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1.
Arch Toxicol ; 95(5): 1779-1791, 2021 05.
Article in English | MEDLINE | ID: mdl-33674969

ABSTRACT

Crack cocaine users are simultaneously exposed to volatilized cocaine and to its main pyrolysis product, anhydroecgonine methyl ester (AEME). Although the neurotoxic effects of cocaine have been extensively studied, little is known about AEME or its combination. We investigated cell death processes using rat primary hippocampal cells exposed to cocaine (2 mM), AEME (1 mM) and their combination (C + A), after 1, 3, 6 and 12 h. Cocaine increased LC3 I after 6 h and LC3 II after 12 h, but reduced the percentage of cells with acid vesicles, suggesting failure in the autophagic flux, which activated the extrinsic apoptotic pathway after 12 h. AEME neurotoxicity did not involve the autophagic process; rather, it activated caspase-9 after 6 h and caspase-8 after 12 h leading to a high percentage of cells in early apoptosis. C + A progressively reduced the percentage of undamaged cells, starting after 3 h; it activated both apoptotic pathways after 6 h, and was more neurotoxic than cocaine and AEME alone. Also, C + A increased the phosphorylation of p62 after 12 h, but there was little difference in LC3 I or II, and a small percentage of cells with acid vesicles at all time points investigated. In summary, the present study provides new evidence for the neurotoxic mechanism and timing response of each substance alone and in combination, indicating that AEME is more than just a biological marker for crack cocaine consumption, as it may intensify and hasten cocaine neurotoxicity.


Subject(s)
Cocaine/analogs & derivatives , Animals , Cocaine/toxicity , Gas Chromatography-Mass Spectrometry , Hippocampus , Neurons , Neurotoxicity Syndromes , Pyrolysis , Rats
2.
Ecotoxicol Environ Saf ; 170: 314-323, 2019 Apr 15.
Article in English | MEDLINE | ID: mdl-30530184

ABSTRACT

Life expectancy is increasing worldwide. Lung aging is a process marked by changes in multiple morphological, physiological and age-related biomarkers (e.g., sirtuins) and is influenced by external factors, such as air pollution. Hence, the elderly are considered more vulnerable to the air pollution hazards. We hypothesized that diesel exhaust (DE) exposure intensifies changes in lung inflammatory and structural parameters in aging subjects. Two- and fifteen-month-old mice were exposed to DE for 30 days. Lung function was measured using the forced oscillation method. The inflammatory profile was evaluated in the bronchoalveolar lavage fluid (BALF) and blood, and lung volumes were estimated by stereology. Antioxidant enzyme activity was evaluated by spectrophotometry, sirtuin 1 (SIRT1), sirtuin 2 (SIRT2) and sirtuin 6 (SIRT6) expression was assessed by reverse transcription polymerase chain reaction (RT-PCR), and levels of the sirtuin proteins were evaluated by immunohistochemical staining in lung tissues. Older mice presented decreased pulmonary resistance and elastance, increased macrophage infiltration and decreased tumor necrosis factor (TNF) and interleukin 10 (IL-10) levels in the BALF, reduced activities of the antioxidant enzymes glutathione peroxidase (GPx) and glutathione reductase (GR), and increased activity glutathione S-transferase (GST); increased lung volumes with decreased elastic fiber and increased airway collagen content. SIRT1 gene expression was decreased in older animals, but protein levels were increased. DE exposure increased macrophage infiltration and oxidative stress in the lungs of animals of both ages. SIRT6 gene expression was decreased by DE exposure, with increased protein levels. In older animals, DE affected lung structure and collagen content. Lung aging features, such as decreased antioxidant reserves, lower IL-10 expression, and decreased SIRT1 levels may predispose subjects to exacerbated responses after DE exposure. Our data support the hypothesis that strategies designed to reduce ambient air pollution are an important step towards healthy aging.


Subject(s)
Aging/drug effects , Air Pollutants/toxicity , Lung/drug effects , Particulate Matter/toxicity , Pneumonia/chemically induced , Vehicle Emissions/toxicity , Aging/immunology , Aging/pathology , Air Pollutants/analysis , Animals , Antioxidants/metabolism , Biomarkers/blood , Biomarkers/metabolism , Bronchoalveolar Lavage Fluid/immunology , Lung/immunology , Lung/pathology , Male , Mice , Oxidative Stress/drug effects , Particulate Matter/analysis , Pneumonia/immunology , Pneumonia/pathology , Respiratory Function Tests , Sirtuins/genetics , Vehicle Emissions/analysis
3.
Mediators Inflamm ; 2016: 9303126, 2016.
Article in English | MEDLINE | ID: mdl-27293324

ABSTRACT

Formaldehyde is ubiquitous pollutant that induces oxidative stress in the lung. Several lung diseases have been associated with oxidative stress and their control is necessary. Photobiomodulation therapy (PBMT) has been highlighted as a promissory treatment, but its mechanisms need to be better investigated. Our objective was to evaluate the effects of PBMT on the oxidative stress generated by FA exposure. Male Wistar rats were submitted to FA exposure of 1% or vehicle (3 days) and treated or not with PBMT (1 and 5 h after each FA exposure). Rats treated only with laser were used as control. Twenty-four hours after the last FA exposure, we analyzed the effects of PBMT on the generation of nitrites and hydrogen peroxide, oxidative burst, glutathione reductase, peroxidase, S-transferase enzyme activities, the gene expression of nitric oxide, cyclooxygenase, superoxide dismutase, the catalase enzyme, and heme oxygenase-1. PBMT reduced the generation of nitrites and hydrogen peroxide and increased oxidative burst in the lung cells. A decreased level of oxidant enzymes was observed which were concomitantly related to an increased level of antioxidants. This study provides new information about the antioxidant mechanisms of PBMT in the lung and might constitute an important tool for lung disease treatment.


Subject(s)
Formaldehyde/toxicity , Low-Level Light Therapy , Lung/drug effects , Lung/metabolism , Animals , Antioxidants/metabolism , Catalase/metabolism , Glutathione Reductase/metabolism , Heme Oxygenase-1/metabolism , Hydrogen Peroxide/metabolism , Lung/immunology , Male , Nitric Oxide/metabolism , Nitrites/metabolism , Oxidative Stress/drug effects , Peroxidase/metabolism , Prostaglandin-Endoperoxide Synthases/metabolism , Rats , Rats, Wistar , Respiratory Burst/drug effects , Superoxide Dismutase/metabolism
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