ABSTRACT
Dengue virus type 3 genotype I was detected in Brazil during epidemics in 2002-2004. To confirm this finding, we identified this virus genotype in naturally infected field-caught Aedes aegypti mosquitoes and eggs. Results showed usefulness of virus investigations in vectors as a component of active epidemiologic surveillance.
Subject(s)
Aedes/virology , Dengue Virus/genetics , Dengue/epidemiology , Ovum/virology , Animals , Brazil/epidemiology , Dengue/transmission , Dengue/virology , Dengue Virus/isolation & purification , Female , Genetic Variation , Genome, Viral , Humans , Infectious Disease Transmission, Vertical , Male , Phylogeny , RNA, Viral/analysis , RNA, Viral/genetics , Sequence Alignment , Sequence Analysis, RNA , Species Specificity , Time FactorsABSTRACT
Outbreaks of bovine vaccinia disease caused by circulation of Vaccinia virus (VACV) strains have been a common occurrence in Brazil in the recent years, being an important emergent zoonosis. During a single outbreak that took place in 2001, two genetically different VACV strains were isolated and named Guarani P1 virus (GP1V) and Guarani P2 virus (GP2V). Molecular diagnosis was done through restriction fragment length polymorphism (RFLP) of ati gene (A26L) and by sequence analysis of a group of five VACV genes including the C11R, J2R, A56R, B18R, and E3L genes. These findings confirmed the co-circulation of two different Vaccinia virus strains during the same outbreak, raising important questions about the origin, emergence, and circulation of VACV strains in Brazil.
Subject(s)
Cattle Diseases/virology , Disease Outbreaks , Vaccinia virus/isolation & purification , Vaccinia/veterinary , Animals , Brazil/epidemiology , Cattle , Cattle Diseases/epidemiology , Phylogeny , Species Specificity , Vaccinia/epidemiology , Vaccinia/virology , Vaccinia virus/classificationABSTRACT
Passatempo virus was isolated during a zoonotic outbreak. Biologic features and molecular characterization of hemagglutinin, thymidine kinase, and vaccinia growth factor genes suggested a vaccinia virus infection, which strengthens the idea of the reemergence and circulation of vaccinia virus in Brazil. Molecular polymorphisms indicated that Passatempo virus is a different isolate.
