ABSTRACT
An improved direct agglutination test (DAT) is assessed for the detection of Leishmania infantum antibodies in man and in the canine reservoir in Southern France. The test performance in 32 human visceral leishmaniasis patients was in agreement with the parasitological diagnosis and the immunofluorescence (IFAT) results. In six patients diagnosed earlier as kala-azar cases, both DAT and IFAT revealed negative results. The specificity of the DAT was reproduced in this study by the absence of cross-reactions with sera from other patients. In the dog reservoir, the DAT results were compared with those obtained by IFAT, the enzyme-linked immunosorbent assay (ELISA), cross-over electrophoresis and the latex agglutination test. All 34 dogs with parasitologically positive lymph node aspirates also had positive DAT titres (less than or equal to 1:640); the sensitivity in the other four tests was relatively low. Positive DAT and IFAT results were found in 16 unconfirmed cases of canine leishmaniasis, most probably due to prepatent L. infantum infections.
