ABSTRACT
Specific identification of oestrid larvae is usually problematic not only when using morphobiometric features, but also when applying molecular criteria, since very few molecular markers have been described for this group of flies. New molecular markers for oestrid are needed for more reliable species identification, diagnostic purposes, and epidemiological surveys; moreover, they can help in phylogenetic reconstruction. Here, we report the characterization of COI, 28S rDNA, ITS1, and ITS2 in Cephenemyia stimulator from roe deer and in Cephenemyia auribarbis and Pharyngomyia picta from red deer. The COI and 28S rDNA are very uniform in length, while the ITSs sequences are highly variable at both intraspecific and interspecific levels. The described ITSs sequences were longer than those described for other dipteran species by the presence of simple repeats and tandem repeat sequences. In C. auribarbis both ITS1 and ITS2 appeared as two variants, one short and the other long. In general, the analyzed markers present low intraspecific genetic variation and high interspecific variation. ITSs showed the greatest amount of intraspecific and interspecific variation. Phylogenetic analysis demonstrated that the characterized sequences differentiate the species and genera of Oestridae.
Subject(s)
Biomarkers/analysis , Diptera/physiology , Myiasis/veterinary , Animals , France , Insect Proteins/analysis , Myiasis/diagnosis , Myiasis/parasitology , SpainABSTRACT
INTRODUCCIÓN: La praxis de la odontología requiere el desarrollo adecuado de las habilidades de comunicación, competencia transversal que ha de estar garantizada durante los estudios de grado. Esto supone un reto institucional, por cuanto las facultades han de poner los mecanismos académicos para evaluar el nivel de desarrollo de dichas habilidades. MÉTODOS: Se plantea un plan integral de carácter colaborativo para el desarrollo de las habilidades comunicativas en el grado en Odontología de la Universidad del País Vasco/Euskal Herriko Unibertsitatea, que contempla cuatro fases: a) recogida y socialización de la información sobre el desarrollo de la competencia; b) diseño de desarrollo trasversal y vertical de la competencia; c) implementación y análisis de resultados; y d) recogida de evidencias del proceso y divulgación de los resultados. RESULTADOS: Tras la detección de las fortalezas y debilidades, se definieron 40 habilidades comunicacionales, estructuradas en cinco bloques (comunicación oral con pacientes/familiares, comunicación oral con otros profesionales, comunicación escrita con pacientes/familiares, comunicación escrita con otros profesionales sanitarios y técnicas de comunicación odontólogo-paciente) y tres niveles de desempeño (identificación de la información, desempeño con alta supervisión y desempeño autónomo con moderada supervisión). Para su desarrollo, se diseñaron 19 actividades formativas y de evaluación, actualmente en implementación. CONCLUSIÓN: El plan se está desarrollando con éxito, y las evidencias del proceso, recogidas en un portafolio, servirán para el seguimiento de la titulación
INTRODUCTION: The practice of dentistry requires the proper development of communication skills, which must be guaranteed during undergraduate studies. This is an institutional challenge, because the schools have to put the academic mechanisms to evaluate the level of development of these skills. METHODS: The University of the Basque Country (UPV/EHU) is developing a comprehensive collaborative plan that includes four phases: a) collection and socialization of information on the current situation of the development of communication skills; b) design of a training plan to improve these skills, c) training plan implementation and results assessment, and d) gathering evidence of the process and dissemination of the results. RESULTS: After the detection of strengths and weaknesses in communication, we defined a total of 40 communication skills, structured in five blocks (oral communication with patients/family members; oral communication with other professionals; written communication with patients/family members; written communication with other health professionals, and dentist-patient communication techniques) and three levels of performance (identifying information, performance with high supervision, autonomous with moderate supervision). For its development, 19 training and evaluation activities were designed, currently being implemented. CONCLUSION: The plan is being developed successfully and the evidence of the process, collected in a portfolio, will be used to monitor the degree
Subject(s)
Humans , Dental Care/psychology , Education, Dental/organization & administration , Health Communication/methods , Curriculum/trends , Social Skills , Dentist-Patient Relations , Students, Dental/psychology , Interprofessional Relations , Surveys and Questionnaires/statistics & numerical data , Organizational InnovationABSTRACT
INTRODUCTION: A new technique was performed for the removal of an amalgam tattoo (AT), consisting in mucoabrasion combined with a free connective tissue graft (FCTG). CASE PRESENTATION: A 59-year-old female presented with a large AT involving the alveolar mucosa and gingiva between teeth #7 and #8, where endodontic treatment and apicoectomy was performed 21 years ago. To avoid compromising esthetics, the lesion was managed with a new technique including mucoabrasion and an FCTG. At the 53-month follow-up, the patient exhibited a naturally appearing soft tissue with no evidence of the AT. CONCLUSION: When performing a one-stage treatment of removing ATs in areas of high esthetic demand, it is possible to achieve good results and ideal gingival color.
Subject(s)
Dental Amalgam , Gingival Diseases , Pigmentation Disorders , Tattooing , Connective Tissue , Female , Gingival Diseases/therapy , Humans , Middle Aged , Pigmentation Disorders/therapyABSTRACT
OBJECTIVES: The aim of this study was to assess the clinical outcomes of a nonsurgical treatment protocol for peri-implantitis and to evaluate the influence that some factors could have on the results of this treatment. MATERIAL AND METHODS: In this retrospective case series, patients with at least one implant with peri-implantitis, treated with a nonsurgical protocol and with a one-year follow-up, were included. Clinical parameters (probing depth, recession, bleeding, and/or suppuration on probing) were collected at baseline, 6 weeks, 3, 6, and 12 months. Radiographic bone levels were assessed in periapical radiographs taken at baseline and 12 months. An analysis of the factors significantly associated with marginal bone level changes was performed. Descriptive and analytic statistics were carried out at the patient and at the implant level, as well as a multiple linear regression analysis. RESULTS: Data from 37 patients with 70 implants were analyzed. There was a general and consistent improvement from baseline compared to all follow-up points in the clinical variables. A significant radiographic bone gain of 0.91 mm was observed. The factors significantly associated were baseline plaque index, sex, and age at patient level, and type of prosthesis and implant location at implant level. CONCLUSIONS: Within the limitations of this study, this nonsurgical treatment protocol might be considered as a first treatment option for peri-implantitis. More studies are needed, with larger sample sizes and longer follow-ups, to confirm these outcomes and their long-term stability.
Subject(s)
Peri-Implantitis , Clinical Protocols , Dental Plaque Index , Humans , Periodontal Index , Retrospective StudiesABSTRACT
OBJETIVO: Adaptar al español la escala de evaluación del comportamiento del niño durante la inducción anestésica Induction Compliance Checklist (ICC). MÉTODO: Estudio observacional de validación realizado a una muestra de 81 niños de 2 a 12 años operados en una Unidad de Cirugía Ambulatoria de un hospital pediátrico de Barcelona. Adaptación por el método de traducción-retrotraducción de la herramienta y análisis de validez y fiabilidad de la escala. RESULTADOS: Se garantizó la validez aparente de la herramienta mediante un grupo de discusión y se evaluó la fiabilidad interobservadores; el índice de correlación intraclase obtenido fue de r = 0,956. CONCLUSIONES: La escala ICC validada para la población española puede ser una herramienta eficaz para la evaluación de las actividades prequirúrgicas que se realizan para minimizar la ansiedad de los niños. La ICC es una escala fácil de usar y de completar por el personal de quirófano y que aportaría gran información en cuanto al comportamiento del niño durante la inducción anestésica
OBJECTIVE: Cross-cultural adaptation into Spanish of the Induction Compliance Checklist (ICC) for assessing children's behaviour during induction of anaesthesia. Method: A descriptive cross-sectional observational study was conducted on a sample of 81 children aged 2 to 12 years operated in an ambulatory surgery unit of a paediatric hospital in Barcelona. Adaptation by translation-back translation of the tool and analysis of the scale's validity and reliability. RESULTS: Face validity of the tool was guaranteed through a discussion group and inter-observer reliability was evaluated, obtaining an intraclass correlation index of r = 0.956. CONCLUSIONS: The ICC scale validated for the Spanish population can be an effective tool for the presurgical evaluation of activities carried out to minimise children's anxiety. The ICC is an easy-to-use scale completed by operating room staff in one minute and would provide important information about children's behaviour, specifically during induction
Subject(s)
Humans , Child, Preschool , Child , Cross-Cultural Comparison , Child Behavior , Anesthesia , Nursing Assessment , Data Analysis , Premedication/nursing , Test Anxiety ScaleABSTRACT
OBJECTIVE: Cross-cultural adaptation into Spanish of the Induction Compliance Checklist (ICC) for assessing children's behaviour during induction of anaesthesia. METHOD: A descriptive cross-sectional observational study was conducted on a sample of 81 children aged 2 to 12 years operated in an ambulatory surgery unit of a paediatric hospital in Barcelona. Adaptation by translation-back translation of the tool and analysis of the scale's validity and reliability. RESULTS: Face validity of the tool was guaranteed through a discussion group and inter-observer reliability was evaluated, obtaining an intraclass correlation index of r = 0.956. CONCLUSIONS: The ICC scale validated for the Spanish population can be an effective tool for the presurgical evaluation of activities carried out to minimise children's anxiety. The ICC is an easy-to-use scale completed by operating room staff in one minute and would provide important information about children's behaviour, specifically during induction.
ABSTRACT
BACKGROUND: Connective tissue graft (CTG) is considered as the gold standard for the treatment of gingival recessions (GR). There are few studies assessing the complications that can arise in the donor site when harvesting a connective tissue graft (CTG) and how the harvesting technique can influence those complications. MATERIAL AND METHODS: A retrospective clinical study was carried out in order to compare the complications observed in 40 patients with Miller class I, II and III GR ≥ 3 mm, after using the trap-door technique (TD) in the control group and a newly described technique, the "UPV/EHU technique", in the test group. Patients were consecutively allocated to each treatment group. Patients were monitored 14 days after surgery in order to evaluate post-operative complications in the donor site: presence of pain (P), bleeding (B), infection (I) and necrosis > 30%. RESULTS: Although morbidity was observed in both groups, it was less important in the test group (no pain and minimal pain in 30% and 35% of the cases, respectively, and absence of bleeding or infection and necrosis >30% in only 5% of the cases). CONCLUSIONS: Within the limits of this study, this newly described "UPV/EHU technique" should be considered as a treatment option when harvesting a CTG, with minimal morbidity for patients. Key words:Connective tissue graft, pain, gingival recessions, wound healing, cosmetic periodontal plastic surgery, trap-door technique, "UPV/EHU technique".
ABSTRACT
Participation--defined as engagement in life situations, including leisure and recreational activities--is associated with the improvement of people with disabilities' quality of life. Several specific instruments assess leisure, but none of them has been adapted to the Spanish context. The goal of this study is to adapt and validate the Spanish version of the Leisure Assessment Inventory (LAI; B. A. Hawkins, P. Ardovino, N. B. Rogers, A. Foose, & N. Olsen, 2002 ). The adaptation of the original version of the LAI was carried out through translation and backward translation, and the validity of the instrument was analyzed. Descriptive analyses (means and standard deviations) were conducted for each LAI index. Construct validity was assessed through Pearson's product-moment correlation among the diverse LAI indexes, and convergent-discriminant validity through the correlation of the diverse indexes and the measures of quality of life. Results show that the LAI indexes are valid measures of the attributes of leisure behavior (participation, preference, interest, and barriers). This study provides a valid instrument to assess the participation profile of adults with disabilities in leisure activities.
Subject(s)
Developmental Disabilities/psychology , Intellectual Disability/psychology , Leisure Activities , Adolescent , Adult , Cerebral Palsy/psychology , Female , Humans , Male , Middle Aged , Quality of Life/psychology , Reproducibility of Results , Spain , Surveys and Questionnaires/standards , Translating , Young AdultABSTRACT
Se analiza qué mediadores ambientales simbólicos son másfrecuentes en los contextos de hospitalización infantil,entendidos como estímulos ambientales asociados, a travésde vívencias propias o vicarias, a experiencias emocionales determinadas que pueden incidir en la experiencia emocional de los pacientes. Existe un amplio margen de mejora de estos aspectos en los entornos actuales de la hospitalización infantil y los profesionaies de enfermeria pediátrica asumenfunciones de control de los mediadores ambientales simbólicos en los citados procesos de hospitalizaciónn. La investigación recogida en este trabajo se ha realizado con ayuda financiera de la Junta de Castilla y León
In the work which methods and results we shall later describe, we have tried to indicate, whith symbolic erivironmental mediators appear most frequently in the contexts of child hospitalisation. By symbolic environmental mediator, we understand the stimulus signals of the environment associated, by virtue of personal or vicarious experiences, to certainemotional experiences -this is, signals capable of affecting the patients' emotional experience. From our work it follows that there exists a wide margin of improvement of these aspects in the current settings of child hospitalization, and that it is the paediatric nursing professibnals who, in an emergent way, are assuming functions of control of thesymbolic environmental mediators in the mentined processes of hospitalisation
