Optimization of spermatozoa analysis in mice: A comprehensive protocol.

Sperm quality is critical to predict reproductive alterations caused by immunological factors or toxicant agents. Yet, no detailed protocol has been published focusing on analyses of sperm parameters in mice. Our aim was to evaluate the most efficient diluent for mice sperm analyses and to optimize the sperm morphology classification, through the comparison of different staining methods. The diluents assessed were PBS (baseline), HTF, DMEM, 1 % BSA in PBS and 9 % skimmed powdered milk diluted in PBS. Spermatozoa were evaluated for vitality, motility, and morphology, smears were stained with Papanicolaou, HE, Giemsa, and Rapid staining. Sperm vitality and total motility reached better scores in milk based and DMEM diluents. HE raised up as an effective option since its combination with any of the diluents we tested, resulted in a fair staining, which was appropriated to evaluate mice spermatozoa. Finally, based on WHO manual, we have updated the current morphological classification for mice sperm, since we have detailed the head defects as well as included midpiece and tail defects on it. Taken together, we presented a useful, low cost, and reliable method to assess sperm morphology that could be employed worldwide by laboratories dedicated to study reproductive biology on mice model.

Systematic review of literature to evaluate global distribution of species of the Sporothrix genus stored in culture collections.

Introduction: Sporotrichosis is a subcutaneous mycosis caused by fungi of the genus Sporothrix sp. Phenotypic and genotypic differences have been associated with their geographic distribution, virulence, or clinical manifestation of sporotrichosis. In the past decade, the interest in identifying species of the Sporothrix sp. has been increasing, due to its epidemiological importance and, in consequence, is important to know how to preserve them for future studies, in culture collection. Aims: The purposes of this study were to analyze the global distribution of environmental isolates and/or causal agents of sporotrichosis identified by polyphasic taxonomy, with mandatory use of molecular identification, and to evaluate the percentages and distribution of isolates stored in culture collections. Methods: A systematic review of articles on animal and human sporotrichosis and/or environmental isolation of the fungus, from 2007 to 2023, was done. Results: Our results demonstrated that, S. globosa, S. schenckii, and S. brasiliensis were the most identified species. With respect to the deposit and maintenance of species, we observed that only 17% of the strains of Sporothrix sp. isolated in the world are preserved in a culture collection. Conclusions: This systematic review confirmed a difficulty in obtaining the frequency of Sporothrix species stored in culture collection and insufficient data on the molecular identification mainly of animal sporotrichosis and isolation of Sporothrix sp. in environmental samples.

Field Hip Stability Isometric Test (F-HipSIT): Reliability of Assessing the Hip Posterolateral Muscle Strength in Sports Settings.

CONTEXT: The Hip Stability Isometric Test (HipSIT) is commonly employed in clinical settings for evaluating the hip posterolateral muscle strength. In this study, we introduced the "Field Hip Stability Isometric Test" (F-HipSIT) and assessed the intrarater and interrater reliability of this strength assessment specifically designed for sports settings. DESIGN: Reliability study. METHODS: Two independent raters (A and B) went to athletes' training facilities to conduct 2 sessions of F-HipSIT spaced at least 1 week apart. The average peak force value from 3 valid attempts of each leg was recorded and normalized by the participant's body mass for statistical analysis. RESULTS: Thirty male and 30 female amateur athletes took part in this study. Rater A obtained similar values in the first (0.39 [0.05] and 0.44 [0.07] kg·f/kg) and second (0.39 [0.06] and 0.45 [0.07] kg·f/kg) testing days for men and women, respectively. Rater B also found similar values in the first (0.35 [0.06] and 0.42 [0.08] kg·f/kg) and second (0.36 [0.06] and 0.45 [0.08] kg·f/kg) testing days for men and women, respectively. Excellent intrarater intraclass correlation coefficient (ICC) values were found for men (ICC = .922) and women (ICC = .930), with coefficient of variation of 6% to 8% and minimal detectable change of 0.06 to 0.10 kg·f/kg. The F-HipSIT presented good interrater reliability for men (ICC = .857) and women (ICC = .868), with coefficient of variation of 5% and minimal detectable change of 0.05 to 0.06 kg·f/kg. CONCLUSION: The F-HipSIT intrarater and interrater reliability among male and female recreational athletes supports this field test as a quick and convenient screening tool to monitor hip posterolateral muscle strength in sports settings.

Ebselen: A Promising Repurposing Drug to Treat Infections Caused by Multidrug-Resistant Microorganisms.

Bacterial multiresistance to drugs is a rapidly growing global phenomenon. New resistance mechanisms have been described in different bacterial pathogens, threatening the effective treatment of even common infectious diseases. The problem worsens in infections associated with biofilms because, in addition to the pathogen's multiresistance, the biofilm provides a barrier that prevents antimicrobial access. Several "non-antibiotic" drugs have antimicrobial activity, even though it is not their primary therapeutic purpose. However, due to the urgent need to develop effective antimicrobials to treat diseases caused by multidrug-resistant pathogens, there has been an increase in research into "non-antibiotic" drugs to offer an alternative therapy through the so-called drug repositioning or repurposing. The prospect of new uses for existing drugs has the advantage of reducing the time and effort required to develop new compounds. Moreover, many drugs are already well characterized regarding toxicity and pharmacokinetic/pharmacodynamic properties. Ebselen has shown promise for use as a repurposing drug for antimicrobial purposes. It is a synthetic organoselenium with anti-inflammatory, antioxidant, and cytoprotective activity. A very attractive factor for using ebselen is that, in addition to potent antimicrobial activity, its minimum inhibitory concentration is very low for microbial pathogens.

Genotoxic and antiproliferative properties of Endopleura uchi bark aqueous extract.

The bark extract from Endopleura uchi has been widely used in traditional medicine to treat gynecological-related disorders, diabetes, and dyslipidemias albeit without scientific proof. In addition, E. uchi bark extract safety, especially regarding mutagenic activities, is not known. The aim of this study was to determine the chemical composition, antitumor, and toxicological parameters attributed to an E. uchi bark aqueous extract. The phytochemical constitution was assessed by colorimetric and chromatographic analyzes. The antiproliferative effect was determined using sulforhodamine B (SRB) assay using 4 cancer cell lines. Cytotoxic and genotoxic activities were assessed utilizing MTT and comet assays, respectively, while mutagenicity was determined through micronucleus and Salmonella/microsome assays. The chromatographic analysis detected predominantly the presence of gallic acid and isoquercitrin. The antiproliferative effect was more pronounced in human colon adenocarcinoma (HT-29) and human breast cancer (MCF-7) cell lines. In the MTT assay, the extract presented an IC50 = 39.1 µg/ml and exhibited genotoxic (comet assay) and mutagenic (micronucleus test) activities at 20 and 40 µg/ml in mouse fibroblast cell line (L929) and mutagenicity in the TA102 and TA97a strains in the absence of S9 mix. Data demonstrated that E. uchi bark possesses bioactive compounds which exert cytotoxic and genotoxic effects that might be associated with its antitumor potential. Therefore, E. uchi bark aqueous extract consumption needs to be approached with caution in therapeutic applications.

Frederic Lewy: how the two World Wars changed his life, work, and name.

In 1912, Friedrich Lewy described the inclusion bodies present in Parkinson disease and in Lewy body dementia. Throughout his life, Lewy fought in two wars - on opposite sides. He was born in Berlin in a Jewish family, and served in the German Army in World War I. In the following years, on many occasions he had to change his line of research due to Nazi persecution. Lewy became a naturalized American, changed his name to Frederic Henry Lewey, and served in the US Army as a lieutenant colonel. Lewy died in 1950 and never used the famous eponym in his papers.

Em 1912, Friedrich Lewy descreveu os corpos de inclusão presentes na doença de Parkinson e na demência com corpos de Lewy. Ao longo sua vida, Lewy lutou em duas guerras, em lados opostos. Ele nasceu em Berlin em uma família de origem judaica e serviu no Exército alemão na Primeira Guerra Mundial. Nos anos seguintes, passou muitas vezes teve de mudar sua linha de pesquisa devido à perseguição Nazista. Lewy naturalizou-se americano, mudou seu nome para Frederic Henry Lewey, e serviu no Exército americano como tenente-coronel. Lewy morreu em 1950 e nunca usou o famoso epônimo em seus artigos.

The upcoming European Soil Monitoring Law: An effective instrument for the protection of terrestrial ecosystems?

Soils are a precious resource consistently placed under several threats and urgently in need of protection within a regulatory framework at the European level. Soils are central to the provision of environmental services as well as human existence on earth. The need to protect soil has been identified by several recent European strategies and fortunately, a specific European regulation for soil protection is on the way-the European Soil Monitoring Law (formerly: Soil Health Law). However, efforts need to ensure that the upcoming Soil Monitoring Law closes gaps between existing regulations for chemicals and acknowledges current European strategies for environmental protection and sustainability. This brief communication started from a fruitful discussion among SETAC Global Soils Interest Group members on a recent public consultation on the newly proposed Soil Monitoring Law of the European Commission and highlights critical points focusing on the chemical pollution of soils. We emphasize urgent needs such as the essential definition of a "healthy state" of soils; the implementation of a suitable set of indicators and quality standards for the description of physical, chemical, and biological states of soils; the enforcement of the "polluter-pays" principle; and the establishment of a Europe-wide monitoring program. Results from monitoring need to be fed back into regulatory frameworks, including the regulation of chemicals. Guidance documents for the risk assessment of chemicals are outdated and need to be updated. Finally, actions need to be taken to foster healthy soils, stop biodiversity decline, and ensure the functioning of ecosystem services for future generations. Integr Environ Assess Manag 2024;20:316-321. © 2023 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).

Frederic Lewy: how the two World Wars changed his life, work, and name / Frederic Lewy: como as duas Guerras Mundiais mudaram sua vida, trabalho e nome

Abstract In 1912, Friedrich Lewy described the inclusion bodies present in Parkinson disease and in Lewy body dementia. Throughout his life, Lewy fought in two wars - on opposite sides. He was born in Berlin in a Jewish family, and served in the German Army in World War I. In the following years, on many occasions he had to change his line of research due to Nazi persecution. Lewy became a naturalized American, changed his name to Frederic Henry Lewey, and served in the US Army as a lieutenant colonel. Lewy died in 1950 and never used the famous eponym in his papers.

Resumo Em 1912, Friedrich Lewy descreveu os corpos de inclusão presentes na doença de Parkinson e na demência com corpos de Lewy. Ao longo sua vida, Lewy lutou em duas guerras, em lados opostos. Ele nasceu em Berlin em uma família de origem judaica e serviu no Exército alemão na Primeira Guerra Mundial. Nos anos seguintes, passou muitas vezes teve de mudar sua linha de pesquisa devido à perseguição Nazista. Lewy naturalizou-se americano, mudou seu nome para Frederic Henry Lewey, e serviu no Exército americano como tenente-coronel. Lewy morreu em 1950 e nunca usou o famoso epônimo em seus artigos.

Sofrimento psíquico de pessoas em situação de rua: cotidiano de quem sofre e estratégias para o cuidado / Psychological suffering of homeless people: daily life of those who suffer and strategies for care / Sufrimiento de las personas sin hogar: cotidiano de quien sufre y estrategias de cuidado

Introdução: Apesar de haver uma política de saúde pública implementada e em consolidação no Brasil, o acesso aos direitos e serviços de cuidado ainda é um desafio, sobretudo em saúde mental, para a população em situação de rua. Objetivo: O presente estudo buscou melhor compreender o cotidiano da população em situação de rua com sofrimento psíquico grave e as estratégias de cuidado em saúde mental para ela voltados. Métodos: Parte-se de uma aproximação gradativa e mediada pela equipe do Consultório na Rua de Santos, entrevistando trabalhadores e usuários do serviço, além da observação participante em ações desenvolvidas no período de dois meses. Resultados: Os achados afirmam a força das tecnologias leves em saúde como estratégia prioritária de cuidado, além de destacarem a centralidade do território na leitura da realidade e na proposição de ações de cuidado, especialmente, para as situações em que o mesmo se configura como casa. Destaca-se a escuta e a fala enquanto atividades humanas, norteando o processo de atenção em terapia ocupacional. Conclusão: Salienta-se a composição das equipes de Consultório na Rua com terapeutas ocupacionais, visto suas contribuições no que se refere à leitura e à intervenção junto ao cotidiano das pessoas atendidas. Com isso, pode-se construir uma transformação das cenas, envolvendo pessoas e territórios, trabalhando na defesa dos direitos de cidadania e na determinação social das subjetividades, além da necessidade de modificação das noções de território, morar e habitar, sobretudo, no que diz respeito à população em situação de rua. (AU)

Introduction: Introduction: Although there is an integrated public health policy in consolidation in Brazil, access to rights and care services is still a challenge, especially in mental health, for the homeless population. Objective: The present study sought to better understand the daily life of the homeless population with severe psychological distress and the mental health care strategies aimed at them. Methods: We started with a gradual approach, mediated by the Consultório na Rua de Santos team, interviewing workers and users of the service, in addition to participant observation in actions developed over a period of two months. Results: Our findings affirm the strength of health technologies as a priority care strategy, in addition to highlighting the centrality of the territory in reading reality and proposing care actions, especially for situations in which it is configured as a home. We highlight listening and speech as human activities, guiding the care process in occupational therapy. Conclusion: We emphasize the composition of the Consultório na Rua teams with occupational therapists, given their contributions with regard to reading and intervention in the daily lives of the people assisted, being able to construct a transformation of the scenes, involving people and territories, working in defense of the citizenship rights and in the social experience of subjectivities, in addition to the need to modify the notions of territory, living and dwelling, especially regarding the homeless population. (AU)

Introducción: A pesar de que existe una política pública integrada de salud en consolidación en Brasil, el acceso a los derechos y servicios de atención sigue siendo un desafío, especialmente en salud mental, para la población sin hogar. Objetivo: El presente estudio buscó comprender mejor el cotidiano de la población sin hogar con sufrimiento psíquico severo y las estrategias de atención en salud mental dirigidas a ellos. Métodos: Partimos de un abordaje gradual, mediado por el equipo de Consultório na Rua de Santos, entrevistando a trabajadores y usuarios del servicio, además de observación participante en acciones desarrolladas en un período de dos meses. Resultados: Nuestros hallazgos afirman la fortaleza de las tecnologías de la salud como estrategia de atención prioritaria, además de resaltar la centralidad del territorio en la lectura de la realidad y la propuesta de acciones de cuidado, especialmente para situaciones en las que se configura como hogar. Destacamos la escucha y el habla como actividades humanas, orientando el proceso de atención en terapia ocupacional. Conclusión: Destacamos la composición de los equipos de lo Consultório na Rua con terapeutas ocupacionales, dados sus aportes en cuanto a la lectura y la intervención en el cotidiano de las personas atendidas, pudiendo construir una transformación de los escenarios, involucrando personas y territorios, trabajando en defensa de los derechos ciudadanos y en la vivencia social de las subjetividades, además de la necesidad de modificar las nociones de territorio, habitar y habitar, especialmente en lo que se refiere a la población sin hogar. (AU)

Effects of glyphosate on earthworms: From fears to facts.

Glyphosate is the most widely applied herbicide worldwide, contaminating water, soils, and living organisms. Earthworms are emblematic soil organisms used as indicators of soil quality, but knowledge about the impacts of glyphosate and glyphosate-based herbicides (GBH) on these key soil organisms is scattered. Here, we examine this knowledge in detail to answer four questions: (1) Which endpoint is the most sensitive when assessing the effects of glyphosate or GBH in earthworms? (2) Which is most toxic to earthworms: glyphosate or GBH? (3) Are glyphosate and GBH harmful to earthworms when used at the recommended application dose? (4) What are the interactions between glyphosate or GBH and other chemicals in earthworms? The results indicate that a weak legislation led to improper assessment of the ecotoxicity of glyphosate during the last renewal in 2017. Our findings also highlighted that negative effects can occur in earthworms at the recommended application rate, although not after only a single application or when considering only the mortality of adult individuals. However, under more realistic conditions, that is, when assessing sensitive endpoints (e.g., reproduction, growth) and using species present in the field, after several applications per year, the negative effects of glyphosate or GBH on earthworms were observed at the subindividual, individual, population, and community levels, as well as on earthworm-mediated functions. Our recommendations are as follows: (i) competent agencies should collect more information on the toxicity of these compounds to earthworms before the next renewal deadline, with emphasis on the use of the updated legislation on the topic, and (ii) scientists should increase research on the effects of these herbicides on soil invertebrate species, with emphasis on earthworms, using guideline tests and obtain data from long-term field testing. Integr Environ Assess Manag 2023;00:1-7. © 2023 SETAC.

ANALYSIS OF THE TRACKING INITIATIVES OF COLORECTAL CANCER IN BRAZIL.

BACKGROUND: Colorectal cancer (CRC) is an important public health problem, as it represents the world's third most diagnosed neoplasm and the fourth cause of mortality. Its prevention can be divided into primary, secondary, demonstrated by tracking techniques, and tertiary, which consists of cancer diagnosis in symptomatic patients. Despite presenting a high incidence, the mortality rates decreased in the past two decades in developed countries, while the opposite happened in underdeveloped countries. That is attributed to the increase of colorectal cancer tracking programs in developed countries, which allows the precocious diagnosis and treatment of precancerous injuries and CRC. In that manner, the American Cancer Society divides the secondary tracking methods in exams based on feces samples and visual analysis of the colon and rectum, indicating its initiation starting at 45 years old in lower-risk patients. OBJECTIVE: Verify in an analytical way the actions of colorectal cancer tracking held in Brazil, as to evaluate the necessity of implementation of a national tracking program of CRC. METHODS: The methodology was based on a descriptive-quantitative secondary study that correlated the incidence of CRC, its morbidity and mortality, and the impact of the precocious tracking programs. It included activities not reported in medical literature through personal contacts with coordinators of regional programs to compare with the existent data in the literature. It was used as a variable for the tracking strategies the exams held, and their respective results. RESULTS: It described nine programs held in different regions in Brazil, using two screening methods for CRC: a noninvasive method that consists of the research of blood hidden in feces, being the majority through the FIT method, and an invasive method, having the colonoscopy as its representant. These initiatives were effective in the detection of early forms of this disease. CONCLUSION: Despite the existence of several private tracking programs and the broad divulgation of the importance of the tracking and the early diagnosis of colorectal cancer, it was demonstrated that Brazil lacks a national program that patronizes the tracking methods, which reflects in the major prevalence of late diagnosis in the population.

Análise das iniciativas de rastreamento do câncer colorretal no Brasil / Analysis of the tracking initiatives of colorectal cancer in brazil

ABSTRACT Background: Colorectal cancer (CRC) is an important public health problem, as it represents the world's third most diagnosed neoplasm and the fourth cause of mortality. Its prevention can be divided into primary, secondary, demonstrated by tracking techniques, and tertiary, which consists of cancer diagnosis in symptomatic patients. Despite presenting a high incidence, the mortality rates decreased in the past two decades in developed countries, while the opposite happened in underdeveloped countries. That is attributed to the increase of colorectal cancer tracking programs in developed countries, which allows the precocious diagnosis and treatment of precancerous injuries and CRC. In that manner, the American Cancer Society divides the secondary tracking methods in exams based on feces samples and visual analysis of the colon and rectum, indicating its initiation starting at 45 years old in lower-risk patients. Objective: Verify in an analytical way the actions of colorectal cancer tracking held in Brazil, as to evaluate the necessity of implementation of a national tracking program of CRC. Methods: The methodology was based on a descriptive-quantitative secondary study that correlated the incidence of CRC, its morbidity and mortality, and the impact of the precocious tracking programs. It included activities not reported in medical literature through personal contacts with coordinators of regional programs to compare with the existent data in the literature. It was used as a variable for the tracking strategies the exams held, and their respective results. Results: It described nine programs held in different regions in Brazil, using two screening methods for CRC: a noninvasive method that consists of the research of blood hidden in feces, being the majority through the FIT method, and an invasive method, having the colonoscopy as its representant. These initiatives were effective in the detection of early forms of this disease. Conclusion: Despite the existence of several private tracking programs and the broad divulgation of the importance of the tracking and the early diagnosis of colorectal cancer, it was demonstrated that Brazil lacks a national program that patronizes the tracking methods, which reflects in the major prevalence of late diagnosis in the population.

RESUMO Contexto: O câncer colorretal (CCR) é um importante problema de saúde pública, uma vez que representa a terceira neoplasia mais diagnosticada no mundo e a quarta causa de mortalidade. Sua prevenção pode ser dividida em primária, secundária, demonstrada pelas técnicas de rastreamento e terciária, que consiste no diagnóstico de câncer em pacientes sintomáticos. Apesar de apresentar uma alta incidência, as taxas de mortalidade diminuíram nas últimas duas décadas nos países desenvolvidos, enquanto o contrário ocorreu em países subdesenvolvidos, o que atribui-se a elevação dos programas de rastreamento colorretal nesses países, de modo que permite o diagnóstico precoce e o tratamento de lesões pré-cancerosas e do CCR. Desse modo, a American Cancer Society divide os métodos de rastreio secundário em testes baseados em amostras de fezes e exames visuais do cólon e reto, indicando-se seu início a partir dos 45 anos de idade, em pacientes de baixo risco. Objetivo: Verificar de forma analítica as ações de rastreamento do câncer colorretal realizadas no Brasil, de modo a avaliar a necessidade da implementação de um programa nacional de rastreamento do CCR. Métodos: A metodologia baseou-se em um estudo secundário quantitativo descritivo que correlacionou a incidência do CCR e sua morbimortalidade e o impacto dos programas de rastreamento precoce. Foram incluídas atividades não relatadas na literatura médica através de contatos pessoais com coordenadores de programas regionais, de modo a comparar com os dados existentes na literatura. Utilizou-se como variáveis as estratégias de triagem, os exames realizados e seus respectivos resultados. Resultados: Foram descritos nove programas realizados em diversas regiões do Brasil utilizando dois métodos de rastreio para o CCR, um não invasivo que consiste na pesquisa de sangue oculto nas fezes, sendo a maioria pelo método FIT; e outro invasivo, tendo a colonoscopia como representante. Essas iniciativas foram efetivas na detecção de formas precoces desta doença. Conclusão: Apesar da existência de diversos programas de rastreamento de início privativo e a ampla divulgação da importância do rastreamento e diagnóstico precoce do CCR, foi demonstrado que o Brasil carece de um programa nacional que padronize as formas de rastreio, o que reflete na maior prevalência de diagnósticos tardios na população.

Avazum app usability testing. / Teste de usabilidade do aplicativo Avazum.

PURPOSE: To verify the effectiveness, efficiency, and satisfaction in the usability test of the tinnitus assessment application. METHODS: This is a descriptive usability test study that assessed the satisfaction, effectiveness, and efficiency of the application. The test was carried out virtually via Google Forms. First, the participants received instructions on how to access and use Avazum, through texts and illustrative images. Afterward, the users used the application, observing its functions and usability. Next, the participants answered the usability questionnaire. Statistical analysis was performed using the Statistical Package for the Social Sciences, version 21.0, to perform descriptive analysis such as frequency, percentages of data in the System Usability Questionnaire (SUS), and analysis in the Net Promoter Score (NPS). RESULTS: Thus, the overall mean SUS score was consistent with satisfactory usability, which implies that the application does not present serious usability problems. Also, the overall NPS percentage indicated very good user satisfaction, with a good percentage of promoting users. As far as effectiveness is concerned, it was found that Avazum reached its goals, besides being efficient, as it uses clear language and is comfortably used. CONCLUSION: Avazum obtained very good satisfaction from users, in addition to achieving its goals. It proved to be effective, has clear language, and is comfortably used, adducing efficiency in the multidisciplinary evaluation of tinnitus.

OBJETIVO: Verificar a efetividade, eficiência e satisfação no teste de usabilidade para o aplicativo de avaliação do zumbido. MÉTODO: Trata-se de um estudo descritivo de teste de usabilidade, no qual será avaliado satisfação, eficácia e eficiência do aplicativo. O teste foi realizado virtualmente por meio de um formulário do Google Forms. Primeiramente, os participantes receberam as instruções de acesso e uso do avazum através de textos e imagens ilustrativas. Logo após, os usuários fizeram o uso do aplicativo, observando suas funções e usabilidade. Em seguida, os participantes responderam o questionário de usabilidade. A análise estatística foi realizada no software Statistics Package for the Social Sciences (SPSS) versão 21.0 para realizar a análise descritiva como frequência, percentagens dos dados do System Usability Questionnaire (SUS), além de análise do score do Net Promoter Score (NPS). RESULTADOS: Sendo assim, a média geral do score do SUS foi condizente com score de usabilidade satisfatório, o que implica dizer que o aplicativo não apresenta graves problemas de usabilidade, além da porcentagem geral NPS indicar uma satisfação muito boa dos usuários, com bom percentual de usuários promotores. No que diz respeito sobre a efetividade, foi possível observar que o Avazum atinge os objetivos propostos, além de ser eficiente, pois apresenta uma linguagem clara e confortabilidade durante o uso. CONCLUSÃO: O Avazum obteve satisfação muito boa dos usuários, além de atingir os objetivos propostos, tornando-o efetivo, apresentou uma linguagem clara e confortabilidade durante o uso, aduzindo eficiência na avaliação multidisciplinar do zumbido.

Relationship between adherence to dietary treatment and binge-eating disorder in obese patients / Relación entre la adherencia al tratamiento dietético y el trastorno por atracón en pacientes con obesidad

Introduction: the binge eating disorder (BED) is the eating disorder that most affects the obese population, and BED is an aspect that possiblyinterferes with the rate of adherence to nutritional treatment, which is also low in individuals with chronic non-communicable disease. Objective: to examine the relationship between adherence to dietary treatment and BED presence in obese individuals.Methods: this cohort study was conducted with a sample of 73 obese individuals at a nutrition and metabolic disease outpatient unit. Anthropometric data were collected, and a three-day food record was used for dietary assessment. BED was identified using the Binge Eating Scale, and patients were divided into two groups (BED and non-BED controls). Patients were reassessed at three-month follow-up. Data were analyzed using Student’s t-tests and generalized estimating equations with Bonferroni correction. Adherence to dietary treatment was assessed by calculating differences between recommended and actual energy intakes. Results: BED was identified in 28.7 % of obese individuals. There was no significant difference in adherence to dietary treatment between BED and control patients. Both groups showed similar eating habits and physical activity levels throughout the study period. Conclusion: obesity is one of the factors contributing to the lack of adherence to nutritional guidelines. The importance of cognitive methods in dietary treatment programs should be further investigated. (AU)

Introducción: el trastorno por atracón compulsivo (TA) es el trastorno alimentario que más afecta a la población obesa, y el TA es un aspecto que posiblemente interfiere en la tasa de adherencia al tratamiento nutricional, que también es baja en individuos con enfermedades crónicas no transmisibles. Objetivo: examinar la relación entre la adherencia al tratamiento dietético y la presencia de TA en individuos obesos.Métodos: este estudio de cohorte se realizó con una muestra de 73 personas obesas en una unidad de nutrición y enfermedades metabólicas ambulatorias. Se recogieron datos antropométricos y se utilizó un registro alimentario de tres días para la evaluación dietética. El trastorno por atracón compulsivo (BED, por sus siglas en inglés) se identificó usando la Binge Eating Scale, y los pacientes se dividieron en dos grupos (BED y controles sin BED). Los pacientes fueron reevaluados a los tres meses de seguimiento. Los datos se analizaron utilizando pruebas t de Student y ecuaciones de estimación generalizadas con corrección de Bonferroni. La adherencia al tratamiento dietético se evaluó calculando las diferencias entre la ingesta energética recomendada y la real. Resultados: se identificó BED en el 28,7 % de los individuos obesos. No hubo diferencias significativas en la adherencia al tratamiento dietético entre los pacientes con BED y los de control. Ambos grupos mostraron hábitos alimentarios y niveles de actividad física similares durante todo el periodo de estudio. Conclusión: la obesidad es uno de los factores que contribuyen a la falta de adherencia a las pautas nutricionales. La importancia de los métodos cognitivos en los programas de tratamiento dietético debe investigarse más a fondo. (AU)

Glucocorticoid Receptor Gene (NR3C1) Polymorphisms and Metabolic Syndrome: Insights from the Mennonite Population.

The regulation of the hypothalamic-pituitary-adrenal (HPA) axis is associated with polymorphisms and the methylation degree of the glucocorticoid receptor gene (NR3C1) and is potentially involved in the development of metabolic syndrome (MetS). In order to evaluate the association between MetS with the polymorphisms, methylation, and gene expression of the NR3C1 in the genetically isolated Brazilian Mennonite population, we genotyped 20 NR3C1 polymorphisms in 74 affected (MetS) and 138 unaffected individuals without affected first-degree relatives (Co), using exome sequencing, as well as five variants from non-exonic regions, in 70 MetS and 166 Co, using mass spectrometry. The methylation levels of 11 1F CpG sites were quantified using pyrosequencing (66 MetS and 141 Co), and the NR3C1 expression was evaluated via RT-qPCR (14 MetS and 25 Co). Age, physical activity, and family environment during childhood were associated with MetS. Susceptibility to MetS, independent of these factors, was associated with homozygosity for rs10482605*C (OR = 4.74, pcorr = 0.024) and the haplotype containing TTCGTTGATT (rs3806855*T_ rs3806854*T_rs10482605*C_rs10482614*G_rs6188*T_rs258813*T_rs33944801*G_rs34176759*A_rs17209258*T_rs6196*T, OR = 4.74, pcorr = 0.048), as well as for the CCT haplotype (rs41423247*C_ rs6877893*C_rs258763*T), OR = 6.02, pcorr = 0.030), but not to the differences in methylation or gene expression. Thus, NR3C1 polymorphisms seem to modulate the susceptibility to MetS in Mennonites, independently of lifestyle and early childhood events, and their role seems to be unrelated to DNA methylation and gene expression.

Investigation into Brazilian Palygorskite for Its Potential Use as Pharmaceutical Excipient: Perspectives and Applications.

Palygorskite is an aluminum and magnesium silicate characterized by its fibrous morphology, providing it with great versatility in industrial applications, including pharmaceuticals. Although most of the reserves are in the United States, in recent years occurrences of commercially exploited deposits in Brazil have been recorded, mainly in the country's northeast region. This has motivated this study, which analyzes raw Brazilian palygorskite compared to a commercial sample (Pharmasorb® colloidal) to demonstrate its pharmaceutical potential. The chemical and mineral composition of the samples were evaluated for surface properties, granulometry, morphology, crystallography, thermal analysis, and spectroscopy. Raw palygorskite presented 67% purity, against 74% for Pharmasorb® colloidal. The percentage purity relates to the presence of contaminants, mainly carbonates and quartz (harmless under conventional conditions of pharmaceutical use). Furthermore, it was possible to confirm the chemical composition of these phyllosilicates, formed primarily of silicon, aluminum, and magnesium oxides. The crystallographic and spectroscopic profiles were consistent in both samples, showing characteristic peaks for palygorskite (2θ = 8.3°) and bands attributed to fibrous phyllosilicates below 1200 cm-1, respectively. The thermal analysis allowed the identification of the main events of palygorskite, with slight differences between the evaluated samples: loss of water adsorbed onto the surface (~85 °C), removal of water contained in the channels (~200 °C), coordinated water loss (~475 °C), and, finally, the dehydroxylation (>620 °C). The physicochemical characteristics of raw palygorskite align with pharmacopeial specifications, exhibiting a high specific surface area (122 m2/g), moderately negative charge (-13.1 mV), and compliance with the required limits for heavy metals and arsenic. These favorable technical attributes indicate promising prospects for its use as a pharmaceutical ingredient in the production of medicines and cosmetics.

Pentoxifylline and tocopherol as prophylaxis for osteonecrosis of the jaw due to bone-modifying agents in patients with cancer submitted to tooth extraction: a case series.

PURPOSE: To assess the prophylaxis effect of pentoxifylline and tocopherol (PENTO) on the frequency and severity of medication-related osteonecrosis of the jaw (MRONJ) diagnosed at three months in patients with cancer submitted to tooth extractions during the treatment with bone-modifying agents. METHODS: This case series was conducted at the outpatient dental clinic of the Instituto de Medicina Integral Prof. Fernando Figueira (IMIP) between April 2021 and April 2022. Patients ≥ 18 years old were included; those with maxillary metastasis or who performed head or neck radiotherapy were excluded. The PENTO protocol was prescribed two weeks before and two weeks after the tooth extraction, and patients were reassessed one week, one month, and three months after the extraction. The main outcome was the development of MRONJ. RESULTS: Of the 114 screened patients, 17 were included; they were aged between 43 and 73 years and were mostly female (88.2%). Thirty-two tooth extractions were performed (22 in the maxilla and 10 in the mandible). Breast cancer was the most predominant neoplasm (70.6%), being metastatic in 35.3% of patients. Also, all patients used intravenous bisphosphonates. Stage 1 MRONJ was diagnosed in three patients (17.6%), representing three (9.4%) of all tooth extractions. The repair of MRONJ was achieved 30 days after the PENTO protocol. CONCLUSION: The prophylaxis use of PENTO reduced the severity of injuries, was well-tolerated, and showed patient compliance.

Relationship between adherence to dietary treatment and binge-eating disorder in obese patients.

Introduction: Introduction: the binge eating disorder (BED) is the eating disorder that most affects the obese population, and BED is an aspect that possibly interferes with the rate of adherence to nutritional treatment, which is also low in individuals with chronic non-communicable disease. Objective: to examine the relationship between adherence to dietary treatment and BED presence in obese individuals. Methods: this cohort study was conducted with a sample of 73 obese individuals at a nutrition and metabolic disease outpatient unit. Anthropometric data were collected, and a three-day food record was used for dietary assessment. BED was identified using the Binge Eating Scale, and patients were divided into two groups (BED and non-BED controls). Patients were reassessed at three-month follow-up. Data were analyzed using Student's t-tests and generalized estimating equations with Bonferroni correction. Adherence to dietary treatment was assessed by calculating differences between recommended and actual energy intakes. Results: BED was identified in 28.7 % of obese individuals. There was no significant difference in adherence to dietary treatment between BED and control patients. Both groups showed similar eating habits and physical activity levels throughout the study period. Conclusion: obesity is one of the factors contributing to the lack of adherence to nutritional guidelines. The importance of cognitive methods in dietary treatment programs should be further investigated.

Introducción: Introducción: el trastorno por atracón compulsivo (TA) es el trastorno alimentario que más afecta a la población obesa, y el TA es un aspecto que posiblemente interfiere en la tasa de adherencia al tratamiento nutricional, que también es baja en individuos con enfermedades crónicas no transmisibles. Objetivo: examinar la relación entre la adherencia al tratamiento dietético y la presencia de TA en individuos obesos. Métodos: este estudio de cohorte se realizó con una muestra de 73 personas obesas en una unidad de nutrición y enfermedades metabólicas ambulatorias. Se recogieron datos antropométricos y se utilizó un registro alimentario de tres días para la evaluación dietética. El trastorno por atracón compulsivo (BED, por sus siglas en inglés) se identificó usando la Binge Eating Scale, y los pacientes se dividieron en dos grupos (BED y controles sin BED). Los pacientes fueron reevaluados a los tres meses de seguimiento. Los datos se analizaron utilizando pruebas t de Student y ecuaciones de estimación generalizadas con corrección de Bonferroni. La adherencia al tratamiento dietético se evaluó calculando las diferencias entre la ingesta energética recomendada y la real. Resultados: se identificó BED en el 28,7 % de los individuos obesos. No hubo diferencias significativas en la adherencia al tratamiento dietético entre los pacientes con BED y los de control. Ambos grupos mostraron hábitos alimentarios y niveles de actividad física similares durante todo el periodo de estudio. Conclusión: la obesidad es uno de los factores que contribuyen a la falta de adherencia a las pautas nutricionales. La importancia de los métodos cognitivos en los programas de tratamiento dietético debe investigarse más a fondo.

Hemodynamic and motion demands of soccer referees: a comparison between series A and B of the State Championship of Rio de Janeiro, Brazil / Las demandas hemodinámicas y de movimiento de los árbitros de fútbol: una comparación entre las series A y B del Campeonato Estatal de Río de Janeiro, Brasil

Introduction: Soccer referees need excellent conditioning to withstand the physical and psychological demands of games.Objective: To compare the hemodynamic variables, speed, cadence, and distance coursed of referees during soccer gamesof series A and B in Rio de Janeiro, Brazil. Material and method: The total number of decisions made during the 10 soccer matches evaluated was 1,224 observabledecisions of 10 professional Soccer referees (one per soccer match: 5 in series A and 5 in series B). We used a frequency meter(Polar, model V800, PolarFlow software) and video footage of the games (Sony, model PXW-Z150, 4K). The moments considered were: the decision, 15 seconds that preceded it, and the period from the beginning of each stage to each decision. Were studied the hemodynamic [average heat rate (mean HR), maximum heart rate (HRmax), and minimum heart rate (HRmin)]and motion variables [average speed (Vmed), maximum speed (Vmax), average cadence (cadencemed), maximum cadence(cadencemax), minimum cadence (cadencemin), and distance covered]. Descriptive measures were used to present the resultsof the variables studied and the Student’s T-Test for independent samples to test the study hypotheses. The significance levelwas set at 95% (P <0.05). Results: The matches of series A had a greater number of interventions and greater hemodynamic load at the exact momentof the decision than those of series B. significantly (P <0.05): mean HR, HRmax, HRmin, Vmax, Cadencemed, and Cadencemax in seriesA were higher compared to series B. In the 15 seconds before the decisions: mean HR, HRmax, and HRmin in series A were higherthan in series B, and Vmed in series B was higher in relation to series A. At the exact moment of the decisions: mean HR in seriesA was higher in relation to series B...(AU)

Introducción: Los árbitros de fútbol necesitan un excelente acondicionamiento para soportar las exigencias físicas y psicológicas de los partidos. Objetivo: Comparar las variables hemodinámicas [frecuencia cardíaca media (mean HR), frecuencia cardíaca máxima (HRmax)y frecuencia cardíaca mínima (HRmin)] y desplazamiento [velocidad media (Vmed), velocidad máxima (Vmax), cadencia media(cadencemed), cadencia máxima (cadencemax), cadencia mínima (cadencemin) y distancia recorrida] durante intervencionesarbitrales en partidos entre las series A y B en Río de Janeiro, Brasil. Material y método: Se analizaron 1.224 decisiones observables de 10 árbitros profesionales de fútbol cada uno en 1 partido(10 partidos del Campeonato Carioca: 5 en la serie A y 5 en la B). Se utilizaron frecuencímetros (Polar, modelo V800, softwarePolarFlow) y secuencias de video de los juegos (Sony, modelo PXW-Z150, 4K). Los momentos considerados fueron: la decisión,los 15 segundos que la precedieron y el tiempo desde el inicio de cada etapa hasta cada decisión. Resultados: Los partidos de la serie A tuvieron mayor número de intervenciones y mayor carga hemodinámica en el momentoexacto de la decisión que los de la serie B. significativamente (p <0,05): mean HR, HRmax, HRmin, Vmax, cadencemed y cadencemaxin la serie A fueron mayores que en la serie B. En los 15 segundos previos a las decisiones: mean HR, HRmax y HRmin en la serieA fueron mayores con relación a la serie B, la Vmed en la serie B fue mayor en relación a la serie A. Conclusión: Las intervenciones de los árbitros generalmente se realizan bajo presión hemodinámica elevada. Los partidosdisputados en la serie A requieren un número de intervenciones e intensidad hemodinámica superior a los partidos de laserie B...(AU)

Deep-Masker: A Deep Learning-based Tool to Assess Chord Length from Murine Lung Images.

Chord length is an indirect measure of alveolar size and a critical endpoint in animal models of chronic obstructive pulmonary disease (COPD). In assessing chord length, the lumens of nonalveolar structures are eliminated from measurement by various methods, including manual masking. However, manual masking is resource intensive and can introduce variability and bias. We created a fully automated deep learning-based tool to mask murine lung images and assess chord length to facilitate mechanistic and therapeutic discovery in COPD called Deep-Masker (available at http://47.93.0.75:8110/login). We trained the deep learning algorithm for Deep-Masker using 1,217 images from 137 mice from 12 strains exposed to room air or cigarette smoke for 6 months. We validated this algorithm against manual masking. Deep-Masker demonstrated high accuracy with an average difference in chord length compared with manual masking of -0.3 ± 1.4% (rs = 0.99) for room-air-exposed mice and 0.7 ± 1.9% (rs = 0.99) for cigarette-smoke-exposed mice. The difference between Deep-Masker and manually masked images for change in chord length because of cigarette smoke exposure was 6.0 ± 9.2% (rs = 0.95). These values exceed published estimates for interobserver variability for manual masking (rs = 0.65) and the accuracy of published algorithms by a significant margin. We validated the performance of Deep-Masker using an independent set of images. Deep-Masker can be an accurate, precise, fully automated method to standardize chord length measurement in murine models of lung disease.