , Animals , Cat Diseases/genetics , Cat Diseases/pathology , Cats , Diagnosis, Differential , Empyema, Pleural/etiology , Empyema, Pleural/microbiology , Female , Male , Pleura/microbiology , Pleura/pathology , RNA, Ribosomal/analysis , Restriction Mapping , Streptococcal Infections/genetics , Streptococcal Infections/pathologySubject(s)
Iguanas/microbiology , Salmonella Infections, Animal/transmission , Salmonella Infections/transmission , Salmonella/classification , Zoonoses , Animals , Animals, Domestic , DNA, Ribosomal/genetics , Female , Genes, Bacterial , Humans , Infant, Newborn , Male , Polymorphism, Restriction Fragment Length , Restriction Mapping , Salmonella/genetics , Salmonella/isolation & purification , Salmonella Infections/microbiologyABSTRACT
A genetic map of Ovis aries (haploid n = 27) was developed with 519 markers (504 microsatellites) spanning approximately 3063 cM in 26 autosomal linkage groups and 127 cM (female specific) of the X Chromosome (Chr). Genotypic data were merged from the IMF flock (Crawford et al., Genetics 140, 703, 1995) and the USDA mapping flock. Seventy-three percent (370/504) of the microsatellite markers on the map are common to the USDA-ARS MARC cattle linkage map, with 27 of the common markers derived from sheep. The number of common markers per homologous linkage group ranges from 5 to 22 and spans a total of 2866 cM (sex average) in sheep and 2817 cM in cattle. Marker order within a linkage group was consistent between the two species with limited exceptions. The reported translocation between the telomeric end of bovine Chr 9 (BTA 9) and BTA 14 to form ovine Chr 9 is represented by a 15-cM region containing 5 common markers. The significant genomic conservation of marker order will allow use of linkage maps in both species to facilitate the search for quantitative trait loci (QTLs) in cattle and sheep.
Subject(s)
Chromosome Mapping , Genetic Linkage , Sheep/genetics , Animals , Conserved Sequence , Female , Microsatellite RepeatsABSTRACT
We report the evaluation of 1036 bovine microsatellite primer pairs for their suitability as linkage markers in sheep. Approximately 58% (605/1036) of bovine primer pairs amplified a locus in sheep. Sixty-seven per cent (409/605) of amplified loci were detected as polymorphic. Marker heterozygosity, allele number and range of allele sizes were significantly lower in sheep than cattle sampled in this study. However, median fragment size was similar. These data suggest that high-resolution comparative linkage maps between closely related species can be constructed relatively efficiently.
Subject(s)
Cattle/genetics , Microsatellite Repeats , Sheep/genetics , Alleles , Animals , Conserved Sequence , DNA Primers , Evolution, Molecular , Genome , Heterozygote , Polymerase Chain Reaction , Species SpecificityABSTRACT
The red flour bettle, Tribolium castaneum, is both a pest of stored grain products and an important experimental organism. To improve its facility as a genetic model, we are developing DNA fingerprinting methods for this insect. A Tribolium DNA fragment, snapback-1 (SBI), identified among sequences that reassociate before a Cot of 0.03 mol.s/L, was found to produce a banding pattern in restriction endonuclease digested genomic DNA that is characteristic of a midrepetitive element. DNA fingerprints of individual beetles demonstrated that unvarying inherited DNA polymorphism is revealed, and that polymorphism is inherited in a dominant Mendelian fashion. Linkage between bands was minimal. The sequence of SBI was determined, and hybridization experiments indicated that SBI is a fragment of a larger midrepetitive element. Fingerprinting individuals with known inbreeding coefficients indicated that SBI loci have relatively high mutation rates. The possibility that SBI is a fragment of a transposable element is discussed.
