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Expression of 5-lipoxygenase in lymphoblastoid B and T cells.
el Makhour-Hojeij, Y; Baclet, M C; Chable-Rabinovitch, H; Beneytout, J L; Cook, J.
Prostaglandins ; 48(1): 21-9, 1994 Jul.
Article in English | MEDLINE | ID: mdl-7972877

ABSTRACT

The expression of arachidonate 5-lipoxygenase (5-LOX, arachidonate: oxygen 5-oxidoreductase; EC 1.13.11.34) and the 5-lipoxygenase activating protein (FLAP) genes in lymphoblastoid B and T cell lines was studied at the transcriptional level by reverse transcription-PCR analysis. Two B cell lines, CESS and SKW 6.4, were found to express both 5-LOX and FLAP mRNA. One T cell line, MOLT 4, expressed only FLAP mRNA. Upon stimulation of intact cells by calcium ionophore, B lymphoblastoid cells produced 5, 12 and 15 hydroxyeicosatetraenoic acids as well as leukotriene B4.


Subject(s)
Arachidonate 5-Lipoxygenase/biosynthesis , B-Lymphocytes/metabolism , Carrier Proteins/biosynthesis , Membrane Proteins/biosynthesis , T-Lymphocytes/metabolism , 5-Lipoxygenase-Activating Proteins , Arachidonate 5-Lipoxygenase/genetics , Arachidonic Acid/metabolism , B-Lymphocytes/enzymology , Base Sequence , Carrier Proteins/genetics , Cell Line , Gene Expression , Humans , Membrane Proteins/genetics , Molecular Sequence Data , Polymerase Chain Reaction , RNA, Messenger/analysis , T-Lymphocytes/enzymology , Transcription, Genetic
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