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1.
Appl Biochem Biotechnol ; 194(8): 3365-3383, 2022 Aug.
Article in English | MEDLINE | ID: mdl-35357662

ABSTRACT

The aim of this study was to evaluate the theoretical potential of methane production of the food waste generated by a university restaurant, as well as to verify the influence of the fruit and vegetable waste in the feeding composition of an anaerobic bioreactor treating this type of waste. Four feeding compositions combining three fractions of the food waste (fruit and vegetable fraction, soy protein and beans fraction, and rice fraction) at different concentrations were tested in anaerobic processes lasting 10 and 30 days. Additionally, a study of the theoretical potential of methane production from each fraction that composes the food waste was carried out, as well as the evaluation of the specific methanogenic activity of the anaerobic sludge. Despite its low theoretical potential of methane production (0.037 LCH4/g), the presence of the fruit and vegetable mixture in three of the feeding compositions led to greater organic matter degradation (above 69%) and CH4 yields (above 0.20 LCH4/gVS) in both periods tested, in comparison with the achieved by the feeding composition lacking this fraction. The results suggest that the presence of the fruit and vegetable mixture contributed with the supplementation of micro- and macroelements to the anaerobic sludge during the digestion of food waste.


Subject(s)
Refuse Disposal , Vegetables , Anaerobiosis , Bioreactors , Food , Fruit/metabolism , Humans , Methane , Refuse Disposal/methods , Restaurants , Sewage , Universities , Vegetables/metabolism
2.
Bioresour Technol ; 200: 528-34, 2016 Jan.
Article in English | MEDLINE | ID: mdl-26524251

ABSTRACT

The objective of this study was to select a concentration of CO2 absorbents to supplement Spirulina sp. LEB 18 cultivation and to evaluate the effect of these compounds on the growth and production of macromolecules. Three initial biomass concentrations (X0), eight concentrations of monoethanolamine (MEA), and three NaOH concentrations were tested. The selected MEA concentrations did not inhibit the growth of Spirulina and doubled the dissolved inorganic carbon concentration in the assay medium in relation to the concentration of NaOH. The protein concentration in the biomass grown with MEA was, on average, 17% higher than that obtained with NaOH. Thus, it was found that MEA did not reduce the productivity of Spirulina sp. LEB 18, and its use can be further explored as a means for converting the carbon dissolved in the medium to biomolecules.


Subject(s)
Biomass , Carbon Dioxide/chemistry , Macromolecular Substances/metabolism , Spirulina/growth & development , Carbon/chemistry , Carbon/pharmacology , Ethanolamine/pharmacology , Hydrogen/chemistry , Hydrogen-Ion Concentration , Nitrogen/chemistry , Organic Chemicals/chemistry , Photobioreactors , Sodium Hydroxide/chemistry , Temperature
3.
Bioresour Technol ; 192: 321-7, 2015 Sep.
Article in English | MEDLINE | ID: mdl-26051496

ABSTRACT

The chemical absorption of carbon dioxide (CO2) is a technique used for the mitigation of the greenhouse effect. However, this process consumes high amounts of energy to regenerate the absorbent and to separate the CO2. CO2 removal by microalgae can be obtained via the photosynthesis process. The objective of this study was to investigate the cultivation and the macromolecules production by Spirulina sp. LEB 18 with the addition of monoethanolamine (MEA) and CO2. In the cultivation with MEA, were obtained higher results of specific growth rate, biomass productivity, CO2 biofixation, CO2 use efficiency, and lower generation time. Besides this, the carbohydrate concentration obtained at the end of this assay was approximately 96.0% higher than the control assay. Therefore, Spirulina can be produced using medium recycle and the addition of MEA, thereby promoting the reduction of CO2 emissions and showing potential for areas that require higher concentrations of carbohydrates, such as in bioethanol production.


Subject(s)
Biotechnology/methods , Carbon Dioxide/metabolism , Spirulina/growth & development , Spirulina/metabolism , Biomass , Carbohydrate Metabolism , Carbohydrates/analysis , Carbon Dioxide/chemistry , Ethanolamine/pharmacology , Spirulina/drug effects
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