ABSTRACT
It has been shown that infection with high-risk human papillomaviruses (HR-HPV) is related to the development of cervical cancer. The persistence of the virus in intra-epithelial lesions of cervix uteri (SILs) is the basis for the application of HPV testing for screening and management of patients. Most infections by HR-HPVs resolve spontaneously, however, and do not progress to dysplasia or cancer. p16INK4a is a useful biomarker of cervical intra-epithelial neoplasia and could be a marker for the progression of low-grade squamous intra-epithelial lesions (LSILs) to high-grade squamous intra-epithelial lesions (HSILs), because it correlates independently with increasing SIL grade. We conducted a preliminary histological study of 28 patients diagnosed with LSIL, HSIL or nondysplastic epithelium (NDE) from whom 28 biopsies of uterine cervix and 28 endocervical brushed biopsies were taken. Argyrophilic nucleolar organizer region (AgNOR) and p16INK4a assays were performed on the biopsies, and endocervical brushings were used for HPV typing. The high risk HPV group showed that the number of patients with AgNOR areas greater than 3.3 µm(2) and with expression of p16INK4a were statistically greater than the number of lower risk patients. None of the biopsies of LR-HPV carriers expressed p16 and AgNOR areas> 3.3 µm(2) simultaneously. Four LSILs and the NDE of this group expressed neither of the two markers. If the correlation between AgNOR areas and p16INK4a is good, we may be able to develop a low cost simple technology for studying patients infected with HR-HPV and diagnosed with LSIL of uncertain behavior.
Subject(s)
Antigens, Nuclear/metabolism , Carcinoma, Squamous Cell/pathology , Carcinoma, Squamous Cell/virology , Cyclin-Dependent Kinase Inhibitor p16/metabolism , Papillomaviridae/physiology , Uterine Cervical Dysplasia/pathology , Uterine Cervical Dysplasia/virology , Biomarkers, Tumor/metabolism , Female , Humans , Immunohistochemistry/economics , Papillomaviridae/isolation & purificationABSTRACT
Un objetivo de esta presentación es el de analizar las peculiaridades distintivas de la enzima lipasa proveniente de diferentes fuentes: gástrica (LG), intestinal (LI)hepática (LH), lipoproteica (LLP), pero, en especial, aquella de la pancreática (LP), sobre todo en lo relativo a sus interacciones neuro-hormonales.
Subject(s)
Humans , Gastrin-Secreting Cells , Estradiol , Laparotomy , Lipase , Micelles , Pancreas , Secretin , Somatostatin , TetragastrinABSTRACT
Un objetivo de esta presentación es el de analizar las peculiaridades distintivas de la enzima lipasa proveniente de diferentes fuentes: gástrica (LG), intestinal (LI)hepática (LH), lipoproteica (LLP), pero, en especial, aquella de la pancreática (LP), sobre todo en lo relativo a sus interacciones neuro-hormonales.(AU)
Subject(s)
Humans , Lipase/metabolism , Micelles , Laparotomy , Secretin/analysis , Gastrin-Secreting Cells/metabolism , Somatostatin/metabolism , Estradiol , Pancreas/pathology , TetragastrinABSTRACT
En este estudio se presenta el caso de un paciente pediátrico que luego de ser sometido a un transplante hepático con donante vivo relacionado, presentó un cuadro de hiperfosfatasemia alcalina sérica. Se pone énfasis en la importancia de la utilización de la técnica de isoelectroenfoque para determinar el origen tisular del hallazgo mencionado (AU)
Subject(s)
Humans , Female , Infant , Liver Transplantation/adverse effects , Immunosuppressive Agents/adverse effects , Alkaline Phosphatase/blood , Isoenzymes/blood , Graft Rejection/diagnosis , Bile Canaliculi/drug effects , Bile Canaliculi/enzymology , Isoelectric Focusing/trends , Liver Function Tests/methodsABSTRACT
En este estudio se presenta el caso de un paciente pediátrico que luego de ser sometido a un transplante hepático con donante vivo relacionado, presentó un cuadro de hiperfosfatasemia alcalina sérica. Se pone énfasis en la importancia de la utilización de la técnica de isoelectroenfoque para determinar el origen tisular del hallazgo mencionado
Subject(s)
Humans , Female , Infant , Alkaline Phosphatase/blood , Immunosuppressive Agents/adverse effects , Liver Transplantation/adverse effects , Bile Canaliculi , Bile Canaliculi/enzymology , Graft Rejection/diagnosis , Isoelectric Focusing/trends , Isoenzymes/blood , Liver Function Tests/methodsABSTRACT
In previous in vivo studies we have reported that atrial natriuretic factor enhanced induced salivary secretion and increased isoproterenol-induced amylase release in the rat suggesting that, ANF effect could be mediated by phosphatidylinositol hydrolysis. In the present work, the effect of ANF on rat parotid tissue incubated in vitro was investigated with the aim to assess whether the phosphoinositol pathway was involved in ANF intracellular signaling in the parotid gland. Results showed that ANF induced a dose dependent increase in amylase fractional release, which was lower than that evoked by any concentration of isoproterenol. Furthermore 100 nM ANF enhanced isoproterenol-evoked amylase release. The effect of ANF was not affected in the presence of propranolol suggesting the noninvolvement of the beta adrenergic receptor, which is the main stimulus for the output of the enzyme in the parotid gland. However, ANF increased phosphatidylinositol hydrolysis, which implies an increase in intracellular calcium, which is necessary for the achievement of maximal response in amylase release. This effect was abolished in the presence of neomycin supporting ANF direct stimulation of phospholipase C. These results suggest the involvement of the C type natriuretic peptide receptor coupled to phospholipase C in ANF evoked amylase release and ANF enhancement of the isoproterenol-induced output of the enzyme.
