Management strategy influences coral oxidative stress responses in a marine protected area in the Southwestern Atlantic.

Coral reefs are experiencing accelerated degradation due to global and local stressors. The understanding of how corals cope with these disturbances is urgent. We focused on elucidating antioxidant capacity responses of the Mussismilia harttii and Siderastrea sp. corals, in reefs with use management in a marine protected area. We tested whether the activity of antioxidant enzymes in healthy colonies is higher at multiple-use reefs than at no-take reef, and whether the activity of antioxidant enzymes is higher for bleached than for healthy Siderastrea sp. colonies. Lipid peroxidation and enzymatic activity found in bleached colonies evidence chronic stress and cellular damage not related to thermal anomalies. Chronic stress in healthy colonies was also found but responses differed among species, being higher at multiple use reefs, mainly for Siderastrea sp. We highlight the role of the local conservation actions in the integrity of coral physiology and reef resilience under global climate changes.

Cylindrospermopsin exposure promotes redox unbalance and tissue damage in the liver of Poecilia reticulata, a neotropical fish species.

There is a growing concern regarding the adverse risks exposure to cylindrospermopsin (CYN) might exert on animals and humans. However, data regarding the toxicity of this cyanotoxin to neotropical fish species are scarce. Using the fish species Poecilia reticulata, the influence of CYN concentrations equal to and above the tolerable for drinking water may produce on liver was determined by assessing biomarkers of antioxidant defense mechanisms and correlated to qualitative and semiquantitative histopathological observations. Adult females were exposed to 0.0 (Control); 0.5, 1 and 1.5 µg/L pure CYN for 24 or 96 hr, in triplicate. Subsequently the livers were extracted for biochemical assays and histopathological evaluation. Catalase (CAT) activity was significantly increased only by 1.5 µg/L CYN-treatment, at both exposure times. Glutathione -S-transferase (GST) activity presented a biphasic response for both exposure times. It was markedly decreased after exposure by 0.5 µg/L CYN treatment but significantly elevated by 1.5 µg/L CYN treatment. All CYN treatments produced histopathological alterations, as evidenced by hepatocyte cords degeneration, steatosis, inflammatory infiltration, melanomacrophage centers, vessel congestion, and areas with necrosis. Further, an IORG >35 was achieved for all treatments, indicative of the presence of severe histological alterations in P. reticulata hepatic parenchyma and stroma. Taken together, data demonstrated evidence that CYN-induced hepatotoxicity in P. reticulata appears to be associated with an imbalance of antioxidant defense mechanisms accompanied by histopathological liver alterations. It is worthy to note that exposure to low environmentally-relevant CYN concentrations might constitute a significant risk to health of aquatic organisms.

Gestational Diabetes Mellitus: The Crosslink among Inflammation, Nitroxidative Stress, Intestinal Microbiota and Alternative Therapies.

Gestational diabetes mellitus (GDM) is characterized by a set of metabolic complications arising from adaptive failures to the pregnancy period. Estimates point to a prevalence of 3 to 15% of pregnancies. Its etiology includes intrinsic and extrinsic aspects of the progenitress, which may contribute to the pathophysiogenesis of GDM. Recently, researchers have identified that inflammation, oxidative stress, and the gut microbiota participate in the development of the disease, with potentially harmful effects on the health of the maternal-fetal binomial, in the short and long terms. In this context, alternative therapies were investigated from two perspectives: the modulation of the intestinal microbiota, with probiotics and prebiotics, and the use of natural products with antioxidant and anti-inflammatory properties, which may mitigate the endogenous processes of the GDM, favoring the health of the mother and her offspring, and in a future perspective, alleviating this critical public health problem.

Euterpe oleracea (Martius) Oil Reverses Testicular Alterations Caused after Cadmium Administration.

Cadmium (Cd) is an environmental pollutant that induces reproductive toxicity by generating reactive oxygen species, which leads to oxidative stress. Euterpe oleracea fruits are known for being rich in oils containing triacylglycerol and phenolic compounds. They are considered as potent antioxidants to be used to counteract Cd effects within the testis. In the present study, adult males Swiss mice were treated with CdCl2 aqueous solution (4.28 mg/kg) by gavage for 7 days. The experimental groups were treated with Euterpe oleracea oil at the doses of 50, 100, and 150 mg/kg, for 42 days. The results showed that Cd intoxication led to increased tubular pathologies, such as reduction in epithelium height and area thus increasing both luminal diameter and tubule-epithelium ratio. Besides, Leydig cell's morphometry indicated reduction in nucleus and cytoplasm volumes of this cell type, which were recovered after E. oleracea oil intake. In addition, serum testosterone levels, testicular Mn and Zn concentrations, SOD and CAT activity, and germ cell viability increased after oil intake. Therefore, E. oleracea oil showed a regenerative effect in the testicular parenchyma negatively affected by Cd, mainly in the animals that received the highest oil concentration (150 mg/kg).

Hydroalcoholic extract of Pfaffia glomerata alters the organization of the seminiferous tubules by modulating the oxidative state and the microstructural reorganization of the mice testes.

ETHNOPHARMACOLOGICAL USAGES: Leaves and roots of Pfaffia glomerata areused as aphrodisiacs, tranquilizers and antirheumatics. Due to the lack of experimental scientific data, studies are necessary to identify its medicinal properties. AIMS: The present study aimed to evaluate the effect of hydroalcoholic root extract of P. glomerata (Brazilian ginseng extract - BGE) on testicular parenchyma, and evaluate possible harmful effects through testicular oxidative stress analysis. MATERIALS AND METHODS: Adult mice were divided into 6 groups: control (water), sildenafil citrate, BGE (100, 200 and 400 mg/kg/day), and BGE (200 mg/kg every three days). RESULTS: The treatment reduced the volumetric proportions of seminiferous tubules and epithelium, the number of Sertoli cells, and increased hydrogen peroxide levels, without affecting sperm production. It also caused cell death and changes in the frequency of stages of the seminiferous epithelium cycles. The 100 mg/kg dose responds in a similar way to sildenafil citrate, promoting changes in the gonadal structure, but with efficient response to contain the damage. CONCLUSIONS: Doses of 200 mg/kg, continuous or discontinuous, induced an increase in testicular nitric oxide, as well as sildenafil citrate, showing be efficient as aphrodisiac, but promotes cell death regardless of the form of administration.

Curcuma longa as additive in the diet for Astyanax aff. bimaculatus.

The aim of the present study was to evaluate the effect of turmeric (Curcuma longa) as additive in the diet for Astyanax aff. bimaculatus. Fish (0.83 ± 0.04 g) were fed, for 60 days, with six diets containing 0.0, 20.0, 40.0, 60.0, 80.0, and 100.0 g turmeric kg-1 feed. There was an increasing linear effect of turmeric on the thickness of the muscular layer, and height and width of the folds of the intestine. In the liver, a quadratic effect was observed of turmeric on the percentage of hepatocyte cytoplasm and a decreasing linear effect on the percentage of sinusoid capillaries. A quadratic effect was also observed of turmeric on the liver glycogen. There was no effect of turmeric on the antioxidant activity in the liver, carcass composition or productive performance of the fish. Thus, we concluded that Curcuma longa has trophic effects on the epithelium and the muscular layer of the intestine of A. aff. bimaculatus. Additionally, low levels of Curcuma longa cause increased deposition of liver glycogen and high levels cause reduction.