ABSTRACT
Here, the modulation of enzyme activity is presented by protein-imprinted nanoparticles produced using a solid-phase approach. Using trypsin as target, binding of the nanoparticles to the enzyme results in its inhibition or in stabilization, depending on the orientation of the immobilized enzyme used during imprinting.
Subject(s)
Enzymes, Immobilized/metabolism , Molecular Imprinting/methods , Nanoparticles/chemistry , Trypsin/metabolism , Biocompatible Materials/chemical synthesis , Biocompatible Materials/chemistry , Biocompatible Materials/metabolism , Enzymes, Immobilized/chemistry , Nanotechnology/methods , Surface Plasmon Resonance , Trypsin/chemistryABSTRACT
A new ELISA- (enzyme-linked immunosorbent assay)-like assay is demonstrated in which no elements of biological origin are used for molecular recognition or signaling. Composite imprinted nanoparticles that contain a catalytic core and which are synthesized by using a solid-phase approach can simultaneously act as recognition/signaling elements, and be used with minimal modifications to standard assay protocols. This assay provides a new route towards replacement of unstable biomolecules in immunoassays.
