ABSTRACT
Dengue is the most prevalent arthropod-borne viral illness in humans worldwide. Single-nucleotide polymorphisms (SNPs) in genes involved in the immune response, such as dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN), IgG Fc receptor II-A (FcγRIIa), vitamin D receptor (VDR), and tumor necrosis factor alpha (TNF-α), were previously reported to be associated with susceptibility to dengue disease in different human populations. Therefore, due to the relevant association of host immune and genetic status with disease susceptibility/severity of dengue, this work aims to verify the frequency of anti-dengue virus antibodies and some dengue-associated risk SNPs in a population in Minas Gerais State, Southeast Brazil. A total of 1560 individuals were genotyped for polymorphisms in DC-SIGN (rs4804803), FcγRIIa (rs1801274), VDR (rs7975232), and TNF-α (rs1800629). The presence of anti-dengue antibodies (IgM and/or IgG) in these samples was also assayed. Anti-dengue antibodies were detected at an overall frequency of 16.86%, indicating a virus infection in asymptomatic individuals. The genotypic frequencies of all SNPs studied did not differ between the asymptomatic and control groups. Regarding the allelic frequencies of the four SNPs analyzed, a higher frequency was detected of the G allele of FcγRIIa/rs1801274 in the asymptomatic individuals when compared to that in the control group (p = 0.03). Therefore, the results showed a high prevalence of asymptomatic individuals in Minas Gerais State, with a potential association between the presence of the G allele of FcγRIIa/rs1801274 and protection against symptomatic disease.
Subject(s)
Dengue/genetics , Dengue/immunology , Receptors, IgG/genetics , Adult , Arginine/genetics , Brazil , Cell Adhesion Molecules/genetics , Female , Gene Frequency , Genetic Association Studies , Genetic Predisposition to Disease , Humans , Lectins, C-Type/genetics , Male , Middle Aged , Polymorphism, Single Nucleotide , Prevalence , Receptors, Calcitriol/genetics , Receptors, Cell Surface/genetics , Tumor Necrosis Factor-alpha/geneticsABSTRACT
Resumo Objetivo: Avaliar a variação temporal e a correlação entre o bem-estar espiritual e coping religioso/espiritual de pessoas com insuficiência renal crônica em tratamento hemodialítico. Metodologia: Estudo descritivo, analítico e longitudinal. A amostra inicial contou com 118 participantes com perda de 31 ao final das coletas, o que reduziu a amostra final para 87 participantes. Foram aplicados, por meio de entrevista, o questionário sociodemográfico e clínico, a Escala de Coping Religioso/Espiritual Breve (CRE-Breve) e a Escala de Bem-estar Espiritual (EBE) em três coletas distintas, realizadas a cada dois meses. Resultados: Verificou-se que as pessoas com insuficiência renal crônica em hemodiálise fazem uso do enfrentamento religioso e espiritual. A média do CRE positivo foi 3,34, que reflete um escore alto. O EBE total e o EBE religioso associaram-se significativamente à importância dada à religião/espiritualidade (p < 0,001). Conclusão: Os pacientes com insuficiência renal crônica em tratamento hemodialítico entrevistados neste estudo utilizam o coping religioso/espiritual de forma positiva e significativa, da mesma forma que consideram importante a religião/espiritualidade em suas vidas.
Resumen Objetivo: Evaluar la variación temporal y la correlación entre el bienestar espiritual y el afrontamiento religioso/espiritual en personas con insuficiencia renal crónica sometidas a hemodiálisis. Metodología: Estudio descriptivo, analítico y longitudinal. La muestra inicial contó con 118 participantes, pero al final de la recopilación de datos hubo una pérdida de 31, lo que redujo la muestra a 87. Se aplicaron mediante entrevistas el cuestionario sociodemográfico y clínico, la Escala de Coping Religioso/Espiritual Breve (CRE-Breve) y la Escala de Bem-estar Espiritual (EBE). La recopilación de datos se realizó en tres etapas, con intervalos frecuentes de dos meses. Resultados: Se encontró que las personas con insuficiencia renal crónica sometidas a hemodiálisis suelen recurrir al afrontamiento religioso y espiritual. La media positiva de CRE fue de 3,34, lo que reflejó una alta puntuación. Por su parte, el EBE total y el EBE religioso se asociaron significativamente con la importancia que se le da a la religión/espiritualidad (p < 0,001). Conclusiones: Los pacientes con insuficiencia renal crónica sometidos a hemodiálisis utilizan el afrontamiento religioso/espiritual de manera positiva y significativa; así mismo, consideran importante la religión/ espiritualidad en sus vidas.
Abstract Objective: To assess temporal variation and correlation between spiritual well-being and spiritual/religious coping in people with chronic kidney disease on hemodialysis. Methodology: Longitudinal, descriptive and analytical study. Initial sample was composed of 118 participants, but at the end of data collection, there was a loss of 31 participants, which is why the sample was reduced to 87. A socio-demographic and clinical questionnaire, the Brief Spiritual/ Religious Coping Scale (Brief-SRCOPE Scale), and The Spiritual Well-Being Scale (SWB) were applied using interviews. Data collection were carried out in three stages, at two-month frequent intervals. Results: It was found that people with chronic kidney disease on hemodialysis tend to resort spiritual and religious coping. The spiritual/religious coping mean positive was 3.34, reflecting a high score. On the other hand, total SWB and the religious SWB were significantly associated with the importance given to spirituality/religion (p < 0.001). Conclusions: Patients with chronic kidney disease on hemodialysis make use of spiritual/religious coping positively and significantly; likewise, they regard religion/spirituality as important in their lives.
