ABSTRACT
Phytochemical investigation of Chromolaena laevigata led to the isolation of a new cadinene-sesquiterpene, chromolaevigone glucoside (1), along with nine known compounds: daucosterol (2), stigmasterol glycoside (3), stigmasterol (4), ß-sitosterol (5), pilloin (6), gonzalitosin I (7), quercetin-3-O-α-rhamnopyranoside (8), 7,7-dihydroxy-calamen-12-oic acid lactone (9) and trachelanthic acid (10). Others 11 known compounds were identified by UHPLC-HRMS/MS. These compounds are being described for the first time in this species, with the exception of cadinene 9. Furthermore, due to the limitation of pharmacological studies, antiproliferative, antiviral, and antimicrobial activities of C. laevigata were evaluated. The best results in the cytotoxicity, antimicrobial and antiproliferative tests, presenting GI50 values on ovarian tumour cells (OVCAR-03) of 1.9 µg mL-1 and kidney (786-0) of 2.5 µg mL-1 were observed for the hexanic fraction.[Figure: see text].
Subject(s)
Asteraceae , Chromolaena , Sesquiterpenes , Phytochemicals/pharmacology , Plant Components, AerialABSTRACT
Crude extracts of the marine sponge Chelonaplysilla sp. collected in Samoa, that were obtained from the NCI Open Repository (NCS 21903), inhibited Mycobacterium tuberculosis growth. Assay-guided fractionation of the extract led to the isolation and structural elucidation of the known diterpenoid macfarlandin D (1) and three new diterpenoids macfarlandins F (2), G (3), and H (4). Macfarlandin D (1) exhibited potent antimicrobial activity against M. tuberculosis with an MIC of 1.2 ± 0.4 µg mL-1. Macfarlandins F (2), G (3), and H (4) exhibited significantly weaker antitubercular activities, revealing SAR for the macfarlandin antitubercular pharmacophore. The structures of compounds 2, 3, and 4 were elucidated via detailed analysis of NMR and MS data.
Subject(s)
Diterpenes/chemistry , Diterpenes/pharmacology , Mycobacterium tuberculosis/drug effects , Porifera/chemistry , Animals , Antitubercular Agents/chemistry , Antitubercular Agents/pharmacology , Magnetic Resonance Spectroscopy/methods , Microbial Sensitivity Tests/methodsABSTRACT
Streptomyces sp. 4054, isolated from marine sediments, produced a new butenolide, mycenolide A (1), along with five known butenolide derivatives (2-6). The structures of the compounds were established based on 1D- and 2D-NMR spectroscopic analysis, circular dichroism, and mass spectrometry data. The antimicrobial activity of the crude extract of the marine bacteria Streptomyces sp. 4054 was evaluated, showing good results against Bacillus subtilis and MRSA.
