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Sequence analysis of synthetic oligonucleotides by high-performance liquid anion-exchange chromatography.

van Boom, J H; de Rooy, J F.

J Chromatogr ; 131: 169-77, 1977 Jan 21.

Article in English | MEDLINE | ID: mdl-192745

ABSTRACT

A simple procedure for the sequential anlysis of small oligonucleotides is reported. The method is based on the simultaneous identification and quantitation of monomers released by venom phosphodiesterase digestion of oligonucleotides using high-performance anion-exchange chromatography on Permaphase AAX at room temperature and by applying isocratic elution conditions. In this way, the correct sequence of five oligomers, e.g., r-ACCUCC, r-CUGUU, r-AGGA, d-ATTACC and d-GGTAAT, could easily be established unambiguously.

Subject(s)

Oligonucleotides/analysis , Base Sequence , Chromatography, High Pressure Liquid , Chromatography, Ion Exchange , Methods , Phosphoric Diester Hydrolases

The synthesis of oligoribonucleotides. Part XI. Preparation of ribonucleoside 2'-acetal 3'-esters by selective deacylation.

Reese, C B; Stewart, J C; van Boom, J H; de Leeuw, H P; Nagel, J; de Rooy, J F.

J Chem Soc Perkin 1 ; (10): 934-42, 1975.

Article in English | MEDLINE | ID: mdl-1170189

Subject(s)

Oligonucleotides/chemical synthesis , Ribonucleotides/chemical synthesis , Acetals/chemical synthesis , Esters , Methods

The synthesis of oligoribonucleotides. X. Preparation of 2',3'-cyclic phosphates of ribonucleosides and diribonucleoside phosphates via phosphotriester intermediates.

van Boom, J H; de Rooy, J F; Reese, C B.

J Chem Soc Perkin 1 ; 21: 2513-7, 1973.

Article in English | MEDLINE | ID: mdl-4797713

Subject(s)

Oligonucleotides/chemical synthesis , Ribonucleotides/chemical synthesis , Uracil Nucleotides/chemical synthesis , Cyclization , Methods , Nucleotides, Cyclic/chemical synthesis

ABSTRACT

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