ABSTRACT
This study proposes the was to evaluate the stability and methane production with organic load differents in an upflow anaerobic sludge blanket reactor (UASB) treating swine wastewater by methods of multivariate analysis. Four organic loads were used with average hydraulic holding times of one day. The methods of data analysis of linear regression, Pearson correlation, principal component analysis and hierarchical clustering analysis were used for understanding stability and methane production in the reactor. The highest concentrations of bicarbonate alkalinity of 683 mg L-1 CaCO3 and total volatile acids of 1418 mg L-1 HAc with maximum organic loading applied were obtained. The optimal stability conditions occurred at an intermediate and partial alkalinity ratio between 0.24 and 0.25 observed in initial phases with a chemical oxygen demand (COD) removal of 47-57%. Maximum methane production was 9.0 L CH4 d-1 observed with linear regression positive and occurred at the highest applied organic load, corresponding to the highest COD removal efficiency and increased microbial biomass. Positive and negative correlation between functional stability in anaerobic digestion showed regular activity between acids, alkalinity and organic matter removal. This fact was also proven by the analysis of principal components that showed three components responsible for explaining 83.2% of the data variability, and the alkalinity, organic matter influent and organic acids had the greatest effects on the stability of the UASB reactor. Hierarchical clusters detected the formation of five groupings with a similarity of 50.1%, indicating that temperature and pH were variables with unitary influences on data dimensionality.
ABSTRACT
The purpose of this study was to analyze the effects of the application of multivariate criteria of principal components and hierarchical clustering as a mechanism for monitoring the performance of Tifton 85 grass (Cynodon spp.) planted in horizontal subsurface flow constructed wetland reactor (HSSF-CW) under different organic (OLR), nutritional and sodium loads of swine wastewater (SW). The HSSF-CW planted with Tifton 85 grass was used as a swine wastewater after treatment applying organic loading rates between 26.1 (1st cut) and 360.6 kg ha-1 day-1 COD (8th cut). The maximum performances of HSSF-CW consisted of 52.0 t ha-1 of productivity and 24.0% of crude protein, with the application of 59.7, 64.2, and 31.2 kg ha-1 day-1 of TKN, PT, and K+, respectively. The eleven original variables generated four new components, with PC4 accounting for 94.0% of total variance, a condition strengthened with four data groupings greater than 48% similarity and three data groupings greater than 95% similarity between the variables. There was a strong association between of nitrogen, phosphorus, and potassium concentration by the hierarchical grouping and the intermediate cuts and lower temperatures.
