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1.
Infect Immun ; 69(10): 6217-24, 2001 Oct.
Article in English | MEDLINE | ID: mdl-11553563

ABSTRACT

Enteropathogenic Escherichia coli (EPEC) is an extracellular bacterial pathogen that infects the human intestinal epithelium and is a major cause of infantile diarrhea in developing countries. EPEC belongs to the group of attaching and effacing (A/E) pathogens. It uses a type III secretion system to deliver proteins into the host cell that mediate signal transduction events in host cells. We used gene array technology to study epithelial cell responses to EPEC infection at the level of gene expression. We found that EPEC induces the expression of several genes in infected HeLa cells by a lipopolysaccharide (LPS)-independent mechanism, including cytokines and early growth response factor 1 (Egr-1). The transcription factor Egr-1 is an immediate-early-induced gene that is activated in most cell types in response to stress. EPEC-induced upregulation of egr-1 is mediated by the activation of the MEK/extracellular signal-regulated kinase signal transduction pathway and is dependent on the type III secretion system. egr-1 is also induced during infection of mice by the A/E pathogen Citrobacter rodentium, suggesting that both Egr-1 and the activation of this mitogen-activated protein kinase signal transduction pathway may play a role in disease.


Subject(s)
DNA-Binding Proteins/genetics , Escherichia coli Infections/metabolism , Immediate-Early Proteins/genetics , MAP Kinase Signaling System , Transcription Factors/genetics , Animals , Blotting, Northern/methods , Citrobacter freundii , Early Growth Response Protein 1 , Enterobacteriaceae Infections/metabolism , Enzyme Activation , Epithelial Cells , Gene Expression , HeLa Cells , Humans , Interleukin-8/genetics , Lipopolysaccharides/pharmacology , Mice , Mice, Inbred C57BL , Mitogen-Activated Protein Kinase 1/metabolism , Mitogen-Activated Protein Kinase 3 , Mitogen-Activated Protein Kinases/metabolism , Phosphorylation , Reverse Transcriptase Polymerase Chain Reaction
2.
Infect Immun ; 68(7): 4344-8, 2000 Jul.
Article in English | MEDLINE | ID: mdl-10858257

ABSTRACT

Enteropathogenic Escherichia coli (EPEC) inserts its receptor for intimate adherence (Tir) into host cell membranes by using a type III secretion system. Detergents are frequently used to fractionate infected host cells to investigate bacterial protein delivery into mammalian cells. In this study, we found that the Triton X-100-soluble membrane fraction from EPEC-infected HeLa cells was contaminated with bacterial proteins. We therefore applied a mechanical method of cell lysis and ultracentrifugation to fractionate infected HeLa cells to investigate the biology and biochemistry of Tir delivery and translocation. This method demonstrates that the translocation of Tir into the host cell membrane requires its transmembrane domains, but not tyrosine phosphorylation or binding to Tir's ligand, intimin.


Subject(s)
Bacterial Proteins/metabolism , Escherichia coli Proteins , Escherichia coli/metabolism , Escherichia coli/pathogenicity , Receptors, Cell Surface/metabolism , Bacterial Proteins/genetics , Bacterial Proteins/isolation & purification , Biological Transport, Active , Cell Fractionation/methods , Cell Membrane/metabolism , Cell Membrane/microbiology , Detergents , Escherichia coli/genetics , Genes, Bacterial , HeLa Cells , Humans , Mutation , Octoxynol , Peptide Fragments/genetics , Peptide Fragments/isolation & purification , Peptide Fragments/metabolism , Protein Structure, Tertiary , Receptors, Cell Surface/genetics , Receptors, Cell Surface/isolation & purification
3.
Plasmid ; 42(3): 241-5, 1999 Nov.
Article in English | MEDLINE | ID: mdl-10545266

ABSTRACT

The cloning vector pMK18 was developed through the fusion of the minimal replicative region from an indigenous plasmid of Thermus sp. ATCC27737, a gene cassette encoding a thermostable resistance to kanamycin, and the replicative origin and multiple cloning site of pUC18. Plasmid pMK18 showed transformation efficiencies from 10(8) to 10(9) per microgram of plasmid in Thermus thermophilus HB8 and HB27, both by natural competence and by electroporation. We also show that T. thermophilus HB27 can take pMK18 modified by the Escherichia coli methylation system with the same efficiency as its own DNA. To demonstrate its usefulness as a cloning vector, a gene encoding the beta-subunit of a thermostable nitrate reductase was directly cloned in T. thermophilus HB27 from a gene library. Its further transfer to E. coli also proved its utility as a shuttle vector.


Subject(s)
Thermus thermophilus/genetics , Transformation, Genetic , Cloning, Molecular , Genetic Vectors , Models, Genetic
4.
Mol Microbiol ; 33(6): 1162-75, 1999 Sep.
Article in English | MEDLINE | ID: mdl-10510231

ABSTRACT

Enteropathogenic Escherichia coli (EPEC) secretes several Esps (E. coli-secreted proteins) that are required for full virulence. Insertion of the bacterial protein Tir into the host epithelial cell membrane is facilitated by a type III secretion apparatus, and at least EspA and EspB are required for Tir translocation. An EPEC outer membrane protein, intimin, interacts with Tir on the host membrane to establish intimate attachment and formation of a pedestal-like structure. In this study, we identified a Tir chaperone, CesT, whose gene is located between tir and eae (which encodes intimin). A mutation in cesT abolished Tir secretion into culture supernatants and significantly decreased the amount of Tir in the bacterial cytoplasm. In contrast, this mutation did not affect the secretion of the Esp proteins. The level of tir mRNA was not affected by the cesT mutation, indicating that CesT acts at the post-transcriptional level. The cesT mutant could not induce host cytoskeletal rearrangements, and displayed the same phenotype as the tir mutant. Gel overlay and GST pulldown assays demonstrated that CesT specifically interacts with Tir, but not with other Esp proteins. Furthermore, by using a series of Tir deletion derivatives, we determined that the CesT binding domain is located within the first 100 amino-terminal residues of Tir, and that the pool of Tir in the bacterial cytoplasm was greatly reduced when this domain was disrupted. Interestingly, this domain was not sufficient for Tir secretion, and at least the first 200 residues of Tir were required for efficient secretion. Gel filtration studies showed that Tir-CesT forms a large multimeric complex. Collectively, these results indicate that CesT is a Tir chaperone that may act as an anti-degradation factor by specifically binding to its amino-terminus, forming a multimeric stabilized complex.


Subject(s)
Bacterial Proteins/physiology , Escherichia coli Proteins , Escherichia coli/physiology , Escherichia coli/pathogenicity , Molecular Chaperones/physiology , Receptors, Cell Surface/physiology , Amino Acid Sequence , Bacterial Proteins/genetics , Base Sequence , Binding Sites/genetics , DNA Primers/genetics , DNA, Bacterial/genetics , Escherichia coli/genetics , Genes, Bacterial , HeLa Cells , Humans , Macromolecular Substances , Molecular Chaperones/genetics , Molecular Sequence Data , Mutation , RNA, Bacterial/genetics , RNA, Bacterial/metabolism , RNA, Messenger/genetics , RNA, Messenger/metabolism , Receptors, Cell Surface/genetics , Sequence Deletion , Transcription, Genetic , Virulence/genetics , Virulence/physiology
5.
Trends Cell Biol ; 9(1): 11-4, 1999 Jan.
Article in English | MEDLINE | ID: mdl-10087611

ABSTRACT

Enteropathogenic Escherichia coli (EPEC) subverts host signalling pathways and the cytoskeleton during infection, resulting in disease characterized by diarrhoea. Recent studies have revolutionized our understanding of the infection process by showing that this bacterium inserts its own receptor into the plasma membrane overlying the host actin cytoskeleton. The reorganized actin forms a pedestal-like structure with the bacterium at the tip. This review discusses the mechanism of infection and pedestal formation and how this system might be a powerful tool for studying actin dynamics at the plasma membrane.


Subject(s)
Actins/metabolism , Escherichia coli/physiology , Signal Transduction , Animals , Bacterial Adhesion , Cytoskeleton , Diarrhea/physiopathology , Escherichia coli/pathogenicity , Virulence
6.
Cell Microbiol ; 1(1): 7-17, 1999 Jul.
Article in English | MEDLINE | ID: mdl-11207537

ABSTRACT

Enteropathogenic Escherichia coli (EPEC) attaches intimately to mammalian cells via a bacterial outer membrane adhesion molecule, intimin, and its receptor in the host cell membrane, Tir. Tir is a bacterial protein translocated into the host cell membrane and tyrosine phosphorylated after insertion. Tir-intimin binding induces organized actin polymerization beneath the adherent bacteria, resulting in the formation of pedestal-like structures. A series of Tir deletion derivatives were constructed to analyse which Tir domains are involved in intimin binding. We have localized the intimin-binding domain (IBD) of Tir using a yeast two-hybrid system and a gel-overlay approach to a region of 109 amino acids that is predicted to be exposed on the surface of the plasma membrane. A truncated Tir protein lacking this domain was translocated to the host cell membrane and tyrosine phosphorylated, but failed to bind intimin or to induce either actin polymerization or Tir accumulation beneath the bacteria. These results indicate that only a small region of Tir is needed to bind intimin and support the predicted topology for Tir, with both N- and C-terminal regions in the mammalian cell cytosol. They also confirm that Tir-intimin interactions are needed for cytoskeletal organization. We have also identified N-terminal regions involved in Tir stability and Tir secretion to the media.


Subject(s)
Adhesins, Bacterial , Bacterial Outer Membrane Proteins/metabolism , Carrier Proteins , Epithelial Cells/microbiology , Escherichia coli Proteins , Escherichia coli/pathogenicity , Receptors, Cell Surface/metabolism , Actins/metabolism , Bacterial Outer Membrane Proteins/chemistry , Bacterial Outer Membrane Proteins/genetics , Electrophoresis, Polyacrylamide Gel , Epithelial Cells/pathology , Escherichia coli/chemistry , Fluorescent Antibody Technique , Gene Deletion , HeLa Cells , Humans , Immunoblotting , Mutation , Phosphorylation , Protein Binding , Protein Structure, Tertiary/genetics , Receptors, Cell Surface/chemistry , Receptors, Cell Surface/genetics , Recombinant Proteins , Saccharomyces cerevisiae/genetics , Transfection , Tyrosine/metabolism
7.
FEMS Microbiol Lett ; 165(1): 51-7, 1998 Aug 01.
Article in English | MEDLINE | ID: mdl-9711839

ABSTRACT

The minimal replicative origin from a 16-kbp natural plasmid of Thermus sp. ATCC 27737 is described. The 1798-bp sequence encodes a single protein (RepA) whose expression is required for replication. This requirement is extended to 200 bp upstream the coding region, which contains promoter-like sequences transcriptionally active in E. coli. Despite the absence of typical iterons, we show that an N-terminal fragment of this protein binds to a DNA fragment internal to its own coding sequence. As this DNA fragment contains an unusually A + T rich sequence surrounded by C + G rich stretches, the possible location of the replicative origin within the region encoding RepA is proposed.


Subject(s)
Bacterial Proteins/genetics , Plasmids/genetics , Replication Origin/genetics , Thermus/genetics , Amino Acid Sequence , Bacterial Proteins/chemistry , Base Sequence , Cloning, Molecular , Molecular Sequence Data , Recombinant Fusion Proteins/analysis , Restriction Mapping , Thermus/growth & development , Transformation, Bacterial
8.
J Bacteriol ; 174(20): 6424-31, 1992 Oct.
Article in English | MEDLINE | ID: mdl-1400194

ABSTRACT

We describe the self-selection of replication origins of undescribed cryptic plasmids from Thermus aquaticus Y-VII-51B (ATCC 25105) and a Thermus sp. strain (ATCC 27737) by random insertion of a thermostable kanamycin adenyltransferase cartridge. Once selected, these autonomous replication origins were cloned into the Escherichia coli vector pUC9 or pUC19. The bifunctional plasmids were analyzed for their sizes, relationships, and properties as shuttle vectors for Thermus-Escherichia cloning. Seven different vectors with diverse kanamycin resistance levels, stabilities, transformation efficiencies, and copy numbers were obtained. As a general rule, those from T. aquaticus (pLU1 to pLU4) were more stable than those from the Thermus sp. (pMY1 to pMY3). To probe their usefulness, we used one of the plasmids (pMY1) to clone in E. coli a modified form of the cellulase gene (celA) from Clostridium thermocellum in which the native signal peptide was replaced in vitro by that from the S-layer gene of T. thermophilus HB8. The hybrid product was expressed and exported by E. coli. When the gene was transferred by transformation into T. thermophilus, the cellulase protein was also expressed and secreted at 70 degrees C.


Subject(s)
Cellulase/genetics , Clostridium/enzymology , Escherichia coli/genetics , Genetic Vectors/genetics , Thermus thermophilus/genetics , Amino Acid Sequence , Base Sequence , Cellulase/chemistry , Cellulase/metabolism , Cloning, Molecular , Clostridium/genetics , Genes, Bacterial/genetics , Plasmids/genetics , Restriction Mapping
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