Pattern of gonadotropin secretion along the estrous cycle of C57BL/6 female mice.

The pattern of gonadotropin secretion along the estrous cycle was elegantly described in rats. Less information exists about the pattern of gonadotropin secretion in gonad-intact mice, particularly regarding the follicle-stimulating hormone (FSH). Using serial blood collections from the tail-tip of gonad-intact C57BL/6 mice on the first day of cornification (transition from diestrus to estrus; hereafter called proestrus), we observed that the luteinizing hormone (LH) and FSH surge cannot be consistently detected since only one out of eight females (12%) showed increased LH levels. In contrast, a high percentage of mice (15 out of 21 animals; 71%) exhibited LH and FSH surges on the proestrus when a single serum sample was collected. Mice that exhibited LH and FSH surges on the proestrus showed c-Fos expression in gonadotropin-releasing hormone- (GnRH; 83.4% of co-localization) and kisspeptin-expressing neurons (42.3% of co-localization) of the anteroventral periventricular nucleus (AVPV). Noteworthy, mice perfused on proestrus, but that failed to exhibit LH surge, showed a smaller, but significant expression of c-Fos in GnRH (32.7%) and AVPVKisspeptin (14.0%) neurons. Finally, 96 serial blood samples were collected hourly in eight regular cycling C57BL/6 females to describe the pattern of LH and FSH secretion along the estrous cycle. Small elevations in LH and FSH levels were detected at the time expected for the LH surge. In summary, the present study improves our understanding of the pattern of gonadotropin secretion and the activation of central components of the hypothalamic-pituitary-gonadal axis along the estrous cycle of C57BL/6 female mice.

The effects of auricular acupuncture on vascular parameters on the risk factors for diabetic foot: A randomized clinical trial.

AIMS AND OBJECTIVES: To evaluate the effects of auricular acupuncture on vascular parameters on the risk factors for Diabetic Foot. METHODS: Randomized and masked clinical trial. The sample was composed of 44 individuals with type 2 Diabetes Mellitus and they were randomly assigned to two groups: intervention (n = 22), which received five sessions of auricular acupuncture, and control (n = 22), which did not receive the therapy. Three evaluations were performed: before the intervention; one day after the last acupuncture session and 15 days after the second evaluation. For this, the subject's characterization instrument, the Ankle-Brachial Index measurement, and thermography were used. For data analysis, Mann-Whitney, Wilcoxon, Wilcoxon signed-rank, Fisher's Exact and Chi-Square tests were used. RESULTS: Auriculotherapy provided results in the Ankle-Brachial Index Test, as well as in thermography, which showed significant temperature improvement. CONCLUSIONS: The technique proved to be efficient in improving circulatory conditions and plantar temperature.

Morphological description of the first protozoeal stage of the deep-sea shrimps Aristeus antennatus and Gennadas elegans, with a key.

Accurate information on commercial marine species larvae is key to fisheries science, as their correct identification is the first step towards studying the species' connectivity patterns. In this study, we provide a complete morphological description of the first protozoeal stage of the valued deep-sea blue and red shrimp Aristeus antennatus and of the small mesopelagic shrimp Gennadas elegans. These two larval morphologies previously posed a risk of misidentification, thus hindering the study of A. antennatus larval ecology and dynamics in the context of fisheries science. Using specimens caught in the plankton at various locations in the Northwestern Mediterranean Sea and identification confirmed by molecular methods, the larvae of A. antennatus and G. elegans are distinguished from each other by the ornamentation of the antennula. A possible confusion in previous descriptions of Aristeidae larvae is addressed and a new key for the identification of Dendrobranchiata larvae provided.

Transcutaneous electrical nerve stimulation for the relief of post-partum uterine contraction pain during breast-feeding: a randomized clinical trial.

AIM: To evaluate transcutaneous electrical nerve stimulation (TENS) used to relieve uterine contraction pain during breast-feeding in multiparous women. METHODS: A controlled and randomized clinical trial was performed with 32 post-partum multiparous women who were breast-feeding. The participants were randomized into an experimental (EG, n = 16) and a control group (CG, n = 16). Pain was assessed using the Numerical Rating Scale and the descriptors of the Present Pain Intensity (PPI - McGill) during two sequential feeds. In the first assessment, the post-partum women in the EG and CG were monitored while breast-feeding and questioned regarding the degree of uterine contraction pain. In the second assessment, which was performed at the next feed, the EG used TENS while the CG was monitored, with pain being reassessed following. In the EG, the TENS electrodes were placed between T10-L1 and S2-S4, with a frequency of 100 Hz, a pulse duration of 75 µs and amplitude adjusted to produce a strong and tolerable sensation for 40 min. A descriptive analysis of the data and comparative intra- and intergroup analyses were performed by means of a non-parametrical test, with a significance level P ≤ 0.05. RESULTS: The level of basal pain was 5.56 in the EG and 5.50 in the CG. In the intragroup analysis, the EG (P < 0.0001) and CG (P = 0.04) presented significant pain reduction. In the intergroup analysis, the EG had significant pain reduction (P < 0.01) in comparison to the CG. In the PPI analysis, a non-significant decrease in pain intensity was observed in the EG. CONCLUSION: TENS was found to be effective in reducing post-partum uterine contraction pain during breast-feeding.

[A non-pharmacologic treatment to relieve breast engorgement during lactation: an integrative literature review]. / Terapêutica não-farmacológica para alívio do ingurgitamento mamário durante a lactação: revisão integrativa da literatura.

Breast engorgement affects breastfeeding and leads to early weaning. The literature presents techniques for treating engorgement, but there is no agreement as to which is the best treatment. The objective of this study is to identify and analyze the evidence found in the literature regarding non-pharmacological treatments to relieve breast engorgement symptoms during breastfeeding. We performed an integrative literature review of studies published since 1990, on MEDLINE and LILACS, using the keywords: breastfeeding, lactation disorder and therapy. We found ten studies: two systematic literature reviews, seven randomized controlled trials and one quasi-experimental study. The analyzed studies show conflicting results and there is not enough evidence to recommend the implementation of the evaluated treatments. Further randomized controlled trials are necessary to determine effective therapies for treating breast engorgement.

High-frequency TENS in post-episiotomy pain relief in primiparous puerpere: a randomized, controlled trial.

AIM: We evaluated the effectiveness of high-frequency transcutaneous electrical nerve stimulation (TENS) as a pain relief resource for primiparous puerpere who had experienced natural childbirth with an episiotomy. METHODS: A controlled, randomized clinical study was conducted in a Brazilian maternity ward. Forty puerpere were randomly divided into two groups: TENS high frequency and a no treatment control group. Post-episiotomy pain was assessed in the resting and sitting positions and during ambulation. An 11-point numeric rating scale was performed in three separate evaluations (at the beginning of the study, after 60 min and after 120 min). The McGill pain questionnaire was employed at the beginning and 60 min later. TENS with 100 Hz frequency and 75 µs pulse for 60 min was employed without causing any pain. Four electrodes ware placed in parallel near the episiotomy site, in the area of the pudendal and genitofemoral nerves. RESULTS: An 11-point numeric rating scale and McGill pain questionnaire showed a significant statistical difference in pain reduction in the TENS group, while the control group showed no alteration in the level of discomfort. Hence, high-frequency TENS treatment significantly reduced pain intensity immediately after its use and 60 min later. CONCLUSION: TENS is a safe and viable non-pharmacological analgesic resource to be employed for pain relief post-episiotomy. The routine use of TENS post-episiotomy is recommended.