ABSTRACT
OBJECTIVE: To analyze the participation of glutathione-S-transferase (GST) M1 and T1 polymorphisms associated or not with protein p53 polymorphism at codon 72 and in the presence of HPV in the carcinogenesis of uterine cervix adenocarcinoma. METHODS: Forty-three samples of uterine cervix adenocarcinoma were studied and 86 samples of endocervical cells of women without tumors formed the control group. The presence of HPV was determined in order to genotype the isoforms of p53 at codon 72, GSTM1, GSTM1*0, GSTT1 and GSTT1*0 which were evaluated by the PCR method. RESULTS: HPV was present in 97.67% of the adenocarcinoma cases and in 31.40% of the control group. Statistical analysis showed differences (p = 0.001) and an OR of 113.3 (CI 95%: 13.67-947.14). GSTT1 and GSTT1*0 analysis showed a significant difference between the groups (p = 0.001) with an OR of 4.58 (CI 95%: 2.041-10.28) (p < 0.001) for the presence of GSTT1*0. When it was associated with HPV OR was 6.6 (CI 95%: 0.04-0.50). Analyses of p53 and GSTM1 and GSTM1*0 either alone or associated with HPV were not significant. CONCLUSION: The presence of GSTT1*0 increased the risk for uterine cervix adenocarcinoma development while the allele GSTT1 had a protective action. The other isoforms did not appear to participate in the carcinogenesis of uterine cervix adenocarcinoma.
Subject(s)
Adenocarcinoma/genetics , Alphapapillomavirus/isolation & purification , Glutathione Transferase/genetics , Polymorphism, Single Nucleotide/genetics , Uterine Cervical Neoplasms/genetics , Adult , Aged , Aged, 80 and over , Case-Control Studies , Cohort Studies , Female , Genes, p53/genetics , Humans , Middle Aged , Odds Ratio , Young AdultABSTRACT
PURPOSE OF INVESTIGATION: To analyze the results of a statewide screening program inaugurated in 2002 in Mato Grosso do Sul, METHODS: Retrospective audit of the screening results of 8,477 pregnant women for 11 diseases in 19 tests. Local health centers of the Brazilian Unified System of Health/State Program of Pregnant Protection (SUS/PEPG) and central reference processing laboratories, both in and out of state were involved. Pregnant women were referred to the program by medical staff principally at local health centers, between November 2002 and February 2003. Primary screening with filter-paper blood samples was carried out with positives being resampled and sera appropriately tested. RESULTS: The detailed test results are given below; the most frequent diseases serodiagnosed were hepatitis B, syphilis, HIV and Chagas' disease. One case of maternal phenylketonouria (PKU) was encountered. CONCLUSION: The diagnosis of 11 diseases of importance for mothers and their offspring provides an epidemiological profile of Brazil, but must be matched by opportunities to elaborate new control strategies and improve health care during pregnancy.
