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1.
J Med Chem ; 63(18): 10330-10338, 2020 09 24.
Article in English | MEDLINE | ID: mdl-32866001

ABSTRACT

The protein FKBP52 is a steroid hormone receptor coactivator likely involved in neurodegenerative disease. A series of small, water-soluble, bioinspired, pseudopeptidic fluorescent ligands for the FK1 domain of this protein are described. The design is such that engulfing of the ligand in the pocket of this domain is accompanied by hydrogen-bonding of the dansyl chromophore which functions as both an integral part of the ligand and a fluorescent reporter. Binding is concomitant with a significant wavelength shift and an enhancement of the ligand fluorescence signal. Excitation of FK1 domain native tryptophan residues in the presence of bound ligand results in Förster resonance energy transfer. Variation of key ligand residues within the short sequence was undertaken, and the interaction of the resulting library with the protein was measured by techniques including isothermal calorimetry analysis, fluorescence, and FRET quenching, and a range of Kd values were determined. Cocrystallization of a protein ligand complex at 2.30 Å resolution provided detailed information at the atomic scale, while also providing insight into native substrate binding.


Subject(s)
Fluorescent Dyes/metabolism , Oligopeptides/metabolism , Tacrolimus Binding Proteins/metabolism , Fluorescence Resonance Energy Transfer , Fluorescent Dyes/chemical synthesis , Humans , Ligands , Oligopeptides/chemical synthesis , Protein Binding , Protein Domains , Tacrolimus Binding Proteins/chemistry
2.
PLoS One ; 12(12): e0189049, 2017.
Article in English | MEDLINE | ID: mdl-29206236

ABSTRACT

The Phospholipase D (PLD) superfamily of proteins includes a group of enzymes with nuclease activity on various nucleic acid substrates. Here, with the aim of better understanding the substrate specificity determinants in this subfamily, we have characterised the enzymatic activity and the crystal structure of NucT, a nuclease implicated in Helicobacter pylori purine salvage and natural transformation and compared them to those of its bacterial and mammalian homologues. NucT exhibits an endonuclease activity with a strong preference for single stranded nucleic acids substrates. We identified histidine124 as essential for the catalytic activity of the protein. Comparison of the NucT crystal structure at 1.58 Å resolution reported here with those of other members of the sub-family suggests that the specificity of NucT for single-stranded nucleic acids is provided by the width of a positively charged groove giving access to the catalytic site.


Subject(s)
Endonucleases/metabolism , Helicobacter pylori/enzymology , Amino Acid Sequence , Crystallography, X-Ray , Endonucleases/chemistry , Protein Conformation , Sequence Homology, Amino Acid , Substrate Specificity
3.
Biochem Soc Trans ; 43(5): 819-24, 2015 Oct.
Article in English | MEDLINE | ID: mdl-26517888

ABSTRACT

We have designed a new family of artificial proteins, named αRep, based on HEAT (acronym for Huntingtin, elongation factor 3 (EF3), protein pphosphatase 2A (PP2A), yeast kinase Tor1) repeat proteins containing an α-helical repeated motif. The sequence of the repeated motifs, first identified in a thermostable archae protein was optimized using a consensus design strategy and used for the construction of a library of artificial proteins. All proteins from this library share the same general fold but differ both in the number of repeats and in five highly randomized amino acid positions within each repeat. The randomized side chains altogether provide a hypervariable surface on αRep variants. Sequences from this library are efficiently expressed as soluble, folded and very stable proteins. αRep binders with high affinity for various protein targets were selected by phage display. Low micromolar to nanomolar dissociation constants between partners were measured and the structures of several complexes (specific αRep/protein target) were solved by X-ray crystallography. Using GFP as a model target, it was demonstrated that αReps can be used as bait in pull-down experiments. αReps can be expressed in eukaryotic cells and specifically interact with their target addressed to different cell compartments.


Subject(s)
Crystallization/methods , Gene Knockdown Techniques , Models, Molecular , Protein Engineering , Recombinant Fusion Proteins/chemistry , Repetitive Sequences, Amino Acid , Animals , Binding Sites , Cell Line , Consensus Sequence , Gene Library , Humans , Peptide Library , Protein Conformation , Protein Folding , Protein Stability , Protein Structure, Secondary , Recombinant Fusion Proteins/metabolism
4.
J Bacteriol ; 195(18): 4121-8, 2013 Sep.
Article in English | MEDLINE | ID: mdl-23852866

ABSTRACT

We have previously described the posttranslational modification of pore-forming small proteins of Corynebacterium by mycolic acid, a very-long-chain α-alkyl and ß-hydroxy fatty acid. Using a combination of chemical analyses and mass spectrometry, we identified the mycoloyl transferase (Myt) that catalyzes the transfer of the fatty acid residue to yield O-acylated polypeptides. Inactivation of corynomycoloyl transferase C (cg0413 [Corynebacterium glutamicum mytC {CgmytC}]), one of the six Cgmyt genes of C. glutamicum, specifically abolished the O-modification of the pore-forming proteins PorA and PorH, which is critical for their biological activity. Expectedly, complementation of the cg0413 mutant with either the wild-type gene or its orthologues from Corynebacterium diphtheriae and Rhodococcus, but not Nocardia, fully restored the O-acylation of the porins. Consistently, the three-dimensional structure of CgMytC showed the presence of a unique loop that is absent from enzymes that transfer mycoloyl residues onto both trehalose and the cell wall arabinogalactan. These data suggest the implication of this structure in the enzyme specificity for protein instead of carbohydrate.


Subject(s)
Acyltransferases/metabolism , Bacterial Proteins/metabolism , Corynebacterium glutamicum/enzymology , Mycolic Acids/metabolism , Peptides/metabolism , Porins/metabolism , Acylation , Acyltransferases/chemistry , Acyltransferases/genetics , Amino Acid Sequence , Bacterial Proteins/chemistry , Bacterial Proteins/genetics , Corynebacterium glutamicum/genetics , Corynebacterium glutamicum/metabolism , Models, Molecular , Molecular Sequence Data , Porins/genetics , Substrate Specificity
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