Antimicrobial Potential of Chitosan Films Incorporated with Alcoholic Extract from Mimosa tenuiflora Leaves.

Antimicrobial films were prepared with chitosan containing the methanolic extract of M. tenuiflora leaves (FECT20%, FECT30%, and FECT40%), and their antimicrobial activities were evaluated by agar diffusion. The films were characterized by IR spectroscopy, scanning electron microscopy (SEM) and TG/DTG curves. TG/DTG curves showed thermal stability of chitosan-extract films up to 166 ºC. Micrographs of chitosan-extract films revealed an increase in porosity with the addition of extract. The FECT40% film showed inhibition zone diameters (IZ) against Micrococcus luteus, Staphylococcus aureus, Bacillus subtilis, and B. cereus, ranging from 1.0 ± 0.02 to 0.72 ± 0.09 cm. Only FECT30% and FECT40% inhibited the P. aeruginosa with IZs of 0.68 ± 0.02 and 0.77 ± 0.06 cm, respectively. In turn, the extract showed inhibition against B. subtilis and B. cereus, with IZs values of 0.92 ± 0.2 cm and 0.72 ± 0.05 cm, respectively. Additionally, the crude extract presented antioxidant potential with inhibition percentages of 32.74% ± 0.90 for ABTS and 27.04% ± 1.36 for DPPH. The antimicrobial and antioxidant activities of the crude extract, as well as the antimicrobial property of chitosan-extract films, suggests the potential of these biopolymers for the development of wound healing bandages and new food packaging alternatives.

In vitro effect of the herbicide glyphosate on human blood platelet aggregation and coagulation / Efeito in vitro do herbicida glifosato na agregação plaquetária e coagulação sanguínea em humanos

Glyphosate [N-(phosphonomethyl)-glycine] is a broad-spectrum, non-selective, post-emergence herbicide that is extensively used in agriculture. Published data referring to the effects of this product on human health are contradictory. We showed previously that long-term treatment of rats with low doses of Glyphosate-Biocarb® may induce hepatic histological changes and bleeding without decreasing platelet counts. The aim of the current study was to investigate, in vitro, the effect of glyphosate on human blood platelet aggregation and coagulation. Materials and methods: Platelet aggregation was determined in the platelet-rich plasma using the agents: 6µM-adenosine diphosphate, 6µM-epinephrine and 4µg/mL-collagen. Pretreatment with 500µg/mL glyphosate showed significant hypofunction of the three aggregating agents. The inhibitory effect was dose-dependent at concentrations from 50 to 500 µg/mL. The release of ATP was lower for glyphosate-treated platelets after stimulation by collagen. On the other hand, glyphosate did not promote any inhibitory effects on prothrombin time, thromboplastin time and thrombin time. In conclusion, the results demonstrate that glyphosate promotes changes in the platelet metabolism with an inhibitory effect on primary hemostasis.

O glifosato [N-(phosphonomethyl)-glycine] é um herbicida pós-emergente não seletivo de amplo espectro muito utilizado na agricultura. Dados da literatura referentes aos efeitos desse produto na saúde humana são contraditórios. Em estudos prévios demonstramos que ratos previamente tratados com glifosato apresentavam lesões hepáticas e sangramento sem alterações quantitativas de plaquetas. O objetivo do presente estudo é investigar os efeitos in vitro do glifosato (GP) na agregação plaquetária e coagulação sanguínea em humanos. A agregação plaquetária foi determinada em plasma rico em plaquetas (PRP) usando os agentes adenosina difosfato (ADP) 6µM, epinefrina 6µM e colágeno 4µg/mL. Pré-tratamento com GP 500µg/mL demonstrou significativa hipofunção dos três agentes agregantes. O efeito inibitório foi dose dependente em concentrações de 50-500 µg/mL. Utilizando-se a quantificação de ATP como um índice da capacidade de secreção plaquetária, foi observado diminuição da liberação das plaquetas tratadas com GP. Por outro lado, o GP não promoveu efeito inibidor no tempo de protrombina (TP), tempo de tromboplastina parcial ativada (ATTP) e tempo de trombina (TT). Em conclusão, os resultados demonstram que o GP promove mudanças no metabolismo plaquetário com efeito inibitório na hemostasia primária.

Pentoxifylline for diabetic retinopathy.

BACKGROUND: There is increasing evidence that capillary occlusion plays an important part in the development of diabetic retinopathy. Disaggregants, such as pentoxyfilline may influence the outcome and progression of diabetic retinopathy, but no systematic review of the literature on its efficacy and safety has been published to examine this hypothesis. OBJECTIVES: The aim of the current research was to review the literature in a systematic way in order to assess the effects of pentoxyfilline for diabetic retinopathy in methodologically robust trials. The null hypothesis was that pentoxyfilline has no influence on the progression of diabetic retinopathy or blindness. SEARCH STRATEGY: A systematic search of electronic databases was carried out to identify publications. Relevant papers, written in any language, were accessed and assessed for data. SELECTION CRITERIA: Only randomized controlled clinical trials (RCTs) evaluating the effects of pentoxyfilline in the treatment of diabetic retinopathy were to be included. DATA COLLECTION AND ANALYSIS: Two authors independently assessed studies for inclusion criteria and for risk of bias. MAIN RESULTS: A total of 97 publications were identified by the electronic search and two authors checked the abstracts. Of these, 17 were identified as potentially relevant trials providing information about treatment of patients with diabetic retinopathy using pentoxyfilline and were read in full. Unfortunately, no publication fulfilled our inclusion criteria. AUTHORS' CONCLUSIONS: No sound research to date has examined the treatment of diabetic retinopathy with pentoxyfilline in such a way as to indicate whether this form of intervention has a significant impact on the natural history of this clinical condition. The potential role of this substance in the treatment of diabetic retinopathy remains open to debate, and it is suggested that future research focusing on patient-relevant outcomes takes the opportunity of addressing this important issue directly.

Vitamin C and superoxide dismutase (SOD) for diabetic retinopathy.

BACKGROUND: There is increasing evidence that diabetic retinopathy is caused by the action of free radicals. Radical scavengers like vitamin C and superoxide dismutase (SOD) may influence the outcome and progression of diabetic retinopathy, but no systematic review of the literature has been published to examine this hypothesis. OBJECTIVES: The aim of the current research was to review the literature in a standard systematic way in order to assess the effects of vitamin C and superoxide dismutase on diabetic retinopathy in methodologically robust trials. SEARCH STRATEGY: We tried to obtain studies from computerised searches of MEDLINE, EMBASE, CINAHL, Web of Science and The Cochrane Library. SELECTION CRITERIA: Only randomized clinical trials (RCTs) that evaluated the effect of vitamin C, superoxide dismutase or both in the treatment of diabetic retinopathy were considered. DATA COLLECTION AND ANALYSIS: Two authors independently read all abstracts, titles or both and wanted to assess risk of bias and to perform data extraction. Discrepancies were planned to be resolved by consensus or by the judgement of a third author. MAIN RESULTS: A total of 241 publications were identified by the electronic searches. Of these, 28 were identified as potentially containing information about the treatment of patients with diabetic retinopathy using vitamin C or SOD and were read in full. No trial evaluated the treatment of diabetic retinopathy with vitamin C or SOD. AUTHORS' CONCLUSIONS: No research to date has adequately examined the treatment of diabetic retinopathy with vitamin C or SOD in such a way as to indicate whether this form of intervention has a significant impact on the progress of this clinical condition. The potential role of these substances in the treatment of diabetic retinopathy remains open to debate, and it is suggested that future research focusing on patient-oriented outcomes should address this important issue.

Determinantes e conseqüências da insegurança alimentar no Amazonas: a influência dos ecossistemas / Alimentary insecurity determinants and consequences at Amazonas: ecosystems influences

Nesta revisão foram investigados os principais determinantes da transição epidemiológica, nutricional e do padrão de atividade física vivenciados pelos brasileiros nas últimas três décadas. Constata-se nestas pesquisas a exclusão sistemática da região Norte-rural do novo cenário epidemiológico nacional, atualmente caracterizado pelo declínio da desnutrição infantil e elevação concomitante da obesidade, além da ausência de informações científicas a respeito das condições de saúde, nutrição e sobrevivência das populações de praticamente todos os estados da região Norte. Especificamente para o estado do Amazonas é referida a menor estatura entre as crianças brasileiras, o que evidencia exposição à carências nutricionais de longa duração. Do mesmo modo, a análise evolutiva das pesquisas regionais caracteriza a persistência de um quadro grave de insegurança alimentar. Entretanto, sabe-se que a Amazônia é detentora de uma grande diversidade em peixes e frutos, o que deveria representar uma abundante oferta e utilização de proteína de boa qualidade biológica, calorias, vitaminas, minerais, e assim, viabilizar um adequado padrão de saúde, nutrição e qualidade de vida para sua população. A realidade social e econômica, bem como o quadro de precariedade da saúde e da nutrição da Região contrastam com a sua riqueza em recursos naturais. Para o entendimento deste evidente paradoxo foram investigados os componentes do bioma amazônico quanto à: heterogeneidade, complexidade, fragilidade, interatividade e suas relações com o potencial alimentar da região, bem como seu poder de influência na determinação do binômio saúde versus nutrição para os habitantes dos seus diferentes ecossistemas.

In this review were investigated the principal determinants of the epidemic transition, nutritional and of the pattern of physical activity lived on by Brazilians in the last three decades. It is verified in these researches the systematic exclusion of the rural area from North region of the new national epidemic picture, currently characterized by the decline of the infantile malnutrition and concomitant rise of the obesity, beyond the lack of scientific investigation to be in reference the health, nutrition and survival conditions of the practically all the populations of North region states. Specifically for Amazonas state lesser stature is referred among the Brazilian children that become evident to exposition nutritional needs at long term. The same way, the evolutionary analysis of the regional researches characterizes the persistence of a serious picture of alimentary insecurity. However, it is known that the Amazonia holds a great diversity in fishes and fruits, what should represent an abundant offer and to make use of protein of the highest biological quality, calories, vitamins, minerals, and like this, to make possible an appropriate pattern of health, nutrition and life quality for its population. The social and economical reality, as well as the picture of precariousness of the health and nutritional of the region contradicts with its abundance in natural resources. For the understanding of this obvious paradox the components of the Amazon Bioma were investigated with relation to the: heterogeneity, complexity, fragility, interactivity and their relations with the alimentary potential of the region, as well as its influence ability in the determination of the binomial health versus nutrition for the inhabitants of their several ecosystems.

Physical violence as educational practice

This descriptive and correlational study, carried out at a teaching hospital in 2004, aimed to get to know if, in the context of domestic violence, parents use physical violence as an educational practice. Semistructured interviews were used to characterize the population, and situations of daily family life to identify parents' attitude in the education process. Results: the most vulnerable situation to use physical punishment was disobedience to parents' predetermined orders (40 percent), followed by the situation when the child steals something (31.7 percent). The use of physical violence as a disciplinary practice was significant (p=0.020), associated with unemployment. Forty percent of the population reported they imposed their will on their child, and 57 percent mentioned they had been physically punished by their parents in limit-imposing situations. Knowledge and reflection on factors involving domestic violence are very important to consolidate prevention programs and which could generate a collective consciousness.

Estudio descriptivo y correlacional realizado en un hospital de enseñanza, en 2004, con el objetivo de conocer, si dentro del contexto de violencia doméstica, los padres utilizan la violencia física como práctica educativa. Se utilizó una entrevista semi-estructurada para caracterizar la población y situaciones del cotidiano familiar para identificar la actitud de los padres en el proceso de educación. Resultados: la situación donde hubo gran vulnerabilidad para el uso de la violencia física fue la de desobediencia a órdenes preestablecidas (40 por ciento), acompañada por la situación donde el niño hurta cualquier cosa, con el 31.7 por ciento de los casos. Fue significante (p= 0.020) el uso de la violencia física como práctica disciplinadora, asociada al desempleo. El 40 por ciento de la población afirmó imponer su voluntad sobre el hijo, y el 57 por ciento de haber sido físicamente punido por sus padres en situaciones para imponer límites. Es importante el conocimiento y la reflexión sobre los factores involucrados en la violencia doméstica, para consolidar programas de prevención que sean capaces de generar una conciencia colectiva.

Estudo descritivo e correlacional, realizado em um hospital de ensino, em 2004, objetivando conhecer se, dentro do contexto de violência doméstica, os pais utilizam a violência física como prática educativa. Utilizou-se entrevista semi-estruturada para caracterizar a população, e situações do cotidiano familiar para identificar a atitude dos pais no processo de educação. Resultados: A situação de maior vulnerabilidade para o uso de violência física foi a de desobediência às ordens pré-determinadas (40 por cento), seguida pela situação na qual a criança furta algo, com 31,7 por cento dos casos. Foi significante (p= 0,020) o uso da violência física como prática disciplinadora, associado ao desemprego. Quarenta por cento da população afirma impor sua vontade sobre seu filho, e 57 por cento ter apanhado de seus pais em situações de impor limites. Julga-se importante o conhecimento e a reflexão dos fatores que envolvem a violência doméstica, para alicerçar programas de prevenção e que sejam capazes de gerar uma consciência coletiva.

Physical violence as educational practice.

UNLABELLED: This descriptive and correlational study, carried out at a teaching hospital in 2004, aimed to get to know if, in the context of domestic violence, parents use physical violence as an educational practice. Semistructured interviews were used to characterize the population, and situations of daily family life to identify parents' attitude in the education process. RESULTS: the most vulnerable situation to use physical punishment was disobedience to parents' predetermined orders (40%), followed by the situation when the child steals something (31.7%). The use of physical violence as a disciplinary practice was significant (p=0.020), associated with unemployment. Forty percent of the population reported they imposed their will on their child, and 57% mentioned they had been physically punished by their parents in limit-imposing situations. Knowledge and reflection on factors involving domestic violence are very important to consolidate prevention programs and which could generate a collective consciousness.

[Safe community]. / A comunidade segura.

OBJECTIVE: To analyze the effectiveness of prevention programs for intentional and unintentional injuries, based on safe community principles, in children and adolescents. SOURCES OF DATA: An electronic search was performed in the MEDLINE and LILACS database, corresponding to the period from 2000 to 2005. For prevention of unintentional injuries, we used the following keywords: injury and prevention and community or population and intervention. For prevention of intentional injuries, we used the following keywords: violence and prevention and community or population and intervention; the age range from zero to 18 years was used as a threshold. The inclusion criteria were: original articles which evaluated the effectiveness of interventions using more than one strategy, involving a whole community or group, published in Portuguese, English or Spanish. SUMMARY OF THE FINDINGS: 152 studies were obtained as a result. Considering the inclusion criteria, five articles were found on unintentional injury prevention, one about intentional injury prevention and one paper addressing these two issues. All studies analyzed demonstrate benefits to children and adolescents, but in different proportions. CONCLUSIONS: A restricted number of programs using the safe community concept were found. The investigations analyzed in this study presented positive results. Increased efforts to further develop this evidence are still needed, respecting local characteristics, and developing evaluation indicators that allow for a better comparison between different studies.

A comunidade segura / Safe community

OBJETIVO: Analisar a efetividade de programas de prevenção de injúrias intencionais e não intencionais, em crianças e adolescentes, embasados nos princípios da comunidade segura. FONTES DE DADOS: Pesquisa eletrônica nas bases de dados MEDLINE e LILACS, correspondendo ao período de 2000 a 2005. Para prevenção de injúrias não intencionais, usaram-se os descritores: injury and prevention and community or population and intervention; para prevenção de injúrias intencionais, violence and prevention and community or population and intervention; foi tomada como limite a faixa etária de 0 a 18 anos. Critérios de inclusão: artigos originais, que estudaram a efetividade de intervenções utilizando mais de uma estratégia e abrangendo toda uma comunidade ou grupo, abordando injúrias intencionais e não intencionais, publicados em português, inglês ou espanhol. SíNTESE DOS DADOS: Foram obtidos 152 estudos como resultado. Considerando os critérios de inclusão, foram encontrados cinco artigos sobre prevenção de injúrias não intencionais, um sobre prevenção de injúrias intencionais e um artigo que aborda as duas questões. As pesquisas analisadas evidenciam benefícios para crianças e adolescentes, em diferentes proporções. CONCLUSÕES: Os programas que trabalham com o conceito de comunidade segura ainda são em número restrito. As investigações analisadas neste estudo apresentam resultados positivos. É preciso aumentar esforços para o desenvolvimento dessa base de evidência, respeitando as características locais, desenvolvendo indicadores de avaliação que permitam melhor comparação entre diferentes trabalhos.

HIV-1 binding and neutralizing antibodies of injecting drug users.

Previous studies have demonstrated a stronger seroreactivity against some synthetic peptides responsible for inducing neutralizing antibodies in injecting drug users (IDU) compared to that of individuals sexually infected with HIV-1 (S), but the effectiveness in terms of the neutralizing ability of these antibodies has not been evaluated. Our objective was to study the humoral immune response of IDU by determining the specificity of their antibodies and the presence of neutralizing antibodies. The neutralization capacity against the HIV-1 isolate MN (genotype B), the primary HIV-1 isolate 95BRRJ021 (genotype F), and the seroreactivity with peptides known to induce neutralizing antibodies, from the V2 and V3 loops of different HIV-1 subtypes, were analyzed. Seroreactivity indicates that IDU plasma are more likely to recognize a broader range of peptides than S plasma, with significantly higher titers, especially of V3 peptides. Similar neutralization frequencies of the MN isolate were observed in plasma of the IDU (16/47) and S (20/60) groups in the 1:10 dilution. The neutralization of the 95BRRJ021 isolate was more frequently observed for plasma from the S group (15/23) than from the IDU group (15/47, P = 0.0108). No correlation between neutralization and seroreactivity with the peptides tested was observed. These results suggest that an important factor responsible for the extensive and broad humoral immune response observed in IDU is their infection route. There was very little difference in neutralizing antibody response between the IDU and S groups despite their differences in seroreactivity and health status.

HIV-1 binding and neutralizing antibodies of injecting drug users

Previous studies have demonstrated a stronger seroreactivity against some synthetic peptides responsible for inducing neutralizing antibodies in injecting drug users (IDU) compared to that of individuals sexually infected with HIV-1 (S), but the effectiveness in terms of the neutralizing ability of these antibodies has not been evaluated. Our objective was to study the humoral immune response of IDU by determining the specificity of their antibodies and the presence of neutralizing antibodies. The neutralization capacity against the HIV-1 isolate MN (genotype B), the primary HIV-1 isolate 95BRRJ021 (genotype F), and the seroreactivity with peptides known to induce neutralizing antibodies, from the V2 and V3 loops of different HIV-1 subtypes, were analyzed. Seroreactivity indicates that IDU plasma are more likely to recognize a broader range of peptides than S plasma, with significantly higher titers, especially of V3 peptides. Similar neutralization frequencies of the MN isolate were observed in plasma of the IDU (16/47) and S (20/60) groups in the 1:10 dilution. The neutralization of the 95BRRJ021 isolate was more frequently observed for plasma from the S group (15/23) than from the IDU group (15/47, P = 0.0108). No correlation between neutralization and seroreactivity with the peptides tested was observed. These results suggest that an important factor responsible for the extensive and broad humoral immune response observed in IDU is their infection route. There was very little difference in neutralizing antibody response between the IDU and S groups despite their differences in seroreactivity and health status.

Human immunodeficiency virus type 1 (HIV-1) genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing highly active antiretroviral therapy.

In order to assess the human immunodeficiency virus type 1 (HIV-1) drug resistance mutation profiles and evaluate the distribution of the genetic subtypes in the state of Rio de Janeiro, Brazil, blood samples from 547 HIV-1 infected patients failing antiretroviral (ARV) therapy, were collected during the years 2002 and 2003 to perform the viral resistance genotyping at the Renageno Laboratory from Rio de Janeiro (Oswaldo Cruz Foundation). Viral resistance genotyping was performed using ViroSeq Genotyping System (Celera Diagnostic-Abbott, US). The HIV-1 subtyping based on polymerase (pol) gene sequences (protease and reverse transcriptase-RT regions) was as follows: subtype B (91.2%), subtype F (4.9%), and B/F viral recombinant forms (3.3%). The subtype C was identified in two patients (0.4%) and the recombinant CRF_02/AG virus was found infecting one patient (0.2%). The HIV-1 genotyping profile associated to the reverse transcriptase inhibitors has shown a high frequency of the M184V mutation followed by the timidine-associated mutations. The K103N mutation was the most prevalent to the non-nucleoside RT inhibitor and the resistance associated to protease inhibitor showed the minor mutations L63P, L10F/R, and A71V as the more prevalent. A large proportion of subtype B was observed in HIV-1 treated patients from Rio de Janeiro. In addition, we have identified the circulation of drug-resistant HIV-1 subtype C and are presenting the first report of the occurrence of an African recombinant CRF_02/AG virus in Rio de Janeiro, Brazil. A clear association between HIV-1 subtypes and protease resistance mutations was observed in this study. The maintenance of resistance genotyping programs for HIV-1 failing patients is important to the management of ARV therapies and to attempt and monitor the HIV-1 subtype prevalence in Brazil.

Human immunodeficiency virus type 1 (HIV-1) genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing highly active antiretroviral therapy

In order to assess the human immunodeficiency virus type 1 (HIV-1) drug resistance mutation profiles and evaluate the distribution of the genetic subtypes in the state of Rio de Janeiro, Brazil, blood samples from 547 HIV-1 infected patients failing antiretroviral (ARV) therapy, were collected during the years 2002 and 2003 to perform the viral resistance genotyping at the Renageno Laboratory from Rio de Janeiro (Oswaldo Cruz Foundation). Viral resistance genotyping was performed using ViroSeqTM Genotyping System (Celera Diagnostic-Abbott, US). The HIV-1 subtyping based on polymerase (pol) gene sequences (protease and reverse transcriptase-RT regions) was as follows: subtype B (91.2 percent), subtype F (4.9 percent), and B/F viral recombinant forms (3.3 percent). The subtype C was identified in two patients (0.4 percent) and the recombinant CRF_02/AG virus was found infecting one patient (0.2 percent). The HIV-1 genotyping profile associated to the reverse transcriptase inhibitors has shown a high frequency of the M184V mutation followed by the timidine-associated mutations. The K103N mutation was the most prevalent to the non-nucleoside RT inhibitor and the resistance associated to protease inhibitor showed the minor mutations L63P, L10F/R, and A71V as the more prevalent. A large proportion of subtype B was observed in HIV-1 treated patients from Rio de Janeiro. In addition, we have identified the circulation of drug-resistant HIV-1 subtype C and are presenting the first report of the occurrence of an African recombinant CRF_02/AG virus in Rio de Janeiro, Brazil. A clear association between HIV-1 subtypes and protease resistance mutations was observed in this study. The maintenance of resistance genotyping programs for HIV-1 failing patients is important to the management of ARV therapies and to attempt and monitor the HIV-1 subtype prevalence in Brazil.

A memória dos idosos em tarefas complexas / Aging and memory in complex tasks

Dois experimentos compararam o desempenho de jovens e idosos nos testes de recuperação livre, recuperação com pista extra-lista e recuperação com pista intra-lista. No primeiro experimento, foi avaliada a influência da complexidade da tarefa, do tempo de apresentação dos estímulos e da faixa etária sobre a recuperação livre de palavras apresentadas no contexto de sentenças. No segundo, jovens e idosos foram comparados para verificar os efeitos da complexidade da tarefa, do tipo de teste, do tamanho do conjunto do alvo e da força associativa da pista sobre a memória de palavras no contexto de sentenças do teste de recuperação com pista extra-lista e intra-lista. Os resultados mostraram uma redução na evocação em função do aumento da idade dos participantes. Também indicaram que a magnitude da diferença variou, dependendo do tipo de teste de memória aplicado e da presença de contexto relacionado ao alvo durante a codificação.

A memória dos idosos em tarefas complexas / Aging and memory in complex tasks

Dois experimentos compararam o desempenho de jovens e idosos nos testes de recuperação livre, recuperação com pista extra-lista e recuperação com pista intra-lista. No primeiro experimento, foi avaliada a influência da complexidade da tarefa, do tempo de apresentação dos estímulos e da faixa etária sobre a recuperação livre de palavras apresentadas no contexto de sentenças. No segundo, jovens e idosos foram comparados para verificar os efeitos da complexidade da tarefa, do tipo de teste, do tamanho do conjunto do alvo e da força associativa da pista sobre a memória de palavras no contexto de sentenças do teste de recuperação com pista extra-lista e intra-lista. Os resultados mostraram uma redução na evocação em função do aumento da idade dos participantes. Também indicaram que a magnitude da diferença variou, dependendo do tipo de teste de memória aplicado e da presença de contexto relacionado ao alvo durante a codificação.(AU)

Causas consequências e formas de prevenção da violência doméstica contra a criança e o adolescente / Causes consequences and ways of preventing domestic violence against the child and the adolescent

Este artigo objetiva oferecer subsídios para reflexão sobre alguns aspectos históricos da violência doméstica contra criança e adolescente, que contribuíram e contribuem para perpetuar até os dias atuais, a idéia da utilização da violência como prática educativa, como força de opressão e punição na criação dos filhos...