ABSTRACT
BACKGROUND AND OBJECTIVE: This study assessed the effects of the local use of Saccharomyces cerevisiae as monotherapy and as an adjuvant to the mechanical treatment of ligature-induced periodontitis in rats. MATERIAL AND METHODS: Periodontitis was induced in 72 rats via the installation of a ligature around the mandibular first molar. After 7 d, the ligature was removed and the rats were placed in one of the following groups: no treatment (C; n = 18); scaling and root planing (SRP; n = 18); local irrigation with probiotics (PRO; n = 18); and SRP followed by local irrigation with probiotics (SRP/PRO; n = 18). Six rats from each group were killed at 7, 15 and 30 d. The histological characteristics, alveolar bone loss (ABL) and immunolabeling of tumor necrosis factor alpha (TNF-α), interleukin-1beta (IL-1ß), interleukin-10 (IL-10) and TRAP on the furcation area of the first molar were assessed. RESULTS: The PRO group showed features of acceleration of the tissue-repair process during the entire experiment. On day 15, there was less ABL in the SRP/PRO group compared with the C group. There were fewer TRAP-positive cells in the SRP and SRP/PRO groups at 30 d. There was less immunostaining for TNF-α in the PRO and SRP/PRO groups and less immunostaining for IL-1ß in the PRO group. However, there was more immunostaining for IL-10 in the PRO group on day 15. CONCLUSION: Local use of the probiotic did not result in any adverse effects on periodontal tissues. When used as monotherapy or as an adjuvant, the probiotic was effective at controlling periodontitis in rats.
Subject(s)
Periodontitis , Alveolar Bone Loss , Animals , Dental Scaling , Ligation , Probiotics , Rats , Rats, Wistar , Saccharomyces cerevisiaeABSTRACT
In the present study we investigated the presence of spirochetes in the subgingival plaque of 66 boys and girls aged 5 to 10 years. A total of 264 samples were removed from 4 different sites using wooden wedges held with hemostatic pliers and introduced into tubes containing 0.6 ml reduced transport fluid (RTF). After dispersal of the material, smears were prepared and stained by the Fontana-Tribondeau and Giemsa methods for spirochete visualization. Slides were examined under a binocular light microscope with a 100X objective and at least 200 microorganisms were counted, including spirochetes. Spirochetes were detected in 27.27% of the samples and were absent in 72.73% of them, i.e., 65.15% of the children harbored spirochetes in at least one of the sites studied. The relative frequency of percentage of spirochetes observed was quite low in 6-year old children (9.09%), and was higher in 7- and 10-year old children (41.76% and 36.62%, respectively) than in the remaining age groups studied. The spirochete-age correlation was statistically significant (P < 0.01).
Subject(s)
Dental Plaque/microbiology , Spirochaetales/isolation & purification , Age Distribution , Brazil , Chi-Square Distribution , Child , Child, Preschool , Female , Humans , MaleABSTRACT
The gingival reaction to 4 different suture materials used in periodontal surgery was studied in 36 patients. The gingiva was sutured prior to surgery and biopsies were taken at 3, 7 and 14 days to observe the tissue reaction. The histological examination showed that silk caused the most intense and longest inflammatory response. Polyester and perlon provoked shorter, less intense tissue reactions than silk, and nylon caused the least inflammatory response, with earlier tissue repair.
Subject(s)
Dental Materials/adverse effects , Gingiva/drug effects , Insect Proteins , Sutures , Adolescent , Adult , Caprolactam/adverse effects , Caprolactam/analogs & derivatives , Female , Humans , Male , Middle Aged , Nylons/adverse effects , Polyesters/adverse effects , Polymers/adverse effects , Proteins/adverse effects , SilkABSTRACT
Three hundred and twenty samples of subgingival plaque were obtained from 80 caucasian girls, ranging from 10 to 13 years of age. The samples were analyzed to verify the influence of age upon colonization of the gingival sulcus by microorganisms potentially pathogenic to the periodontal tissues. The gingival and plaque status were evaluated through the gingival index (GI) and plaque index (PlI) and the microflora was assessed by the enzymatic method benzoyl-arginine-naphthylamide (BANA). The results of the BANA test were positive for 62.50% of the tested individuals and 40% of the examined sites. The influence of age was statistically significant on BANA reactivity, and the number of positive sites was greater at 11 (57.5%) than at 12 years (28.8%).
Subject(s)
Benzoylarginine-2-Naphthylamide/metabolism , Dental Plaque/microbiology , Spirochaetales/isolation & purification , Adolescent , Age Factors , Bacteriological Techniques , Brazil , Chi-Square Distribution , Child , Female , Humans , Spirochaetales/metabolismABSTRACT
The effect of sodium bicarbonate jet (Profident System) on the gingival tissue was tested with comparison of application from 2 different angles: the angulation recommended by the manufacturer and ponted to the occlusal surface. The results showed a definite influence of the angulation on tissue trauma, with lesser degrees of injury when the modified angulation was applied.
