ABSTRACT
In total, 10 populations of Helicotylenchus associated with Musa spp., six from Colombia, and four from Brazil were identified to the species level. Morphological and morphometric data were recorded for each population, performed a principal components analysis (PCA), a conglomerate, along with consensus sequences of D2 to D3 expansion segment of the large subunit of ribosomal DNA (28S) for different populations. Identify of species were performed using the basic local alignment search tool (BLAST), and the evolutionary relationships of species were explored using a phylogeny study. Four species of Helicotylenchus were identified based on morphological and morphometric data: H. multicinctus, H. dihystera, H. erythrinae, and H. californicus. PCA and conglomerate analysis clearly separated these species. BLAST and phylogenetic analysis confirmed the presence of these species associated with Musa spp. crops. This is the first report of these species in Colombia through integrative taxonomy.In total, 10 populations of Helicotylenchus associated with Musa spp., six from Colombia, and four from Brazil were identified to the species level. Morphological and morphometric data were recorded for each population, performed a principal components analysis (PCA), a conglomerate, along with consensus sequences of D2 to D3 expansion segment of the large subunit of ribosomal DNA (28S) for different populations. Identify of species were performed using the basic local alignment search tool (BLAST), and the evolutionary relationships of species were explored using a phylogeny study. Four species of Helicotylenchus were identified based on morphological and morphometric data: H. multicinctus, H. dihystera, H. erythrinae, and H. californicus. PCA and conglomerate analysis clearly separated these species. BLAST and phylogenetic analysis confirmed the presence of these species associated with Musa spp. crops. This is the first report of these species in Colombia through integrative taxonomy.
ABSTRACT
Three populations, two from Colombia and one from Brazil, of Rotylenchulus reniformis associated with banana and plantain, were characterized using morphological, morphometric, and molecular methods. Morphometric data from these populations were similar to type and reference populations of R. reniformis. Partial sequences of both D2-D3 rDNA and mitochondrial cytochrome oxidase subunit I (COI) regions had a strong affinity (99% similarity) to previously published sequences of R. reniformis. Phylogenetic analyses (maximum likelihood and Bayesian inference) suggested that the Colombian populations of R. reniformis corresponded to the previously described Type A of the species. This is the definitive first report in Colombia of R. reniformis associated with banana and plantain crops.
ABSTRACT
The species Metarhabditis blumi was diagnosed for the first time in Brazil using a molecular assay. Parasites isolated from two cattle herds from the state of Minas Gerais, southeastern Brazil, were classified as M. blumi according to their D2/D3 expansion fragments of the 28S rDNA sequence, which had a high degree of homology (100% similarity) with a M. blumi sequence deposited in GenBank (EU195965.1). The phylogenetic tree comparison also confirmed grouping of the populations evaluated in the current study as M. blumi.
