Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 10 de 10
Filter
1.
Front Vet Sci ; 11: 1360593, 2024.
Article in English | MEDLINE | ID: mdl-38756520

ABSTRACT

Dirofilaria immitis infection is routinely detected in dogs during veterinary care in tropical and subtropical regions worldwide. Parasitological tests for the detection of this infection are routinely performed only in areas with a high prevalence. Baixada Fluminense, a region in Rio de Janeiro, was considered heartworm-free until local veterinarians began to receive blood exams results indicating the presence of microfilariae (MF). A laboratory database was hence used to collect data from 2017 to 2020 to understand the extent of spread of the parasite in this area. The results of complete blood count analysis and MF or heartworm antigen detection tests conducted on canine samples sent from veterinary clinics in Baixada Fluminense (Magé, Duque de Caxias, Guapimirim, Nova Iguaçu, and São João de Meriti municipalities) were included. In total, the results of 16,314 hematological tests were considered. The overall prevalence of D. immitis was 3.4% (554/16,314), considering that only one test result was obtained per animal on the same day. This study is highly relevant because it indicates the spreading geographic distribution of the worms, heightens awareness among local health professionals and the general population, and encourages compliance with prophylactic measures to prevent further spread of parasite.

2.
Front Public Health ; 10: 848633, 2022.
Article in English | MEDLINE | ID: mdl-35692307

ABSTRACT

Schistosomiasis is a widely distributed parasitic disease and one of the most important neglected tropical diseases globally, for which Praziquantel® (PZQ) is the only available treatment. In this context, tests with new PZQ formulations become relevant for disease control. This study evaluated the effects of PZQ treatment in the prepatent phase of schistosomiasis using two formulations: nanoencapsulated (PZQ-NANO) and active pharmaceutical ingredient (PZQ-API). Five experimental groups were established, for which the following serological parameters were evaluated: ALT, AST, ALP, and TP. Animals treated with PZQ-API at 15 and 30 days post-infection showed decreased eggs per gram of feces (EPG) compared to untreated infected animals. The same animals showed reductions of 63.6 and 65.1%, respectively, at 60 days post-infection. Animals treated with PZQ-NANO experienced no significant changes in EPG at any time of observation. Animals treated with either PZQ-API or PZQ-NANO had higher ALT and AST levels in the patent period (60 and 90 days post-infection). Treatment with PZQ, either API or NANO, at 15 days post-infection reduced AST, ALT, and TP levels. It is concluded that prepatent treatment with PZQ-API can reduce the parasite load of infected animals and that treatment at 15 days post-infection can prevent increased serum levels of ALT, AST, and TP.


Subject(s)
Schistosomiasis mansoni , Schistosomiasis , Animals , Disease Models, Animal , Mice , Praziquantel/pharmacology , Praziquantel/therapeutic use , Schistosoma mansoni , Schistosomiasis/drug therapy , Schistosomiasis/prevention & control , Schistosomiasis mansoni/parasitology
3.
Parasitol Res ; 121(3): 925-932, 2022 Mar.
Article in English | MEDLINE | ID: mdl-35083588

ABSTRACT

Dirofilaria immitis is the causative agent of canine heartworm disease, a severe health problem in dogs, especially in coastal areas of tropical and subtropical regions of the world. We employed molecular methods to investigate the occurrence of canine infection by filarioids in five municipalities of Baixada Fluminense (Magé, Duque de Caxias, Guapimirim, Nova Iguaçu, and São João de Meriti), a non-endemic area of Rio de Janeiro State, Southeast Brazil. A total of 110 canine blood samples collected from 2017 to 2018 and positive for microfilariae at the modified Knott's test were screened by cPCR targeting DNA fragments of the 12S rDNA gene for filarial nematodes. Seventy-seven samples (70%) tested positive at the molecular analysis. Of these, 72 were identified as D. immitis and 5 (4.5%) as Acanthocheilonema reconditum. Dirofilaria repens was not detected in the studied municipalities of Baixada Fluminense. This is the first record of D. immitis and A. reconditum in the Baixada Fluminense region, Rio de Janeiro State, Brazil. The prevalence of D. immitis cases in the five municipalities suggests the establishment and maintenance of its enzootic cycle in the studied region, which indicate vulnerability to the occurrence of epidemic cycles and, possibly, human cases.


Subject(s)
Dirofilaria immitis , Dirofilaria repens , Dirofilariasis , Dog Diseases , Animals , Brazil/epidemiology , Dirofilaria immitis/genetics , Dirofilaria repens/genetics , Dirofilariasis/epidemiology , Dog Diseases/epidemiology , Dogs , Prevalence
4.
World J Hepatol ; 10(11): 867-876, 2018 Nov 27.
Article in English | MEDLINE | ID: mdl-30533187

ABSTRACT

AIM: To evaluate viral hepatitis knowledge among individuals from different resource areas and health conditions to identify possible gaps. METHODS: A cross-sectional, descriptive study was carried out among 447 individuals from five distinct populations in Brazil: Southeast Viral Hepatitis Ambulatory (n = 100), South (n = 89) and Northeast (n = 114) Health Center, Southeast (n = 77) and Northeast (n = 67) low resource areas. All individuals answered a questionnaire assessing sociodemographic characteristics and viral hepatitis awareness. The perception was scored based on the average number of correct answers of all participants and categorized as "low" (0-28 correct answers) or "desirable" (29-46 correct answers). Associations between sociodemographic characteristics and perception were also evaluated. RESULTS: A low level of knowledge was observed in individuals from Northeast Health Center, Northeast and Southeast low resource areas while desirable knowledge was observed in individuals from Viral Hepatitis Ambulatory and South Health Center. According to sociodemographic characteristics, desirable scores were more common among those with secondary education (47.1%), those who declared themselves as white (46.3%), and those who lived in houses with three individuals (25.5%). Multivariate analysis showed an association between viral hepatitis perception and type of population. CONCLUSION: The results demonstrated high level of knowledge among study participants from health clinics from the Southeast region of Brazil and the importance of education programs in increasing the level of knowledge in low resource areas.

5.
J Trop Med ; 2018: 8159354, 2018.
Article in English | MEDLINE | ID: mdl-30105057

ABSTRACT

INTRODUCTION: The drought in the Brazilian semiarid region has affected the quality of water. This study assessed the relationships between enteric parasitoses, water management, and water quality, correlating them with pluviometric seasonality. METHODS: Cross-sectional surveys were carried out in four rural communities at the beginning of the dry season (n=151), at the end of the dry season (n=184), and in the rainy season (n=199), in order to collect sociodemographic data, human fecal samples, and samples of the water used for human consumption for physicochemical and microbiological analyses. In 2015, water filters were provided to 30 households under study. RESULTS: There was an increasing trend in detection rates of commensal protozoa and the Entamoeba histolytica/Entamoeba dispar complex at the beginning of the rainy season, with detection rates of 6% in 2014 and 21.6% in 2016. Giardia intestinalis and Ascaris lumbricoides presented distinct temporal distributions, which peaked in 2015: 20.1% and 30%, respectively. The proportion of inhabitants drinking inadequate water was 55% at the beginning of the dry season and 28.8% at the end of the dry season, reaching 70.9% at the beginning of the rainy season. The presence of filters reduced this proportion among those who received the hollow ceramic candle filter. CONCLUSIONS: Data suggest that the strategies to increase water supply in the Brazilian semiarid region can be ameliorated in order to improve the quality of drinking water.

6.
Prim Health Care Res Dev ; 19(4): 333-343, 2018 07.
Article in English | MEDLINE | ID: mdl-29113608

ABSTRACT

AimThis study analyzed the management of intestinal parasitic infections in the Family Health Strategy covering Brazilian urban slums. BACKGROUND: The Family Health Strategy is the preferred strategy for providing public, community-based primary health care in the Brazilian Unified Health System (SUS). Through this strategy, Family Health teams are responsible for the health of residents of a defined territory, including health promotion, health education and control of neglected tropical diseases such as intestinal parasitic infections. METHODS: Knowledge, attitudes and practices surveys were applied with Family Health team members (n=58) and patients (n=571) of an agglomeration of Brazilian urban slums in Rio de Janeiro.FindingsThe management of intestinal parasitic infections and health promotion were limited. Health education was not considered an essential aspect of team members' work and did not include environmental or social determinants of health. Community health workers and urban slum residents presented similar knowledge, attitudes and practices regarding intestinal parasitic infections. CONCLUSIONS: Multiple, competing demands promote prioritization of the aspects of care where curative, biomedical activities predominate over prevention and an integral approach to health. However, the complex processes involving the cycle of poverty and disease go beyond the biomedical, limiting the potential for health in urban slums. Implications include a need to better prepare health professionals for primary health care services through reflection on local concerns and the social determinants of health, highlighting the importance of territorialized care and permanent education.


Subject(s)
Family Health/education , Health Knowledge, Attitudes, Practice , Health Personnel/education , Health Personnel/psychology , Health Promotion/methods , Intestinal Diseases, Parasitic/therapy , Patient Education as Topic/methods , Adult , Aged , Aged, 80 and over , Brazil , Female , Humans , Male , Middle Aged , Poverty Areas , Urban Population
7.
Acta Trop ; 130: 51-7, 2014 Feb.
Article in English | MEDLINE | ID: mdl-24161877

ABSTRACT

This original study suggests that alterations observed on tegumental structure and egg quality of adult Schistosoma mansoni harvested from TS mice are due to their high immune tolerogenic and low-inflammatory capacity. The tegument of worms harvested from genetically selected mice for extreme phenotypes of immune oral tolerance, resistance (TR) and susceptibility (TS) were analyzed by transmission electron microscopy (TEM). Parasites recovered from TR mice showed no tegumental morphological changes. However, specimens collected from TS mice exhibited tubercle swelling with blunted and shortened spines in lower density. These tegumental alterations were similar to those described with artemether or praziquantel treatment, but without to affecting the worm surveillance, supporting observations that the host immune system influences the development and function of the tegument of worms harbored in non-antihelminthic treated TS mice. TS mice showed a higher percentage of dead eggs and a lower percentage of immature eggs than TR mice, but had similar quantities of collected eggs. This suggests that in TS mice the alterations in adult worm tegument prevented egg development, but not egg production or worm survival. These results corroborate our previous scanning electron microscopy (SEM) study indicating the influence of the host immune regulatory profile on the development and function of the worm's reproductive system and tegument.


Subject(s)
Schistosoma mansoni/ultrastructure , Animals , Artemether , Artemisinins/pharmacology , Disease Susceptibility , Female , Male , Mice , Mice, Inbred Strains , Microscopy, Electron, Transmission , Praziquantel/pharmacology , Schistosoma mansoni/drug effects , Schistosomiasis mansoni/immunology , Schistosomiasis mansoni/parasitology
8.
Acta Trop ; 116(1): 95-9, 2010 Oct.
Article in English | MEDLINE | ID: mdl-20542008

ABSTRACT

The present study exhibits original results of S. mansoni tegumental alterations due to contact with the immune system of non anti-helminthic treated mice. We compared, by SEM, the tegument of adult worms recovered from strains of mice genetically selected to extreme phenotypes of resistance (TR strain) and susceptibility (TS strain) to egg-albumin oral tolerance (OT). The parasites recovered from TR mice displayed no morphologic alteration, while specimens collected from TS mice presented tubercle swelling with blunted and shortened spines in lower density, increased sensory organelle numbers, fusion and tegumental ridge peeling. These tegument alterations were similar to those described for Artemether or Praziquantel treatment, supporting observations that the host immune system influences the development and function of the tegument of worms harbored in both anti-helminthic treated and non-treated mice. Our results are indicative that the development and function of the worm tegument depend on the immune regulatory capacity of each individual host.


Subject(s)
Schistosoma mansoni/ultrastructure , Schistosomiasis mansoni/immunology , Animals , Disease Models, Animal , Female , Immune Tolerance , Immunity, Active , Male , Mice , Mice, Inbred Strains , Microscopy, Electron, Scanning , Schistosoma mansoni/immunology
9.
Vet Parasitol ; 151(2-4): 227-32, 2008 Feb 14.
Article in English | MEDLINE | ID: mdl-18155840

ABSTRACT

The genus Libyostrongylus includes three species, L. douglassii, L. dentatus and L. magnus that occur as parasites in the proventriculus of Struthio camelus. We confirmed a mixed infection by L. douglassii and L. dentatus in farmed ostriches from the southeast of Brazil for the first time, and provided new information on some morphological characters that differentiate these species. Adult nematodes collected from the proventriculus of ostriches were observed by light and scanning electron microscopy. Morphologic characterization and morphometric analysis of the nematodes enabled the distinction of both species and corroborated results of prior studies. Specimens of L. dentatus have a buccal capsule with a prominent esophageal tooth. Furthermore, males and females of L. dentatus were larger (4954 and 9347 microm) than those of L. douglassii (3411 and 4229 microm), but measurements for most characters in both species were smaller then those previously reported. Besides, the cephalic structure based on scanning electron microscopy differs, and L. dentatus has thick lips with round papillae, whereas L. douglassii has fine lips with lengthened papillae. The confirmation of both species in South America strongly suggests that the mixed infection may be common in farmed ostriches.


Subject(s)
Bird Diseases/parasitology , Struthioniformes/parasitology , Trichostrongyloidea/ultrastructure , Trichostrongyloidiasis/veterinary , Animals , Brazil , Female , Male , Microscopy, Electron, Scanning , Proventriculus/parasitology , Trichostrongyloidea/isolation & purification , Trichostrongyloidiasis/parasitology
10.
Vet Parasitol ; 137(1-2): 175-9, 2006 Apr 15.
Article in English | MEDLINE | ID: mdl-16448757

ABSTRACT

Domestic production of ostrich in Brazil started in the beginning of the last decade, but its sanitary state has not been reported. Libyostrongylus sp. is an ostrich specific nematode whose parasitism can severely affect the birds. Thus, Libyostrongylus spp. larvae were identified in commercial ostriches in the north region of the state of Rio de Janeiro, Brazil. The EPG was determined and fecal cultivation was performed. The eggs presented typical characteristics of strongylid and were present in five out of six farms. The mean EPG varied from 22 to 2395 and Libyostrongylus spp. prevalence was from 0 to 100%, with adult birds more infected. Two types of infective larvae with tail finishing in a tipped spiny knob were distinguished. The first had a mean length of 848 microm (710-1010) with a long sheath tail of about 66 microm (52-112). The other had a mean length of 826 microm (620-940) with a short, more abruptly ending sheath tail of 32 microm (22-40) and a less rounded cephalic end. The differences between these larvae suggest two Libyostrongylus species.


Subject(s)
Bird Diseases/epidemiology , Struthioniformes/parasitology , Trichostrongyloidea/isolation & purification , Trichostrongyloidiasis/veterinary , Animals , Brazil/epidemiology , Feces/parasitology , Parasite Egg Count/veterinary , Phylogeny , Prevalence , Species Specificity , Trichostrongyloidea/anatomy & histology , Trichostrongyloidea/classification , Trichostrongyloidiasis/epidemiology
SELECTION OF CITATIONS
SEARCH DETAIL
...