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J Mass Spectrom ; 46(3): 320-6, 2011 Mar.
Article in English | MEDLINE | ID: mdl-21394848

ABSTRACT

A liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) method for the quantitation of triamcinolone in human plasma after nasal spray application was developed and validated. Betamethasone was used as internal standard (IS). The analytes were extracted by a liquid-liquid procedure and separated on a Zorbax Eclipse XDB C(18) column with a mobile phase composed of 2 mM aqueous ammonium acetate pH 3.2 and acetonitrile (55:45). Selected reaction monitoring was performed using the transitions m/z 435 → 415 and m/z 393 → 373 to quantify triamcinolone acetonide and betamethasone, respectively. Calibration curve was constructed over the range of 20-2000 pg/ml for triamcinolone acetonide. The lower limit of quantitation was 20 pg/ml. The mean RSD values were 4.6% and 5.7% for the intra-run and inter-run precision, respectively. The mean accuracy value was 98.5% and a recovery rate corresponding to 97.5% was achieved. No matrix effect was detected in the samples. The validated method was successfully applied to determine the plasma concentrations of triamcinolone acetonide in healthy volunteers, in a pharmacokinetic study with nasal spray formulation.


Subject(s)
Chromatography, High Pressure Liquid/methods , Spectrometry, Mass, Electrospray Ionization/methods , Triamcinolone/blood , Betamethasone , Humans , Linear Models , Nasal Sprays , Reproducibility of Results , Sensitivity and Specificity , Tandem Mass Spectrometry/methods , Triamcinolone/administration & dosage , Triamcinolone/pharmacokinetics
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