ABSTRACT
It is well established that helper T cell responses influence resistance or susceptibility to Mycobacterium leprae infection, but the role of more recently described helper T cell subsets in determining severity is less clear. To investigate the involvement of Th17 cells in the pathogenesis of leprosy, we determined the immune profile with variant presentations of leprosy. Firstly, IL-17A, IFN-γ and IL-10 were evaluated in conjunction with CD4+ T cell staining by confocal microscopy of lesion biopsies from tuberculoid (TT) and lepromatous leprosy (LL) patients. Secondly, inflammatory cytokines were measured by multiplex assay of serum samples from Multibacillary (MB, n = 28) and Paucibacillary (PB, n = 23) patients and household contacts (HHC, n = 23). Patients with leprosy were also evaluated for leprosy reaction occurrence: LR+ (n = 8) and LR- (n = 20). Finally, peripheral blood mononuclear cells were analysed by flow cytometry used to determine the phenotype of cytokine-producing cells. Lesions from TT patients were found to have more CD4+ IL-17A+ cells than those from LL patients. Higher concentrations of IL-17A and IL-1ß were observed in serum from PB than MB patients. The highest serum IFN-γ concentrations were, however, detected in sera from MB patients that developed leprosy reactions (MB LR+ ). Together, these results indicate that Th1 cells were associated with both the PB presentation and also with leprosy reactions. In contrast, Th17 cells were associated with an effective inflammatory response that is present in the PB forms but were not predictive of leprosy reactions in MB patients.
Subject(s)
Inflammation Mediators/immunology , Leprosy, Paucibacillary/immunology , Leprosy/immunology , Mycobacterium leprae/immunology , Th1 Cells/immunology , Th17 Cells/immunology , Adolescent , Adult , Aged , Aged, 80 and over , CD4-Positive T-Lymphocytes/immunology , CD4-Positive T-Lymphocytes/metabolism , Child , Contact Tracing , Female , Flow Cytometry , Humans , Inflammation Mediators/blood , Inflammation Mediators/metabolism , Interferon-gamma/blood , Interferon-gamma/immunology , Interferon-gamma/metabolism , Interleukin-10/blood , Interleukin-10/immunology , Interleukin-10/metabolism , Interleukin-17/blood , Interleukin-17/immunology , Interleukin-17/metabolism , Interleukin-1beta/blood , Interleukin-1beta/immunology , Interleukin-1beta/metabolism , Leprosy/blood , Leprosy/microbiology , Leprosy, Multibacillary/blood , Leprosy, Multibacillary/immunology , Leprosy, Multibacillary/microbiology , Leprosy, Paucibacillary/blood , Leprosy, Paucibacillary/microbiology , Male , Microscopy, Confocal , Middle Aged , Mycobacterium leprae/physiology , Th1 Cells/metabolism , Th17 Cells/metabolism , Young AdultABSTRACT
Central nervous system involvement by tuberculosis presents two main types: tuberculosis meningitis or tuberculoma. This second condition has nowadays ideal conditions for development. We report three patients who developed paradoxical brain tuberculosis expansive lesion during the use of tuberculostatic drugs. The cases point out the importance of tomography follow up of patients who present neurological signs in the course of treatment for tuberculosis. The first two patients presented onset of the a neurological symptoms, associated with lung tuberculosis. The third patient previously had tuberculous meningitis. Computerized tomography was used to follow up these patients. Tuberculostatic and corticoid drugs were used for treatment.
