The influence of exotic and native plants on illnesses with physical and spiritual causes in the semiarid region of Piauí, Northeast of Brazil.

BACKGROUND: Local medical systems (LMS) include native and exotic plants used for the treatment of diseases of physical and spiritual nature. The incorporation of exotic plants into these systems has been the subject of many studies. In this context, an analysis was conducted on the influence of the origin of plants on diseases of physical and spiritual nature in order to evaluate the therapeutic versatility of native and exotic species in these therapeutic targets, to investigate whether exotic plants mainly fill gaps not met by native plants (diversification hypothesis), and identify which species are prioritized in the redundant targets in these two therapeutic groups in the rural community of Morrinhos, Monsenhor Hipólito, Piauí. METHODS: Data collection took place in 2 stages. First, free lists and semi-structured interviews with local residents (n = 134) were conducted to survey plants used for therapeutic purposes and the associated illnesses. Then, another phase of interviews was carried out to evaluate the prioritization between native and exotic plants in redundant therapeutic targets. To test the diversification hypothesis (DH) in each group of illnesses, data were analyzed using generalized linear models (Poisson and Binomial GLMs); versatility was measured by the number of therapeutic indications and compared between resources using the Mann-Whitney test, and prioritization in each group was verified by comparing the proportions of native and exotic plants with the χ2 test. RESULTS: One hundred and thirty-two species of plants were surveyed, being 71 exotic and 61 native, with indications for physical and spiritual illnesses. The results revealed that the diversification hypothesis did not explain the inclusion of exotic plants in the local medical system to treat physical or spiritual illnesses and that the therapeutic versatility of exotic and native resources in the two groups was also similar (p > 0.05). However, exotic plants were prioritized in illnesses with physical causes and native plants in illnesses with spiritual causes. CONCLUSIONS: The local medical system presents similar and distinct patterns in the therapeutic targets, depending on the perspective evaluated. Therefore, it is necessary to investigate the patterns of use of medicinal plants in different sociocultural contexts in order to broaden the debate about the role of plant origin in the selection of treatments for illnesses with different causes.

Does prestige bias influence the recall and transmission of COVID-19-related information? Protocol registration for an experimental study conducted online.

In epidemic and pandemic contexts, such as that of COVID-19, epidemiological changes are continuous, and many people do not have access to accurate, up-to-date information. In this context, social learning can be an advantageous survival strategy. We investigate whether people remember and communicate information attributed to someone prestigious more often than that attributed to family members, politicians, and people with experience in public health. The experimental phase will include a recall stage and an information transmission stage, which will be based on a fictitious text containing an opinion about a drug treatment for COVID-19. There will be four versions of the text, and each participant will be assigned one of these versions for the investigation. The participants will be instructed to read the fictional story and then complete a distraction exercise. Subsequently, a recall test will be performed, where they will be asked to recount the story as accurately as possible. The second stage of the experiment is aimed at testing the transmission of information where we will conduct a linear chain transmission experiment, where eight chains of four participants will be used for each story. They will be asked to write down their recollection of the material. This text will undergo spelling error correction and then be sent to the next participant in the chain through the platform. At the end of the experiment, there will be a self-reporting questionnaire for the participants; this allows for triangulation of the data.

Synthesis and characterization of meloxicam eutectics with mandelic acid and saccharin for enhanced solubility.

Meloxicam (MLX) is a non-steroidal anti-inflammatory which is practically insoluble in water, requiring high concentrations to reach therapeutic levels and causing frequently gastrointestinal effects. In this way, the aim of this study was to synthesize two eutectic mixtures of MLX with mandelic acid (MND) and saccharin (SAC) by liquid-assisted grinding resulting in a multicomponent material with enhanced solubility. Mixtures were studied in different stoichiometric and eutectic point was found for each eutectic by Binary phase diagram and Tamman's triangle, with 0.33 molar fraction of MLX for SAC and MND. Eutectics were characterized by thermoanalytical techniques (TG-DSC, EGA, DSC, and DSC microscopy), infrared spectroscopy, and X-ray powder diffraction. Thermal behavior was studied and videos of the materials being heated were available. A polymorphic transition was discovered and studied for MLX-MND eutectic. Each new system was evaluated by solubility, dissolution, and hygroscopicity tests. Eutectics showed an increase in solubility of 1.7× (MLX-MND1), 3.1× (MLX-MND2), and 1.3× (MLX-SAC) with slower dissolution profile when compared with MLX. All new solid forms showed high hygroscopicity at 98% relative humidity with 27.9 and 58.9% increase in mass at day four for MLX-SAC and MLX-MND, deliquescence occurs at day 6. The experiments and analysis in this study help to understand the behavior of eutectics and evaluate them as an approach to modify properties in drugs.

Use of game fauna by Fulni-ô people in Northeastern Brazil: implications for conservation.

BACKGROUND: Due to the influence of several factors on the hunting of game meat, we investigated how the seasonality of the environment, the abundance, and the biomass of wild animals, as well as the proximity to these resources, can affect the hunting. METHODS: The research was developed with the Fulni-ô people in the municipality of Águas Belas, Agreste of Pernambuco, Northeast of Brazil. In order to do this, we applied snowball sampling to select the participants. Data from potentially useful game species were obtained from lists and semi-structured interviews to register their particular kind of uses, capture periods (daytime, night, or both), preferences, and perceived abundance. The hunters who allowed their game meat captured to be weighed and identified were followed for 1 year. RESULTS: Our records pointed to a vast repertoire of potentially hunting animals. However, we did not verify relationships between the abundance, seasonality, and biomass of the animals that were hunted by the Fulni-ô. We observed a total of 209,866 (kg) of game meat hunted in the studied group, belonging to 23 species, distributed in three taxonomic groups, the birds being the most representative group with 59% of total reported. CONCLUSION: Such consumption by the group is well below in terms of biomass when compared to other ethnic or local groups in other regions of Brazil, or in Caatinga areas, characterizing an activity much more of cultural character than subsistence. Also, the use of game meat among the Fulni-ô seems to be actively directed to the preferred species, suggesting that in the case of an urbanized indigenous community, where other sources of income are available, the demand for game meat is lower when compared to other ethnic groups.

Gender and Its Role in the Resilience of Local Medical Systems of the Fulni-ô People in NE Brazil: Effects on Structure and Functionality.

Ethnobotanical studies focused on understanding how local medical systems are functionally maintained suggest that utilitarian redundancy and knowledge transmission are factors that influence the resilience of the system. However, to date, there have not been any studies that analyze these factors in relation to the variables that influence the variation of knowledge. Given the above, this study aims to analyze the influence of gender in the resilience of the system, using utilitarian redundancy and knowledge transmission as factors. Information from 198 married couples (396 people) was collected from the indigenous community of Fulni-ô (NE Brazil). Knowledge between men and women was analyzed based on the total number of known plants, therapeutic targets, information units, utilitarian redundancy, models of transmission, and sharing for each gender. Fulni-ô men know a greater number of plants, therapeutic targets treated with plants, and information units than women. They also had greater utilitarian redundancy. However, regarding knowledge transmission, sharing among women was greater, transmission is related to gender, and there is no difference between the numbers of models of knowledge information. In the system of local medical knowledge, gender exerts an important role in the resilience of the system. This study shows that men have a greater contribution to the structure and function of the system; however, both genders contribute to the flow of information in the system, which makes both genders important in the feedback of information.

Religiosity/Spirituality Matters on Plant-Based Local Medical System.

Religiosity/spirituality can affect health and quality of life in myriad ways. Religion has been present since the first moments of our evolutionary history, whether it is understood as a byproduct or as an adaptation of our cognitive evolution. We investigated how religion influences medicinal plant-based local medical systems (LMSs) and focuses on how individual variation in the degree of religiosity/spirituality affects the structure of LMSs. The knowledge of people about their medical systems was obtained through the free-listing technique, and level of religiosity/spirituality was calculated using the Brazilian version of the Brief Multidimensional Measure of Religiousness/Spirituality. We employed a Generalized Linear Model to obtain the best model. Religiosity/spirituality is predictive of structural and functional aspects of medicinal plant-based LMSs. Our model encourages a discussion of the role of religion in the health of an individual as well as in the structure of an individual's support system. Religiosity/spirituality (and the dimensions of Commitment and Religious and Spiritual History, in particular) act to protect structural and functional elements of LMSs. By providing protection, the LMS benefits from greater resilience, at both the individual and population levels. We suggest that the socialization process resulting from the religious phenomenon has contributed to the complexity and maintenance of LMSs by means of the interaction of individuals as they engage in their religious observances, thus facilitating cultural transmission.

An ethnopharmacological assessment of the use of plants against parasitic diseases in humans and animals.

ETHNOPHARMACOLOGICAL RELEVANCE: Ethnobotanical surveys are detecting an increasing frequency of exotic plant species in pharmacopeias, which has led researchers to investigate the role of such species in traditional medical systems. According to the diversification hypothesis, exotic species are included to complete pharmacopeias, i.e., to treat diseases for which no native species are known, thus broadening the scope of the plant repertoire. MATERIALS AND METHODS: The present study was conducted at two rural communities in northeastern Brazil aiming at a survey of the plants known or used by the population to treat endo- and ectoparasitic diseases in humans and animals. In addition, plant extracts exhibiting acaricide activity were assessed using the engorged female immersion and larval packet tests (LPT). RESULTS: The results of the present study showed a tendency for native species to be used against ectoparasites and exhibit a broader scope of use compared to exotic species. In turn, exotic species were predominantly indicated to treat diseases caused by endoparasites, although there was an overlap of native and exotic species relative to some therapeutic purpose, e.g., ticks. Only two of the plant species tested exhibited acaricide activity (Nicotiana glauca Graham and Croton blanchetianus Baill.), and in both cases, the activity was weak. CONCLUSION: The ethnobotanical data do not fully support the suggested hypothesis. Overall, the wide versatility of exotic species was not exclusively used to treat parasitic diseases in humans and animals. In addition, the selection of acaricide plants based on the ethnopharmacological study generated uninteresting results.

Neuronal degeneration and gliosis time-course in the mouse hippocampal formation after pilocarpine-induced status epilepticus.

Temporal lobe epilepsy (TLE) is the most common type of human epilepsy and has been related with extensive loss of hippocampal pyramidal and dentate hilar neurons and gliosis. Many characteristics of TLE are reproduced in the pilocarpine model of epilepsy in mice. This study analyzed the neuronal damage, assessed with Fluoro-Jade (FJB) and cresyl violet, and gliosis, investigated with glial fibrilary acidic protein (GFAP) immunohistochemistry, occurring in the hippocampal formation of mice at 3, 6, 12 and 24h, 1 and 3 weeks after the pilocarpine-induced status-epilepticus (SE) onset. The maximum neuronal damage score and the FJB-positive neurons peak were found in the hilus of dentate gyrus 3 and 12 h after SE onset (P<0.05), respectively. At 1 week after SE onset, the greatest neuronal damage score was detected in the CA1 pyramidal cell layer and the greatest numbers of FJB-positive neurons were found both in the CA1 and CA3 pyramidal cell layers (P<0.05). The molecular, CA3 and CA1 pyramidal cell layers expressed highest presence of GFAP immunoreaction at 1 and 3 weeks after SE onset (P<0.05). Our findings show that, depending on the affected area, neuronal death and gliosis can occur within few hours or weeks after SE onset. Our results corroborate previous studies and characterize short time points of temporal evolution of neuropathological changes after the onset of pilocarpine-induced SE in mice and evidences that additional studies of this temporal evolution may be useful to the comprehension of the cellular mechanisms underlying epileptogenesis.