ABSTRACT
BipA is a universally conserved prokaryotic GTPase that exhibits differential ribosome association in response to stress-related events. It is a member of the translation factor family of GTPases along with EF-G and LepA. BipA has five domains. The N-terminal region of the protein, consisting of GTPase and beta-barrel domains, is common to all translational GTPases. BipA domains III and V have structural counterparts in EF-G and LepA. However, the C-terminal domain (CTD) of the protein is unique to the BipA family. To investigate how the individual domains of BipA contribute to the biological properties of the protein, deletion constructs were designed and their GTP hydrolysis and ribosome binding properties assessed. Data presented show that removal of the CTD abolishes the ability of BipA to bind to the ribosome and that ribosome complex formation requires the surface provided by domains III and V and the CTD. Additional mutational analysis was used to outline the BipA-70S interaction surface extending across these domains. Steady state kinetic analyses revealed that successive truncation of domains from the C-terminus resulted in a significant increase in the intrinsic GTP hydrolysis rate and a loss of ribosome-stimulated GTPase activity. These results indicate that, similar to other translational GTPases, the ribosome binding and GTPase activities of BipA are tightly coupled. Such intermolecular regulation likely plays a role in the differential ribosome binding by the protein.
Subject(s)
GTP Phosphohydrolases/metabolism , Guanosine Triphosphate/metabolism , Protein Biosynthesis , Ribosomes/metabolism , Circular Dichroism , Cloning, Molecular , Electrophoresis, Polyacrylamide Gel , GTP Phosphohydrolases/chemistry , GTP Phosphohydrolases/genetics , Hydrolysis , Models, Molecular , Mutagenesis, Site-Directed , Protein Binding , Recombinant Proteins/genetics , Recombinant Proteins/metabolismABSTRACT
Mammalian nucleostemin (NS) is a nucleolar guanosine triphosphate-binding protein implicated in cell cycle progression, stem cell proliferation, and ribosome assembly. Drosophila melanogaster contains a four-member nucleostemin family (NS1-4). NS1 is the closest orthologue to human NS; it shares 33% identity and 67% similarity with human NS. We show that NS1 has intrinsic GTPase and ATPase activity and that it is present within nucleoli of most larval and adult cells. Endogenous NS1 and lightly expressed green fluorescent protein (GFP)-NS1 enrich within the nucleolar granular regions as expected, whereas overexpressed GFP-NS1 localized throughout the nucleolus and nucleoplasm, and to several transcriptionally active interbands of polytene chromosomes. Severe overexpression correlated with the appearance of melanotic tumors and larval/pupal lethality. Depletion of 60% of NS1 transcripts also lead to larval and pupal lethality. NS1 protein depletion>95 correlated with the loss of imaginal island (precursor) cells in the larval midgut and to an apparent block in the nucleolar release of large ribosomal subunits in terminally differentiated larval midgut polyploid cells. Ultrastructural examination of larval Malpighian tubule cells depleted for NS1 showed a loss of cytoplasmic ribosomes and a concomitant appearance of cytoplasmic preautophagosomes and lysosomes. We interpret the appearance of these structures as indicators of cell stress response.
Subject(s)
Adenosine Triphosphate/metabolism , Drosophila Proteins/physiology , GTP-Binding Proteins/physiology , Guanosine Triphosphate/metabolism , Intestines/cytology , Ribosome Subunits, Large/metabolism , Amino Acid Sequence , Animals , Cell Differentiation , Cell Nucleolus/enzymology , Chromosomes/ultrastructure , Conserved Sequence , Drosophila Proteins/deficiency , Drosophila Proteins/genetics , GTP-Binding Proteins/deficiency , GTP-Binding Proteins/genetics , Gene Deletion , Gene Knockdown Techniques , Genes, Reporter , Intestines/enzymology , Intestines/growth & development , Larva , Lysosomes/physiology , Malpighian Tubules/enzymology , Malpighian Tubules/ultrastructure , Molecular Sequence Data , Neoplasms, Experimental/genetics , Phagosomes/physiology , Pupa , RNA Interference , Sequence Alignment , Sequence Homology, Amino AcidABSTRACT
BipA is a highly conserved prokaryotic GTPase that functions to influence numerous cellular processes in bacteria. In Escherichia coli and Salmonella enterica serovar Typhimurium, BipA has been implicated in controlling bacterial motility, modulating attachment and effacement processes, and upregulating the expression of virulence genes and is also responsible for avoidance of host defense mechanisms. In addition, BipA is thought to be involved in bacterial stress responses, such as those associated with virulence, temperature, and symbiosis. Thus, BipA is necessary for securing bacterial survival and successful invasion of the host. Steady-state kinetic analysis and pelleting assays were used to assess the GTPase and ribosome-binding properties of S. enterica BipA. Under normal bacterial growth, BipA associates with the ribosome in the GTP-bound state. However, using sucrose density gradients, we demonstrate that the association of BipA and the ribosome is altered under stress conditions in bacteria similar to those experienced during virulence. The data show that this differential binding is brought about by the presence of ppGpp, an alarmone that signals the onset of stress-related events in bacteria.